Surface Modifications to Promote the Osteoconductivity of Ultra-high-molecular-weight-polyethylene Fabrics for a Novel Biomimetic Artificial Disc Prosthesis: An in Vitro Study

Overview

Journal J Biomed Mater Res B Appl Biomater

Specialty Biomedical Engineering

Date 2022 Sep 16

PMID 36111647

Authors

Celien A M Jacobs

Esther E A Cramer

Aylvin A Dias

Harold Smelt

Sandra Hofmann

Keita Ito

Affiliations

Soon will be listed here.

Abstract

A novel biomimetic artificial intervertebral disc (bioAID) for the cervical spine was developed, containing a hydrogel core representing the nucleus pulposus, an UHMWPE fiber jacket as annulus fibrosis, and titanium endplates with pins for mechanical fixation. Osseointegration of the UHMWPE fibers to adjacent bone structures is required to achieve proper biomimetic behavior and to provide long-term stability. Therefore, the aim of this study was to assess the osteoconductivity of several surface modifications of UHMWPE fabrics, 2D weft-knitted, using non-treated UHMWPE fibers (N), plasma treated UHMWPE fibers (PT), 10% hydroxy apatite (HA) loaded UHMWPE fibers (10%HA), plasma treated 10%HA UHMWPE fibers (PT-10%HA), 15%HA loaded UHMWPE fibers (15%HA) and plasma treated 15%HA UHMWPE fibers (PT-15%HA). Scanning electron microscopy (SEM) was used for surface characterization. Biological effects were assessed by evaluating initial cell attachment (SEM, DNA content), metabolic activity (PrestoBlue assay), proliferation, differentiation (alkaline phosphatase activity) and mineralization (energy dispersive x-ray, EDX analysis) using human bone marrow stromal cells. Plasma treated samples showed increased initial cell attachment, indicating the importance of hydrophilicity for cell attachment. However, incorporation only of HA or plasma treatment alone was not sufficient to result in upregulated alkaline phosphatase activity (ALP) activity. Combining HA loaded fibers with plasma treatment showed a combined effect, leading to increased cell attachment and upregulated ALP activity. Based on these results, combination of HA loaded UHMWPE fibers and plasma treatment provided the most promising fabric surface for facilitating bone ingrowth.

Citing Articles

Surface modifications to promote the osteoconductivity of ultra-high-molecular-weight-polyethylene fabrics for a novel biomimetic artificial disc prosthesis: An in vitro study.

Jacobs C, Cramer E, Dias A, Smelt H, Hofmann S, Ito K J Biomed Mater Res B Appl Biomater. 2022; 111(2):442-452.

PMID: 36111647 PMC: 10087191. DOI: 10.1002/jbm.b.35163.

References

Surmenev R, Surmeneva M, Ivanova A . Significance of calcium phosphate coatings for the enhancement of new bone osteogenesis--a review. Acta Biomater. 2013; 10(2):557-79. DOI: 10.1016/j.actbio.2013.10.036. View

Senatov F, Amanbek G, Orlova P, Bartov M, Grunina T, Kolesnikov E . Biomimetic UHMWPE/HA scaffolds with rhBMP-2 and erythropoietin for reconstructive surgery. Mater Sci Eng C Mater Biol Appl. 2020; 111:110750. DOI: 10.1016/j.msec.2020.110750. View

Gloria A, Causa F, De Santis R, Netti P, Ambrosio L . Dynamic-mechanical properties of a novel composite intervertebral disc prosthesis. J Mater Sci Mater Med. 2007; 18(11):2159-65. DOI: 10.1007/s10856-007-3003-z. View

Cai Y, Wang X, Poh C, Tan H, Soe M, Zhang S . Accelerated bone growth in vitro by the conjugation of BMP2 peptide with hydroxyapatite on titanium alloy. Colloids Surf B Biointerfaces. 2013; 116:681-6. DOI: 10.1016/j.colsurfb.2013.11.004. View

Macuvele D, Nones J, Matsinhe J, Lima M, Soares C, Fiori M . Advances in ultra high molecular weight polyethylene/hydroxyapatite composites for biomedical applications: A brief review. Mater Sci Eng C Mater Biol Appl. 2017; 76:1248-1262. DOI: 10.1016/j.msec.2017.02.070. View

Gittens R, McLachlan T, Olivares-Navarrete R, Cai Y, Berner S, Tannenbaum R . The effects of combined micron-/submicron-scale surface roughness and nanoscale features on cell proliferation and differentiation. Biomaterials. 2011; 32(13):3395-403. PMC: 3350795. DOI: 10.1016/j.biomaterials.2011.01.029. View

Yassin M, Leknes K, Pedersen T, Xing Z, Sun Y, Lie S . Cell seeding density is a critical determinant for copolymer scaffolds-induced bone regeneration. J Biomed Mater Res A. 2015; 103(11):3649-58. PMC: 4744655. DOI: 10.1002/jbm.a.35505. View

Nobles K, Janorkar A, Williamson R . Surface modifications to enhance osseointegration-Resulting material properties and biological responses. J Biomed Mater Res B Appl Biomater. 2021; 109(11):1909-1923. DOI: 10.1002/jbm.b.34835. View

Bitar M, Brown R, Salih V, Kidane A, Knowles J, Nazhat S . Effect of cell density on osteoblastic differentiation and matrix degradation of biomimetic dense collagen scaffolds. Biomacromolecules. 2007; 9(1):129-35. DOI: 10.1021/bm701112w. View

10.

Kim K, Dean D, Mikos A, Fisher J . Effect of initial cell seeding density on early osteogenic signal expression of rat bone marrow stromal cells cultured on cross-linked poly(propylene fumarate) disks. Biomacromolecules. 2009; 10(7):1810-7. PMC: 3655530. DOI: 10.1021/bm900240k. View

11.

Roy M, Bandyopadhyay A, Bose S . Induction Plasma Sprayed Nano Hydroxyapatite Coatings on Titanium for Orthopaedic and Dental Implants. Surf Coat Technol. 2011; 205(8-9):2785-2792. PMC: 3086534. DOI: 10.1016/j.surfcoat.2010.10.042. View

12.

Habibovic P, Yuan H, van der Valk C, Meijer G, van Blitterswijk C, de Groot K . 3D microenvironment as essential element for osteoinduction by biomaterials. Biomaterials. 2004; 26(17):3565-75. DOI: 10.1016/j.biomaterials.2004.09.056. View

13.

van den Broek P, Huyghe J, Ito K . Biomechanical behavior of a biomimetic artificial intervertebral disc. Spine (Phila Pa 1976). 2011; 37(6):E367-73. DOI: 10.1097/BRS.0b013e3182326305. View

14.

Wennerberg A, Jimbo R, Stubinger S, Obrecht M, Dard M, Berner S . Nanostructures and hydrophilicity influence osseointegration: a biomechanical study in the rabbit tibia. Clin Oral Implants Res. 2013; 25(9):1041-50. DOI: 10.1111/clr.12213. View

15.

van den Broek P, Huyghe J, Wilson W, Ito K . Design of next generation total disk replacements. J Biomech. 2011; 45(1):134-40. DOI: 10.1016/j.jbiomech.2011.09.017. View

16.

Senatov F, Maksimkin A, Chubrik A, Kolesnikov E, Orlova P, Krivozubov M . Osseointegration evaluation of UHMWPE and PEEK-based scaffolds with BMP-2 using model of critical-size cranial defect in mice and push-out test. J Mech Behav Biomed Mater. 2021; 119:104477. DOI: 10.1016/j.jmbbm.2021.104477. View

17.

Bigi A, Nicoli-Aldini N, Bracci B, Zavan B, Boanini E, Sbaiz F . In vitro culture of mesenchymal cells onto nanocrystalline hydroxyapatite-coated Ti13Nb13Zr alloy. J Biomed Mater Res A. 2007; 82(1):213-21. DOI: 10.1002/jbm.a.31132. View

18.

Junker R, Dimakis A, Thoneick M, Jansen J . Effects of implant surface coatings and composition on bone integration: a systematic review. Clin Oral Implants Res. 2009; 20 Suppl 4:185-206. DOI: 10.1111/j.1600-0501.2009.01777.x. View

19.

Kim J, Lin C, Stavre Z, Greenblatt M, Shim J . Osteoblast-Osteoclast Communication and Bone Homeostasis. Cells. 2020; 9(9). PMC: 7564526. DOI: 10.3390/cells9092073. View

20.

Vohra S, Hennessy K, Sawyer A, Zhuo Y, Bellis S . Comparison of mesenchymal stem cell and osteosarcoma cell adhesion to hydroxyapatite. J Mater Sci Mater Med. 2008; 19(12):3567-74. PMC: 3677517. DOI: 10.1007/s10856-008-3525-z. View