MiR-92a-3p Promotes Breast Cancer Proliferation by Regulating the KLF2/BIRC5 Axis

Overview

Journal Thorac Cancer

Specialties Oncology
Pulmonary Medicine

Date 2022 Sep 13

PMID 36100919

Authors

Zhi-Hao Yu

Zhao-Hui Chen

Guang-Lei Zhou

Xue-Jie Zhou

Hai-Yan Ma

Yue Yu

Xin Wang

Xu-Chen Cao

Affiliations

Soon will be listed here.

Abstract

Background: Breast cancer remains the most common malignancy in females around the world. Recently, a growing number of studies have focused on gene dysregulation. In our previous study, Krüppel-like factors (KLFs) were found to play essential roles in breast cancer development, among which KLF2 could function as a tumor suppressor. Nevertheless, the underlying molecular mechanism remains unclear.

Methods: miR-92a-3p was identified as the upstream regulator of KLF2 by starBase v.3.0. The regulation of KLF2 by miR-92a-3p was verified by a series of in vitro and in vivo assays. Further exploration revealed that Baculoviral IAP Repeat Containing 5 (BIRC5) was the target of KLF2. ChIP assay, dual-luciferase reporter analysis, quantitative real-time PCR, and western blot were performed for verification.

Results: miR-92a-3p functioned as a tumor promoter by inhibiting KLF2 by binding to its 3'-untranslated region (3'-UTR). In addition, KLF2 could transcriptionally suppress the expression of BIRC5.

Conclusion: Collectively, our results uncovered the miR-92a-3p/KLF2/BIRC5 axis in breast cancer and provided a potential mechanism for breast cancer development, which may serve as promising strategies for breast cancer therapy.

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