» Articles » PMID: 36098743

Zbtb34 Promotes Embryonic Stem Cell Proliferation by Elongating Telomere Length

Overview
Specialty Geriatrics
Date 2022 Sep 13
PMID 36098743
Authors
Affiliations
Soon will be listed here.
Abstract

Zbtb34 is a novel zinc finger protein, which is revealed by biological software analysis to have 3 zinc fingers, but its functions remain unknown. In this study, mouse cDNA was amplified by PCR and inserted into the plasmid pEGFP-N1 to generate Zbtb34-EGFP fusion protein. The upregulation of Zbtb34 in mouse embryonic stem cells promoted telomere elongation and increased cell proliferation. In order to understand the above phenomena, the telomere co-immunoprecipitation technique was employed to investigate the relationship between Zbtb34 and telomeres. The results indicated that Zbtb34 could bind to the DNA sequences of the telomeres. Alanine substitution of the third zinc finger abolished such binding. Since Pot1 is the only protein binding to the single-stranded DNA at the end of the telomeres, we further investigated the relationship between Zbtb34 and Pot1. The results revealed that the upregulation of Zbtb34 decreased the binding of Pot1b to the telomeres. Through the upregulation of Pot1b, the binding of Zbtb34 to the telomeres was also reduced. In conclusion, we showed that the main biological function of Zbtb34 was to bind telomere DNA via its third ZnF, competing with Pot1b for the binding sites, resulting in telomere elongation and cell proliferation.

Citing Articles

The nutritional roles of zinc for immune system and COVID-19 patients.

Jin D, Wei X, He Y, Zhong L, Lu H, Lan J Front Nutr. 2024; 11:1385591.

PMID: 38706559 PMC: 11066294. DOI: 10.3389/fnut.2024.1385591.


Expression profiles of oviductal mRNAs and lncRNAs in the follicular phase and luteal phase of sheep (Ovis aries) with 2 fecundity gene (FecB) genotypes.

Chen W, Li Z, Zhong R, Sun W, Chu M G3 (Bethesda). 2023; 14(1).

PMID: 38051961 PMC: 10755197. DOI: 10.1093/g3journal/jkad270.


ZBTB34 is a hepatocellular carcinoma-associated protein with a monopartite nuclear localization signal.

Liu Z, Jin D, Wei X, Gao Y, Gao X, Li X Aging (Albany NY). 2023; 15(16):8487-8500.

PMID: 37650557 PMC: 10496988. DOI: 10.18632/aging.204987.

References
1.
Smith E, Pendlebury D, Nandakumar J . Structural biology of telomeres and telomerase. Cell Mol Life Sci. 2019; 77(1):61-79. PMC: 6986361. DOI: 10.1007/s00018-019-03369-x. View

2.
Lazzerini-Denchi E, Sfeir A . Stop pulling my strings - what telomeres taught us about the DNA damage response. Nat Rev Mol Cell Biol. 2016; 17(6):364-78. PMC: 5385261. DOI: 10.1038/nrm.2016.43. View

3.
Guerrero E, Vega S, Fu C, De Leon R, Beltran D, Solis M . Increased proliferation and differentiation capacity of placenta-derived mesenchymal stem cells from women of median maternal age correlates with telomere shortening. Aging (Albany NY). 2021; 13(22):24542-24559. PMC: 8660609. DOI: 10.18632/aging.203724. View

4.
Kasiappan R, Jutooru I, Mohankumar K, Karki K, Lacey A, Safe S . Reactive Oxygen Species (ROS)-Inducing Triterpenoid Inhibits Rhabdomyosarcoma Cell and Tumor Growth through Targeting Sp Transcription Factors. Mol Cancer Res. 2019; 17(3):794-805. PMC: 6397684. DOI: 10.1158/1541-7786.MCR-18-1071. View

5.
Hedrick E, Crose L, Linardic C, Safe S . Histone Deacetylase Inhibitors Inhibit Rhabdomyosarcoma by Reactive Oxygen Species-Dependent Targeting of Specificity Protein Transcription Factors. Mol Cancer Ther. 2015; 14(9):2143-53. PMC: 4618474. DOI: 10.1158/1535-7163.MCT-15-0148. View