A Simple Electrochemical Aptasensor for Saxitoxin Detection

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 Sep 12

PMID 36093254

Authors

Weixian Zheng

Xinyu Liu

Qianwen Li

Zuju Shu

Zhongbo Li

Lijun Zhang

Affiliations

Soon will be listed here.

Abstract

In this study, a simple electrochemical aptasensor for saxitoxin (STX) detection was developed based on a short anti-STX aptamer with a methylene blue (MB) incorporated at the 3'-end. The aptamer was immobilized on the surface of gold electrode and served as the recognition element. The aptasensor was successfully used as a highly selective and sensitive biosensor for STX detection because of the selection of aptamer along with the excellent electrocatalytical properties of MB. With the increase of the dynamic concentration of STX from 1 nM to 1 μM, the aptasensor exhibited a sensitivity with a peak current change of 2.92 μA per decade and a low detection limit of 1 nM. In addition, the aptasensor demonstrated excellent selectivity to STX, which prevented the interferences from neo-STX, okadaic acid and common metal ions. Using the proposed principle, several other aptasensors with improved performance can be constructed the modification of the electrochemical sensor with appropriate aptamers.

Citing Articles

Advances in Biosensors for the Rapid Detection of Marine Biotoxins: Current Status and Future Perspectives.

Zhu X, Zhao Y, Wu L, Gao X, Huang H, Han Y Biosensors (Basel). 2024; 14(4).

PMID: 38667196 PMC: 11048312. DOI: 10.3390/bios14040203.

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