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Nuclease-assisted Target Recycling Signal Amplification Strategy for Graphene Quantum Dot-based Fluorescent Detection of Marine Biotoxins

Overview
Journal Mikrochim Acta
Specialties Biotechnology
Chemistry
Date 2021 Mar 9
PMID 33687572
Citations 5
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Abstract

Saxitoxin (STX) is a major marine toxin from shellfish, and it is responsible for paralytic shellfish poisoning (PSP). In this study, a highly sensitive and rapid aptamer assay was developed for STX detection by combining fluorescence resonance energy transfer (FRET) and nuclease-assisted target recycling signal amplification. The aptamer STX-41 conjugated with graphene quantum dots (GQDs) was adsorbed on magnetic reduced graphene oxide (MRGO) to establish a fluorescence quenching system. Then, the binding between STX and aptamer induced the desorption of GQD-aptamer from MRGO and the restoring of fluorescence for the fluorescent determination of STX. The digestion of the target bound aptamer by DNase I could release the target for recycling thus achieving signal amplification. Under the optimized conditions, the aptamer assay showed a wide detection range (0.1-100 ng·mL), low detection limit (LOD of 0.035 ng·mL), high specificity, good recovery (86.75-94.08% in STX-spiked clam samples) and repeatability (RSD of 4.27-7.34%). Combined with fluorescent detection technology, signal amplification technology, and magnetic separation technology, the proposed method can be used to detect STX in seafood products successfully.

Citing Articles

Development of a Quick and Highly Sensitive Amplified Luminescent Proximity Homogeneous Assay for Detection of Saxitoxin in Shellfish.

Zhao C, Zhang Z, Li J, Lu Y, Ma F, Wang Z Toxins (Basel). 2024; 16(8).

PMID: 39195751 PMC: 11360761. DOI: 10.3390/toxins16080341.


Screening biotoxin aptamer and their application of optical aptasensor in food stuff: a review.

Sun J, Zhang M, Gao Q, Shao B Front Chem. 2024; 12:1425774.

PMID: 39114265 PMC: 11303198. DOI: 10.3389/fchem.2024.1425774.


Advances in Biosensors for the Rapid Detection of Marine Biotoxins: Current Status and Future Perspectives.

Zhu X, Zhao Y, Wu L, Gao X, Huang H, Han Y Biosensors (Basel). 2024; 14(4).

PMID: 38667196 PMC: 11048312. DOI: 10.3390/bios14040203.


Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors.

Lee M, Shin S, Kim S, Park N Molecules. 2023; 28(21).

PMID: 37959747 PMC: 10647268. DOI: 10.3390/molecules28217327.


A simple electrochemical aptasensor for saxitoxin detection.

Zheng W, Liu X, Li Q, Shu Z, Li Z, Zhang L RSC Adv. 2022; 12(37):23801-23807.

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