A Heat Shock 70kDa Protein MaltHSP70-2 Contributes to Thermal Resistance in Monochamus Alternatus (Coleoptera: Cerambycidae): Quantification, Localization, and Functional Analysis

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2022 Sep 10

PMID 36088287

Authors

Hui Li

Shouyin Li

Jin Chen

Lulu Dai

Ruixu Chen

Jianren Ye

Dejun Hao

Affiliations

Soon will be listed here.

Abstract

Background: Heat Shock Proteins 70 (HSP70s) in insects act on a diverse range of substrates to assist with overcoming extreme high temperatures. MaltHSP70-2, a member of HSP70s, has been characterized to involve in the thermotolerance of Monochamus alternatus in vitro, while quantification and localization of MaltHSP70-2 in various tissues and its functional analysis in vivo remain unclear.

Results: In this study, temporal expression of MaltHSP70-2 indicated a long-last inductive effect on MaltHSP70-2 expression maintained 48 hours after heat shock. MaltHSP70-2 showed a global response to heat exposure which occurring in various tissues of both males and females. Particularly in the reproductive tissues, we further performed the quantification and localization of MaltHSP70-2 protein using Western Blot and Immunohistochemistry, suggesting that enriched MaltHSP70-2 in the testis (specifically in the primary spermatocyte) must be indispensable to protect the reproductive activities (e.g., spermatogenesis) against high temperatures. Furthermore, silencing MaltHSP70-2 markedly influenced the expression of other HSP genes and thermotolerance of adults in bioassays, which implied a possible interaction of MaltHSP70-2 with other HSP genes and its role in thermal resistance of M. alternatus adults.

Conclusions: These findings shed new insights into thermo-resistant mechanism of M. alternatus to cope with global warming from the perspective of HSP70s functions.

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References

Jin J, Zhao M, Wang Y, Zhou Z, Wan F, Guo J . Induced Thermotolerance and Expression of Three Key Genes (, , and ) and Their Roles in the High Temperature Tolerance of . Front Physiol. 2020; 10:1593. PMC: 6971057. DOI: 10.3389/fphys.2019.01593. View

Bahar M, Hegedus D, Soroka J, Coutu C, Bekkaoui D, Dosdall L . Survival and hsp70 gene expression in Plutella xylostella and its larval parasitoid Diadegma insulare varied between slowly ramping and abrupt extreme temperature regimes. PLoS One. 2013; 8(9):e73901. PMC: 3765401. DOI: 10.1371/journal.pone.0073901. View

Zhu K, Palli S . Mechanisms, Applications, and Challenges of Insect RNA Interference. Annu Rev Entomol. 2019; 65:293-311. PMC: 9939233. DOI: 10.1146/annurev-ento-011019-025224. View

Mahroof R, Zhu K, Neven L, Subramanyam B, Bai J . Expression patterns of three heat shock protein 70 genes among developmental stages of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae). Comp Biochem Physiol A Mol Integr Physiol. 2005; 141(2):247-56. DOI: 10.1016/j.cbpb.2005.05.044. View

Song Y, Fee L, Lee T, Wharton R . The molecular chaperone Hsp90 is required for mRNA localization in Drosophila melanogaster embryos. Genetics. 2007; 176(4):2213-22. PMC: 1950626. DOI: 10.1534/genetics.107.071472. View

Lezzi M, Meyer B, Mahr R . Heat shock phenomena in Chironomus tentans I. In vivo effects of heat, overheat, and quenching on salivary chromosome puffing. Chromosoma. 1981; 83(3):327-39. DOI: 10.1007/BF00327356. View

Rosenzweig R, Nillegoda N, Mayer M, Bukau B . The Hsp70 chaperone network. Nat Rev Mol Cell Biol. 2019; 20(11):665-680. DOI: 10.1038/s41580-019-0133-3. View

Lancaster L, Dudaniec R, Chauhan P, Wellenreuther M, Svensson E, Hansson B . Gene expression under thermal stress varies across a geographical range expansion front. Mol Ecol. 2016; 25(5):1141-56. DOI: 10.1111/mec.13548. View

Ma C, Ma G, Pincebourde S . Survive a Warming Climate: Insect Responses to Extreme High Temperatures. Annu Rev Entomol. 2020; 66:163-184. DOI: 10.1146/annurev-ento-041520-074454. View

10.

Elekonich M . Extreme thermotolerance and behavioral induction of 70-kDa heat shock proteins and their encoding genes in honey bees. Cell Stress Chaperones. 2008; 14(2):219-26. PMC: 2727992. DOI: 10.1007/s12192-008-0063-z. View

11.

Hu S, Ning T, Fu D, Haack R, Zhang Z, Chen D . Dispersal of the Japanese pine sawyer, Monochamus alternatus (Coleoptera: Cerambycidae), in mainland China as inferred from molecular data and associations to indices of human activity. PLoS One. 2013; 8(2):e57568. PMC: 3585188. DOI: 10.1371/journal.pone.0057568. View

12.

Mayer M, Gierasch L . Recent advances in the structural and mechanistic aspects of Hsp70 molecular chaperones. J Biol Chem. 2018; 294(6):2085-2097. PMC: 6369304. DOI: 10.1074/jbc.REV118.002810. View

13.

Ding D, Parkhurst S, Halsell S, Lipshitz H . Dynamic Hsp83 RNA localization during Drosophila oogenesis and embryogenesis. Mol Cell Biol. 1993; 13(6):3773-81. PMC: 359859. DOI: 10.1128/mcb.13.6.3773-3781.1993. View

14.

Li H, Zhao X, Qiao H, He X, Tan J, Hao D . Comparative Transcriptome Analysis of the Heat Stress Response in Hope (Coleoptera: Cerambycidae). Front Physiol. 2020; 10:1568. PMC: 6985590. DOI: 10.3389/fphys.2019.01568. View

15.

Li K, Gong Z . Feeling Hot and Cold: Thermal Sensation in Drosophila. Neurosci Bull. 2016; 33(3):317-322. PMC: 5567507. DOI: 10.1007/s12264-016-0087-9. View

16.

Deutsch C, Tewksbury J, Huey R, Sheldon K, Ghalambor C, Haak D . Impacts of climate warming on terrestrial ectotherms across latitude. Proc Natl Acad Sci U S A. 2008; 105(18):6668-72. PMC: 2373333. DOI: 10.1073/pnas.0709472105. View

17.

Li H, Qiao H, Liu Y, Li S, Tan J, Hao D . Characterization, expression profiling, and thermal tolerance analysis of heat shock protein 70 in pine sawyer beetle, hope (Coleoptera: Cerambycidae). Bull Entomol Res. 2020; 111(2):217-228. DOI: 10.1017/S0007485320000541. View

18.

Barbagallo B, Garrity P . Temperature sensation in Drosophila. Curr Opin Neurobiol. 2015; 34:8-13. PMC: 4508239. DOI: 10.1016/j.conb.2015.01.002. View

19.

Ju R, Luo Q, Gao L, Yang J, Li B . Identification of HSP70 gene in Corythucha ciliata and its expression profiles under laboratory and field thermal conditions. Cell Stress Chaperones. 2017; 23(2):195-201. PMC: 5823808. DOI: 10.1007/s12192-017-0840-7. View

20.

Zatsepina O, Nikitina E, Shilova V, Chuvakova L, Sorokina S, Vorontsova J . Hsp70 affects memory formation and behaviorally relevant gene expression in Drosophila melanogaster. Cell Stress Chaperones. 2021; 26(3):575-594. PMC: 8065088. DOI: 10.1007/s12192-021-01203-7. View