Nutritional Value of Eggplant Cultivars and Association with Sequence Variation in Genes Coding for Major Phenolics

Overview

Journal Plants (Basel)

Date 2022 Sep 9

PMID 36079649

Authors

Vasileia Chioti

Konstantina Zeliou

Aikaterini Bakogianni

Charikleia Papaioannou

Antonis Biskinis

Constantinos Petropoulos

Fotini N Lamari

Vasileios Papasotiropoulos

Affiliations

Soon will be listed here.

Abstract

Eggplant is a widely consumed vegetable, with significant nutritional value and high antioxidant content, mainly due to its phenolic constituents. Our goal was to determine the levels of carbohydrates, proteins, total phenolics, anthocyanins, flavonoids, chlorogenic acid, and the antioxidant capacity in thirteen eggplant cultivars cultivated in Greece and to identify sequence polymorphisms in key regulating genes of the phenylpropanoid pathway (, , , , , , ), which might relate to the phytochemical content of those cultivars. The carbohydrates' content differs among and within cultivars, while the rest of the phytochemicals differ only among cultivars. The cultivars 'EMI' and 'Lagkada' scored higher than the rest in phenolics, anthocyanins, ascorbic acid, caffeoylquinic acid, and antioxidant capacity. Moreover, significant correlations were observed between various ingredients and the antioxidant capacity (FRAP and DPPH). Sequence analysis revealed several SNPs in , , , , and among the cultivars studied. According to chi-square and logistic regression analyses, the missense mutation 4-108 correlates significantly with flavonoids, anthocyanins, and proteins; the synonymous mutation -105 correlates with anthocyanins and ascorbic acid; the missense mutation -438 correlates with flavonoids and chlorogenic acid, while the missense mutation 1-65 correlates with anthocyanins and sugars. These polymorphisms can be potentially utilized as molecular markers in eggplant breeding, while our data also contribute to the study of eggplant's secondary metabolism and antioxidant properties.

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References

Madeira F, Pearce M, Tivey A, Basutkar P, Lee J, Edbali O . Search and sequence analysis tools services from EMBL-EBI in 2022. Nucleic Acids Res. 2022; 50(W1):W276-W279. PMC: 9252731. DOI: 10.1093/nar/gkac240. View

Martinez-Ispizua E, Calatayud A, Marsal J, Mateos-Fernandez R, Diez M, Soler S . Phenotyping Local Eggplant Varieties: Commitment to Biodiversity and Nutritional Quality Preservation. Front Plant Sci. 2021; 12:696272. PMC: 8281111. DOI: 10.3389/fpls.2021.696272. View

Clifford M, Jaganath I, Ludwig I, Crozier A . Chlorogenic acids and the acyl-quinic acids: discovery, biosynthesis, bioavailability and bioactivity. Nat Prod Rep. 2017; 34(12):1391-1421. DOI: 10.1039/c7np00030h. View

Zhang Y, Hu Z, Chu G, Huang C, Tian S, Zhao Z . Anthocyanin accumulation and molecular analysis of anthocyanin biosynthesis-associated genes in eggplant (Solanum melongena L.). J Agric Food Chem. 2014; 62(13):2906-12. DOI: 10.1021/jf404574c. View

Butelli E, Titta L, Giorgio M, Mock H, Matros A, Peterek S . Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nat Biotechnol. 2008; 26(11):1301-8. DOI: 10.1038/nbt.1506. View

Babicki S, Arndt D, Marcu A, Liang Y, Grant J, Maciejewski A . Heatmapper: web-enabled heat mapping for all. Nucleic Acids Res. 2016; 44(W1):W147-53. PMC: 4987948. DOI: 10.1093/nar/gkw419. View

Kaushik P, Gramazio P, Vilanova S, Raigon M, Prohens J, Plazas M . Phenolics content, fruit flesh colour and browning in cultivated eggplant, wild relatives and interspecific hybrids and implications for fruit quality breeding. Food Res Int. 2017; 102:392-401. DOI: 10.1016/j.foodres.2017.09.028. View

Chen M, Xu M, Xiao Y, Cui D, Qin Y, Wu J . Fine Mapping Identifies SmFAS Encoding an Anthocyanidin Synthase as a Putative Candidate Gene for Flower Purple Color in Solanum melongena L. Int J Mol Sci. 2018; 19(3). PMC: 5877650. DOI: 10.3390/ijms19030789. View

Liu Y, Tikunov Y, Schouten R, Marcelis L, Visser R, Bovy A . Anthocyanin Biosynthesis and Degradation Mechanisms in Vegetables: A Review. Front Chem. 2018; 6:52. PMC: 5855062. DOI: 10.3389/fchem.2018.00052. View

10.

Kumar S, Stecher G, Tamura K . MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016; 33(7):1870-4. PMC: 8210823. DOI: 10.1093/molbev/msw054. View

11.

Gurbuz N, Uluisik S, Frary A, Frary A, Doganlar S . Health benefits and bioactive compounds of eggplant. Food Chem. 2018; 268:602-610. DOI: 10.1016/j.foodchem.2018.06.093. View

12.

Hirakawa H, Shirasawa K, Miyatake K, Nunome T, Negoro S, Ohyama A . Draft genome sequence of eggplant (Solanum melongena L.): the representative solanum species indigenous to the old world. DNA Res. 2014; 21(6):649-60. PMC: 4263298. DOI: 10.1093/dnares/dsu027. View

13.

Laurentin A, Edwards C . A microtiter modification of the anthrone-sulfuric acid colorimetric assay for glucose-based carbohydrates. Anal Biochem. 2003; 315(1):143-5. DOI: 10.1016/s0003-2697(02)00704-2. View

14.

Benzie I, Strain J . The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1):70-6. DOI: 10.1006/abio.1996.0292. View

15.

Sievers F, Wilm A, Dineen D, Gibson T, Karplus K, Li W . Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol. 2011; 7:539. PMC: 3261699. DOI: 10.1038/msb.2011.75. View

16.

Docimo T, Francese G, Ruggiero A, Batelli G, De Palma M, Bassolino L . Phenylpropanoids Accumulation in Eggplant Fruit: Characterization of Biosynthetic Genes and Regulation by a MYB Transcription Factor. Front Plant Sci. 2016; 6:1233. PMC: 4729908. DOI: 10.3389/fpls.2015.01233. View

17.

Schilmiller A, Stout J, Weng J, Humphreys J, Ruegger M, Chapple C . Mutations in the cinnamate 4-hydroxylase gene impact metabolism, growth and development in Arabidopsis. Plant J. 2009; 60(5):771-82. DOI: 10.1111/j.1365-313X.2009.03996.x. View

18.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

19.

Huang D, Ou B, Prior R . The chemistry behind antioxidant capacity assays. J Agric Food Chem. 2005; 53(6):1841-56. DOI: 10.1021/jf030723c. View

20.

Kaushik P, Andujar I, Vilanova S, Plazas M, Gramazio P, Herraiz F . Breeding Vegetables with Increased Content in Bioactive Phenolic Acids. Molecules. 2015; 20(10):18464-81. PMC: 6332125. DOI: 10.3390/molecules201018464. View