Resveratrol Inhibits Proliferation and Differentiation of Porcine Preadipocytes by a Novel LincRNA-ROFM/miR-133b/AdipoQ Pathway

Overview

Journal Foods

Specialty Biotechnology

Date 2022 Sep 9

PMID 36076875

Authors

Qinyang Jiang

Sanbao Zhang

Xiaotong Gao

Yan Hu

Yu Zhang

Yujian Shen

Yuhang Jiang

Yanna Huang

Affiliations

Soon will be listed here.

Abstract

Resveratrol (RES) has a wide range of biological and pharmacological activities with various health benefits for humans as a food additive. In animal production, RES has been considered a potential functional feed additive for producing high-quality pork. Long noncoding RNAs (lncRNAs) have emerged as essential regulators of fat metabolism, and phytochemicals can regulate fat metabolism through lncRNA. However, it is unclear whether RES can improve back-fat thickness by regulating lncRNA. In this study, we identified a novel lncRNA, which was named a long intergenic non-protein coding RNA, a regulator of fat metabolism (), from our previous lncRNA sequencing data. can inhibit adipocyte proliferation and differentiation. In-depth analyses showed that acts as a molecular sponge for and adiponectin (AdipoQ) is a direct target of in porcine preadipocytes. In addition, the expression of was positively correlated with AdipoQ. RES can promote the expression of by α and α. Altogether, our research showed that acts as a ceRNA to sequester and is upregulated by RES, leading to heightened AdipoQ expression, and thus decreased adipocyte proliferation and differentiation, which reduces back-fat thickness of pigs. Taken together, the AdipoQ regulatory network preliminarily explains the mechanism of action of RES in inhibiting fat deposition, which provides new insight into the downstream mechanism of RES inhibition of fat deposits by regulating the lncRNA.

References

Juan M, Vinardell M, Planas J . The daily oral administration of high doses of trans-resveratrol to rats for 28 days is not harmful. J Nutr. 2002; 132(2):257-60. DOI: 10.1093/jn/132.2.257. View

Matsubara Y, Sato K, Ishii H, Akiba Y . Changes in mRNA expression of regulatory factors involved in adipocyte differentiation during fatty acid induced adipogenesis in chicken. Comp Biochem Physiol A Mol Integr Physiol. 2005; 141(1):108-15. DOI: 10.1016/j.cbpb.2005.04.013. View

Peng Y, Chen F, Ge J, Zhu J, Shi X, Li X . miR-429 Inhibits Differentiation and Promotes Proliferation in Porcine Preadipocytes. Int J Mol Sci. 2016; 17(12). PMC: 5187847. DOI: 10.3390/ijms17122047. View

Kumar A, Lawrence Jr J, Jung D, Ko H, Keller S, Kim J . Fat cell-specific ablation of rictor in mice impairs insulin-regulated fat cell and whole-body glucose and lipid metabolism. Diabetes. 2010; 59(6):1397-406. PMC: 2874700. DOI: 10.2337/db09-1061. View

Zhang L, Huang Y, Wang M, Guo Y, Liang J, Yang X . Development and Genome Sequencing of a Laboratory-Inbred Miniature Pig Facilitates Study of Human Diabetic Disease. iScience. 2019; 19:162-176. PMC: 6677790. DOI: 10.1016/j.isci.2019.07.025. View

Wang G, Wang Y, Xiong Y, Chen X, Ma M, Cai R . Sirt1 AS lncRNA interacts with its mRNA to inhibit muscle formation by attenuating function of miR-34a. Sci Rep. 2016; 6:21865. PMC: 4763196. DOI: 10.1038/srep21865. View

Chen L, Yang W, Guo Y, Chen W, Zheng P, Zeng J . Exosomal lncRNA GAS5 regulates the apoptosis of macrophages and vascular endothelial cells in atherosclerosis. PLoS One. 2017; 12(9):e0185406. PMC: 5612752. DOI: 10.1371/journal.pone.0185406. View

Kazimierczyk M, Kasprowicz M, Kasprzyk M, Wrzesinski J . Human Long Noncoding RNA Interactome: Detection, Characterization and Function. Int J Mol Sci. 2020; 21(3). PMC: 7037361. DOI: 10.3390/ijms21031027. View

Hsieh T, Wu J . Resveratrol: Biological and pharmaceutical properties as anticancer molecule. Biofactors. 2010; 36(5):360-9. PMC: 3655417. DOI: 10.1002/biof.105. View

10.

Liu L, He J, Xie H, Yang Y, Li J, Zou Y . Resveratrol induces antioxidant and heat shock protein mRNA expression in response to heat stress in black-boned chickens. Poult Sci. 2014; 93(1):54-62. DOI: 10.3382/ps.2013-03423. View

11.

Yu Q, Fang C, Ma Y, He S, Ajuwon K, He J . Dietary resveratrol supplement improves carcass traits and meat quality of Pekin ducks. Poult Sci. 2021; 100(3):100802. PMC: 7936143. DOI: 10.1016/j.psj.2020.10.056. View

12.

Miao Z, Wang S, Zhang J, Wei P, Guo L, Liu D . Identification and comparison of long non-conding RNA in Jinhua and Landrace pigs. Biochem Biophys Res Commun. 2018; 506(3):765-771. DOI: 10.1016/j.bbrc.2018.06.028. View

13.

Huang Y, Lang H, Chen K, Zhang Y, Gao Y, Ran L . Resveratrol protects against nonalcoholic fatty liver disease by improving lipid metabolism and redox homeostasis via the PPARα pathway. Appl Physiol Nutr Metab. 2019; 45(3):227-239. DOI: 10.1139/apnm-2019-0057. View

14.

Morlando M, Ballarino M, Fatica A . Long Non-Coding RNAs: New Players in Hematopoiesis and Leukemia. Front Med (Lausanne). 2015; 2:23. PMC: 4396502. DOI: 10.3389/fmed.2015.00023. View

15.

Font-I-Furnols M, Tous N, Esteve-Garcia E, Gispert M . Do all the consumers accept marbling in the same way? The relationship between eating and visual acceptability of pork with different intramuscular fat content. Meat Sci. 2012; 91(4):448-53. DOI: 10.1016/j.meatsci.2012.02.030. View

16.

Chi Khang Au P, Zhu Q, Dennis E, Wang M . Long non-coding RNA-mediated mechanisms independent of the RNAi pathway in animals and plants. RNA Biol. 2011; 8(3):404-14. DOI: 10.4161/rna.8.3.14382. View

17.

Ulitsky I, Bartel D . lincRNAs: genomics, evolution, and mechanisms. Cell. 2013; 154(1):26-46. PMC: 3924787. DOI: 10.1016/j.cell.2013.06.020. View

18.

de Ligt M, Timmers S, Schrauwen P . Resveratrol and obesity: Can resveratrol relieve metabolic disturbances?. Biochim Biophys Acta. 2014; 1852(6):1137-44. DOI: 10.1016/j.bbadis.2014.11.012. View

19.

Sakaue H, Konishi M, Ogawa W, Asaki T, Mori T, Yamasaki M . Requirement of fibroblast growth factor 10 in development of white adipose tissue. Genes Dev. 2002; 16(8):908-12. PMC: 152358. DOI: 10.1101/gad.983202. View

20.

Zhou S, Ding R, Meng F, Wang X, Zhuang Z, Quan J . A meta-analysis of genome-wide association studies for average daily gain and lean meat percentage in two Duroc pig populations. BMC Genomics. 2021; 22(1):12. PMC: 7788875. DOI: 10.1186/s12864-020-07288-1. View