De Novo Assembly and Annotation of the CHOZN® GS Genome Supports High-throughput Genome-scale Screening

Overview

Journal Biotechnol Bioeng

Publisher Wiley

Specialty Biochemistry

Date 2022 Sep 8

PMID 36073082

Authors

Corey Kretzmer

Rajagopalan Lakshmi Narasimhan

Rahul Deva Lal

Vincent Balassi

James Ravellette

Ajaya Kumar Kotekar Manjunath

Jesvin Joy Koshy

Marta Viano

Serena Torre

Valeria M Zanda

Mausam Kumravat

Keith Metelo Raul Saldanha

Harikrishnan Chandranpillai

Ifra Nihad

Fei Zhong

Yi Sun

Jason Gustin

Trissa Borgschulte

Jiajian Liu

David Razafsky

Affiliations

Soon will be listed here.

Abstract

Chinese hamster ovary (CHO) cells have been used as the industry standard for the production of therapeutic monoclonal antibodies for several decades. Despite significant improvements in commercial-scale production processes and media, the CHO cell has remained largely unchanged. Due to the cost and complexity of whole-genome sequencing and gene-editing it has been difficult to obtain the tools necessary to improve the CHO cell line. With the advent of next-generation sequencing and the discovery of the CRISPR/Cas9 system it has become more cost effective to sequence and manipulate the CHO genome. Here, we provide a comprehensive de novo assembly and annotation of the CHO-K1 based CHOZN® GS genome. Using this platform, we designed, built, and confirmed the functionality of a whole genome CRISPR guide RNA library that will allow the bioprocessing community to design a more robust CHO cell line leading to the production of life saving medications in a more cost-effective manner.

Citing Articles

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