Meta-Analysis of Mechanism of Influence of CRY2 on the Differentiation of Mouse Osteoblast Through the Regulation of Wnt/-Catenin Signaling Pathway

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2022 Sep 1

PMID 36046448

Authors

Chao Ma

Chaojian Yang

Tong Xie

Wenjuan Dai

Jun Ma

Affiliations

Soon will be listed here.

Abstract

Noncoding RNAs were discovered to control a variety of developmental mechanisms, including osteogenesis. According to emerging evidence, cryptochrome circadian-regulating (CRY) proteins have emerged as essential controllers of osteoblast differentiation. The linked processes, on the other hand, are still unknown. The specific process that underpins osteoblast differentiation and proliferation is yet unknown. This research gives a meta-analysis of CRY2's impact on mouse osteoblast differentiation via the control of the WNT/-catenin signaling pathways. Western blot and quantitative real-time PCR were used to identify Cry2 expression levels, components in the osteoblast-associated signaling pathway, and osteoblast transcription markers. The osteogenic condition was measured utilizing alkaline phosphatase (ALP) and alizarin red (AR) staining, whereas cell growth rates were measured using CCK8 assays. An ectopic bone formation experiment was used to determine the osteogenic potential of osteoblasts. Cry2 stimulates the osteogenic development of mouse osteoblasts through canonical Wnt/-catenin signaling, according to the findings.

Citing Articles

Retracted: Meta-Analysis of Mechanism of Influence of CRY2 on the Differentiation of Mouse Osteoblast through the Regulation of Wnt/-Catenin Signaling Pathway.

International B Biomed Res Int. 2024; 2024:9873941.

PMID: 38230196 PMC: 10791321. DOI: 10.1155/2024/9873941.

References

Zhang Y, Gu X, Li D, Cai L, Xu Q . METTL3 Regulates Osteoblast Differentiation and Inflammatory Response via Smad Signaling and MAPK Signaling. Int J Mol Sci. 2020; 21(1). PMC: 6981640. DOI: 10.3390/ijms21010199. View

Tang Z, Xu T, Li Y, Fei W, Yang G, Hong Y . Inhibition of CRY2 by STAT3/miRNA-7-5p Promotes Osteoblast Differentiation through Upregulation of CLOCK/BMAL1/P300 Expression. Mol Ther Nucleic Acids. 2020; 19:865-876. PMC: 6994415. DOI: 10.1016/j.omtn.2019.12.020. View

Tsang K, Liu H, Yang Y, Charles J, Ermann J . Defective circadian control in mesenchymal cells reduces adult bone mass in mice by promoting osteoclast function. Bone. 2019; 121:172-180. PMC: 6699618. DOI: 10.1016/j.bone.2019.01.016. View

Wu Q, Wang J, Tong X, Chen J, Wang B, Miao Z . Emerging role of circadian rhythm in bone remodeling. J Mol Med (Berl). 2018; 97(1):19-24. DOI: 10.1007/s00109-018-1723-9. View

Gomathi K, Akshaya N, Srinaath N, Moorthi A, Selvamurugan N . Regulation of Runx2 by post-translational modifications in osteoblast differentiation. Life Sci. 2020; 245:117389. DOI: 10.1016/j.lfs.2020.117389. View

Kim K, Kim J, Kim I, Seong S, Kim N . Rev-erbα Negatively Regulates Osteoclast and Osteoblast Differentiation through p38 MAPK Signaling Pathway. Mol Cells. 2020; 43(1):34-47. PMC: 6999712. DOI: 10.14348/molcells.2019.0232. View

Liu B, Lu Y, Wang Y, Ge L, Zhai N, Han J . A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria. Cell Tissue Bank. 2019; 20(2):173-182. PMC: 6556159. DOI: 10.1007/s10561-019-09751-0. View

Yin C, Tian Y, Yu Y, Wang H, Wu Z, Huang Z . A novel long noncoding RNA AK016739 inhibits osteoblast differentiation and bone formation. J Cell Physiol. 2019; 234(7):11524-11536. DOI: 10.1002/jcp.27815. View

Mammoli F, Castiglioni S, Parenti S, Cappadone C, Farruggia G, Iotti S . Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D₃. Int J Mol Sci. 2019; 20(2). PMC: 6358963. DOI: 10.3390/ijms20020385. View

10.

Garg P, Mazur M, Buck A, Wandtke M, Liu J, Ebraheim N . Prospective Review of Mesenchymal Stem Cells Differentiation into Osteoblasts. Orthop Surg. 2017; 9(1):13-19. PMC: 6584428. DOI: 10.1111/os.12304. View

11.

Karvande A, Kushwaha P, Ahmad N, Adhikary S, Kothari P, Tripathi A . Glucose dependent miR-451a expression contributes to parathyroid hormone mediated osteoblast differentiation. Bone. 2018; 117:98-115. DOI: 10.1016/j.bone.2018.09.007. View

12.

Hirai T . Regulation of Clock Genes by Adrenergic Receptor Signaling in Osteoblasts. Neurochem Res. 2017; 43(1):129-135. DOI: 10.1007/s11064-017-2365-y. View

13.

Paine A, Woeller C, Zhang H, Garcia-Hernandez M, Huertas N, Xing L . Thy1 is a positive regulator of osteoblast differentiation and modulates bone homeostasis in obese mice. FASEB J. 2018; 32(6):3174-3183. PMC: 5956243. DOI: 10.1096/fj.201701379R. View

14.

Chan W, Tan Z, To M, Chan D . Regulation and Role of Transcription Factors in Osteogenesis. Int J Mol Sci. 2021; 22(11). PMC: 8196788. DOI: 10.3390/ijms22115445. View

15.

Zhuo H, Wang Y, Zhao Q . The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation. Stem Cells Int. 2018; 2018:3407821. PMC: 5896276. DOI: 10.1155/2018/3407821. View

16.

Takarada T, Xu C, Ochi H, Nakazato R, Yamada D, Nakamura S . Bone Resorption Is Regulated by Circadian Clock in Osteoblasts. J Bone Miner Res. 2016; 32(4):872-881. DOI: 10.1002/jbmr.3053. View

17.

Bao Q, Chen S, Qin H, Feng J, Liu H, Liu D . Constitutive β-catenin activation in osteoblasts impairs terminal osteoblast differentiation and bone quality. Exp Cell Res. 2016; 350(1):123-131. DOI: 10.1016/j.yexcr.2016.11.013. View

18.

Narayanan A, Srinaath N, Rohini M, Selvamurugan N . Regulation of Runx2 by MicroRNAs in osteoblast differentiation. Life Sci. 2019; 232:116676. DOI: 10.1016/j.lfs.2019.116676. View

19.

Luo B, Zhou X, Tang Q, Yin Y, Feng G, Li S . Circadian rhythms affect bone reconstruction by regulating bone energy metabolism. J Transl Med. 2021; 19(1):410. PMC: 8477514. DOI: 10.1186/s12967-021-03068-x. View

20.

Zheng J, Zhang L, Tan Z, Zhao Q, Wei X, Yang Y . Bmal1- and Per2-mediated regulation of the osteogenic differentiation and proliferation of mouse bone marrow mesenchymal stem cells by modulating the Wnt/β-catenin pathway. Mol Biol Rep. 2022; 49(6):4485-4501. DOI: 10.1007/s11033-022-07292-6. View