Prospective Review of Mesenchymal Stem Cells Differentiation into Osteoblasts

Overview

Journal Orthop Surg

Specialty Orthopedics

Date 2017 Mar 10

PMID 28276640

Citations 84

Authors

Priyanka Garg

Matthew M Mazur

Amy C Buck

Meghan E Wandtke

Jiayong Liu

Nabil A Ebraheim

Affiliations

Soon will be listed here.

Abstract

Stem cell research has been a popular topic in the past few decades. This review aims to discuss factors that help regulate, induce, and enhance mesenchymal stem cell (MSC) differentiation into osteoblasts for bone regeneration. The factors analyzed include bone morphogenic protein (BMP), transforming growth factor β (TGF-β), stromal cell-derived factor 1 (SDF-1), insulin-like growth factor type 1 (IGF-1), histone demethylase JMJD3, cyclin dependent kinase 1 (CDK1), fucoidan, Runx2 transcription factor, and TAZ transcriptional coactivator. Methods promoting bone healing are also evaluated in this review that have shown promise in previous studies. Methods tested using animal models include low intensity pulsed ultrasound (LIPUS) with MSC, micro motion, AMD3100 injections, BMP delivery, MSC transplantation, tissue engineering utilizing scaffolds, anti-IL-20 monoclonal antibody, low dose photodynamic therapy, and bone marrow stromal cell transplants. Human clinical trial methods analyzed include osteoblast injections, bone marrow grafts, bone marrow and platelet rich plasma transplantation, tissue engineering using scaffolds, and recombinant human BMP-2. These methods have been shown to promote and accelerate new bone formation. These various methods for enhanced bone regeneration have the potential to be used, following further research, in clinical practice.

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References

Jaiswal R, Jaiswal N, Bruder S, Mbalaviele G, Marshak D, Pittenger M . Adult human mesenchymal stem cell differentiation to the osteogenic or adipogenic lineage is regulated by mitogen-activated protein kinase. J Biol Chem. 2000; 275(13):9645-52. DOI: 10.1074/jbc.275.13.9645. View

Shen F, Visger J, Balian G, Hurwitz S, Diduch D . Systemically administered mesenchymal stromal cells transduced with insulin-like growth factor-I localize to a fracture site and potentiate healing. J Orthop Trauma. 2002; 16(9):651-9. DOI: 10.1097/00005131-200210000-00007. View

Thompson Z, Miclau T, Hu D, Helms J . A model for intramembranous ossification during fracture healing. J Orthop Res. 2002; 20(5):1091-8. DOI: 10.1016/S0736-0266(02)00017-7. View

Govender S, Csimma C, Genant H, Valentin-Opran A, Amit Y, Arbel R . Recombinant human bone morphogenetic protein-2 for treatment of open tibial fractures: a prospective, controlled, randomized study of four hundred and fifty patients. J Bone Joint Surg Am. 2002; 84(12):2123-34. DOI: 10.2106/00004623-200212000-00001. View

Franceschi R, Xiao G . Regulation of the osteoblast-specific transcription factor, Runx2: responsiveness to multiple signal transduction pathways. J Cell Biochem. 2003; 88(3):446-54. DOI: 10.1002/jcb.10369. View

Cheng H, Jiang W, Phillips F, Haydon R, Peng Y, Zhou L . Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Joint Surg Am. 2003; 85(8):1544-52. DOI: 10.2106/00004623-200308000-00017. View

Pelissier P, Masquelet A, Bareille R, Pelissier S, Amedee J . Induced membranes secrete growth factors including vascular and osteoinductive factors and could stimulate bone regeneration. J Orthop Res. 2003; 22(1):73-9. DOI: 10.1016/S0736-0266(03)00165-7. View

Wynn R, Hart C, Corradi-Perini C, ONeill L, Evans C, Wraith J . A small proportion of mesenchymal stem cells strongly expresses functionally active CXCR4 receptor capable of promoting migration to bone marrow. Blood. 2004; 104(9):2643-5. DOI: 10.1182/blood-2004-02-0526. View

Simmons C, Alsberg E, Hsiong S, Kim W, Mooney D . Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells. Bone. 2004; 35(2):562-9. DOI: 10.1016/j.bone.2004.02.027. View

10.

Peterson B, Zhang J, Iglesias R, Kabo M, Hedrick M, Benhaim P . Healing of critically sized femoral defects, using genetically modified mesenchymal stem cells from human adipose tissue. Tissue Eng. 2005; 11(1-2):120-9. DOI: 10.1089/ten.2005.11.120. View

11.

Kratchmarova I, Blagoev B, Haack-Sorensen M, Kassem M, Mann M . Mechanism of divergent growth factor effects in mesenchymal stem cell differentiation. Science. 2005; 308(5727):1472-7. DOI: 10.1126/science.1107627. View

12.

Hernigou P, Poignard A, Beaujean F, Rouard H . Percutaneous autologous bone-marrow grafting for nonunions. Influence of the number and concentration of progenitor cells. J Bone Joint Surg Am. 2005; 87(7):1430-7. DOI: 10.2106/JBJS.D.02215. View

13.

Hong J, Hwang E, McManus M, Amsterdam A, Tian Y, Kalmukova R . TAZ, a transcriptional modulator of mesenchymal stem cell differentiation. Science. 2005; 309(5737):1074-8. DOI: 10.1126/science.1110955. View

14.

Dimitriou R, Tsiridis E, Giannoudis P . Current concepts of molecular aspects of bone healing. Injury. 2005; 36(12):1392-404. DOI: 10.1016/j.injury.2005.07.019. View

15.

Hong J, Yaffe M . TAZ: a beta-catenin-like molecule that regulates mesenchymal stem cell differentiation. Cell Cycle. 2006; 5(2):176-9. DOI: 10.4161/cc.5.2.2362. View

16.

Koch H, Jadlowiec J, Campbell P . Insulin-like growth factor-I induces early osteoblast gene expression in human mesenchymal stem cells. Stem Cells Dev. 2006; 14(6):621-31. DOI: 10.1089/scd.2005.14.621. View

17.

Mankani M, Kuznetsov S, Shannon B, Nalla R, Ritchie R, Qin Y . Canine cranial reconstruction using autologous bone marrow stromal cells. Am J Pathol. 2006; 168(2):542-50. PMC: 1606510. DOI: 10.2353/ajpath.2006.050407. View

18.

Komori T . Regulation of osteoblast differentiation by transcription factors. J Cell Biochem. 2006; 99(5):1233-9. DOI: 10.1002/jcb.20958. View

19.

Jones A, Bucholz R, Bosse M, Mirza S, Lyon T, Webb L . Recombinant human BMP-2 and allograft compared with autogenous bone graft for reconstruction of diaphyseal tibial fractures with cortical defects. A randomized, controlled trial. J Bone Joint Surg Am. 2006; 88(7):1431-41. DOI: 10.2106/JBJS.E.00381. View

20.

Takahashi K, Yamanaka S . Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-76. DOI: 10.1016/j.cell.2006.07.024. View