Fiber Photometry in Striatum Reflects Primarily Nonsomatic Changes in Calcium

Overview

Journal Nat Neurosci

Date 2022 Aug 30

PMID 36042311

Authors

Alex A Legaria

Bridget A Matikainen-Ankney

Ben Yang

Biafra Ahanonu

Julia A Licholai

Jones G Parker

Alexxai V Kravitz

Affiliations

Soon will be listed here.

Abstract

Fiber photometry enables recording of population neuronal calcium dynamics in awake mice. While the popularity of fiber photometry has grown in recent years, it remains unclear whether photometry reflects changes in action potential firing (that is, 'spiking') or other changes in neuronal calcium. In microscope-based calcium imaging, optical and analytical approaches can help differentiate somatic from neuropil calcium. However, these approaches cannot be readily applied to fiber photometry. As such, it remains unclear whether the fiber photometry signal reflects changes in somatic calcium, changes in nonsomatic calcium or a combination of the two. Here, using simultaneous in vivo extracellular electrophysiology and fiber photometry, along with in vivo endoscopic one-photon and two-photon calcium imaging, we determined that the striatal fiber photometry does not reflect spiking-related changes in calcium and instead primarily reflects nonsomatic changes in calcium.

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