Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with FRT10

Overview

Journal Foods

Specialty Biotechnology

Date 2022 Aug 26

PMID 36010491

Authors

Hongying Cai

Daojie Li

Liye Song

Xin Xu

Yunsheng Han

Kun Meng

Zhiguo Wen

Peilong Yang

Affiliations

Soon will be listed here.

Abstract

Obesity has become a major social problem related to health and quality of life. Our previous work demonstrated that FRT10 alleviated obesity in high-fat diet (HFD)-fed mice by alleviating gut dysbiosis. However, the underlying functions of FRT10 in regulating liver and cecum contents metabolism remain unknown. Liver and cecum contents metabonomics combined with pathway analysis based on ultraperformance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) were performed to evaluate the alterations of metabolic profiles between obese control mice and obese mice in FRT10-treated groups. The orthogonal partial least squares discriminant analysis (OPLS-DA) score plots showed that there were significant differences in cecum contents and liver markers between experimental groups. In total, 26 potential biomarkers were identified in the liver and 15 in cecum contents that could explain the effect of FRT10 addition in HFD-fed mice. In addition, gut-liver axis analysis indicated that there was a strong correlation between cecum contents metabolites and hepatic metabolites. The mechanism of FRT10 against obesity might be related to the alterations in glycerophospholipid metabolism, primary bile acid biosynthesis, amino metabolism, and purine and pyrimidine metabolism. Studies on these metabolites could help us better understand the role of FRT10 in obesity induced by HFD.

Citing Articles

Dietary fiber content in clinical ketogenic diets modifies the gut microbiome and seizure resistance in mice.

Ozcan E, Yu K, Dinh L, Lum G, Lau K, Hsu J Nat Commun. 2025; 16(1):987.

PMID: 39856104 PMC: 11759687. DOI: 10.1038/s41467-025-56091-7.

References

Johnson C, Ivanisevic J, Siuzdak G . Metabolomics: beyond biomarkers and towards mechanisms. Nat Rev Mol Cell Biol. 2016; 17(7):451-9. PMC: 5729912. DOI: 10.1038/nrm.2016.25. View

Yin X, Peng J, Zhao L, Yu Y, Zhang X, Liu P . Structural changes of gut microbiota in a rat non-alcoholic fatty liver disease model treated with a Chinese herbal formula. Syst Appl Microbiol. 2013; 36(3):188-96. DOI: 10.1016/j.syapm.2012.12.009. View

Li X, Xiao Y, Song L, Huang Y, Chu Q, Zhu S . Effect of Lactobacillus plantarum HT121 on serum lipid profile, gut microbiota, and liver transcriptome and metabolomics in a high-cholesterol diet-induced hypercholesterolemia rat model. Nutrition. 2020; 79-80:110966. DOI: 10.1016/j.nut.2020.110966. View

Wu H, Xie S, Miao J, Li Y, Wang Z, Wang M . maintains intestinal epithelial regeneration and repairs damaged intestinal mucosa. Gut Microbes. 2020; 11(4):997-1014. PMC: 7524370. DOI: 10.1080/19490976.2020.1734423. View

An Y, Xu W, Li H, Lei H, Zhang L, Hao F . High-fat diet induces dynamic metabolic alterations in multiple biological matrices of rats. J Proteome Res. 2013; 12(8):3755-68. DOI: 10.1021/pr400398b. View

Kim H, Kim J, Noh S, Hur H, Sung M, Hwang J . Metabolomic analysis of livers and serum from high-fat diet induced obese mice. J Proteome Res. 2010; 10(2):722-31. DOI: 10.1021/pr100892r. View

Park H, Park K, Park E, Kim S, Choi M, Liu K . Mass Spectrometry-Based Metabolomic and Lipidomic Analyses of the Effects of Dietary Platycodon grandiflorum on Liver and Serum of Obese Mice under a High-Fat Diet. Nutrients. 2017; 9(1). PMC: 5295115. DOI: 10.3390/nu9010071. View

Hussain A, Kwon M, Kim H, Lee H, Cho J, Lee Y . Anti-Obesity Effect of LB818 Is Associated with Regulation of Gut Microbiota in High-Fat Diet-Fed Obese Mice. J Med Food. 2020; 23(7):750-759. DOI: 10.1089/jmf.2019.4627. View

Bashiardes S, Shapiro H, Rozin S, Shibolet O, Elinav E . Non-alcoholic fatty liver and the gut microbiota. Mol Metab. 2016; 5(9):782-94. PMC: 5004228. DOI: 10.1016/j.molmet.2016.06.003. View

10.

Choi W, Dong H, Jeong H, Ryu D, Song S, Kim Y . Lactobacillus plantarum LMT1-48 exerts anti-obesity effect in high-fat diet-induced obese mice by regulating expression of lipogenic genes. Sci Rep. 2020; 10(1):869. PMC: 6972779. DOI: 10.1038/s41598-020-57615-5. View

11.

Zhao Y, Miao H, Cheng X, Wei F . Lipidomics: Novel insight into the biochemical mechanism of lipid metabolism and dysregulation-associated disease. Chem Biol Interact. 2015; 240:220-38. DOI: 10.1016/j.cbi.2015.09.005. View

12.

Porras D, Nistal E, Martinez-Florez S, Gonzalez-Gallego J, Garcia-Mediavilla M, Sanchez-Campos S . Intestinal Microbiota Modulation in Obesity-Related Non-alcoholic Fatty Liver Disease. Front Physiol. 2019; 9:1813. PMC: 6305464. DOI: 10.3389/fphys.2018.01813. View

13.

Kennedy M, Moffat T, Gable K, Ganesan S, Niewola-Staszkowska K, Johnston A . A Signaling Lipid Associated with Alzheimer's Disease Promotes Mitochondrial Dysfunction. Sci Rep. 2016; 6:19332. PMC: 4725818. DOI: 10.1038/srep19332. View

14.

Brkic L, Riederer M, Graier W, Malli R, Frank S . Acyl chain-dependent effect of lysophosphatidylcholine on cyclooxygenase (COX)-2 expression in endothelial cells. Atherosclerosis. 2012; 224(2):348-54. PMC: 3465554. DOI: 10.1016/j.atherosclerosis.2012.07.038. View

15.

Kim M, Kim I, Sung H, Nam M, Kim Y, Kyung D . Metabolic dysfunction following weight regain compared to initial weight gain in a high-fat diet-induced obese mouse model. J Nutr Biochem. 2019; 69:44-52. DOI: 10.1016/j.jnutbio.2019.02.011. View

16.

Martinic A, Barouei J, Bendiks Z, Mishchuk D, Heeney D, Martin R . Supplementation of Lactobacillus plantarum Improves Markers of Metabolic Dysfunction Induced by a High Fat Diet. J Proteome Res. 2018; 17(8):2790-2802. DOI: 10.1021/acs.jproteome.8b00282. View

17.

Saiki S, Sato T, Kohzuki M, Kamimoto M, Yosida T . Changes in serum hypoxanthine levels by exercise in obese subjects. Metabolism. 2001; 50(6):627-30. DOI: 10.1053/meta.2001.24197. View

18.

Furuhashi M, Koyama M, Higashiura Y, Murase T, Nakamura T, Matsumoto M . Differential regulation of hypoxanthine and xanthine by obesity in a general population. J Diabetes Investig. 2020; 11(4):878-887. PMC: 7378426. DOI: 10.1111/jdi.13207. View

19.

Liu Y, Xie C, Zhai Z, Deng Z, De Jonge H, Wu X . Uridine attenuates obesity, ameliorates hepatic lipid accumulation and modifies the gut microbiota composition in mice fed with a high-fat diet. Food Funct. 2021; 12(4):1829-1840. DOI: 10.1039/d0fo02533j. View

20.

Nakamura T, Nampei M, Murase T, Satoh E, Akari S, Katoh N . Influence of xanthine oxidoreductase inhibitor, topiroxostat, on body weight of diabetic obese mice. Nutr Diabetes. 2021; 11(1):12. PMC: 8044114. DOI: 10.1038/s41387-021-00155-2. View