Effects of Ciprofloxacin Alone or in Mixture with Sulfamethoxazole on the Efficiency of Anaerobic Digestion and Its Microbial Community

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2022 Aug 26

PMID 36009981

Authors

Valentina Mazzurco Miritana

Luisa Patrolecco

Anna Barra Caracciolo

Andrea Visca

Flavia Piccinini

Antonella Signorini

Silvia Rosa

Paola Grenni

Gian Luigi Garbini

Francesca Spataro

Jasmin Rauseo

Giulia Massini

Affiliations

Soon will be listed here.

Abstract

Some livestock farms rely on anaerobic digestion (AD) technology for manure disposal, thus obtaining energy (biogas) and fertilizer (digestate). Mixtures of antibiotics used for animal health often occur in organic waste and their possible synergistic/antagonistic effects on microorganisms involved in AD are still poorly studied. This work focuses on the effects of adding ciprofloxacin, alone (5 mg L) and in combination with sulfamethoxazole (2.5-5-10 mg L), on AD efficiency and microbial community structure. The experiment consisted of 90-day cattle manure batch tests and antibiotic removal percentages were assessed. Adding antibiotics always promoted CH and H production compared to untreated controls; however, CH production was lowered with the highest ciprofloxacin (CIP) concentrations. The overall results show antibiotic degradation caused by acidogenic , and CH was mainly produced through the hydrogenotrophic-pathway by methanogenic . Shifts in microbial community abundance (DAPI counts) and composition (Illumina-MiSeq and FISH analyses) were observed.

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