Genetic Analysis of Two Genes, DnaJ and DnaK, Necessary for Escherichia Coli and Bacteriophage Lambda DNA Replication

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1978 Aug 4

PMID 360041

Citations 69

Authors

J Yochem

H Uchida

M Sunshine

H Saito

C P Georgopoulos

M Feiss

Affiliations

Soon will be listed here.

Abstract

We show that a collection of 93 E. coli mutations which map between thr and leu and which block phage lambda DNA replication define two closely linked cistrons. Work published in the accompanying paper shows that these mutations also affect host DNA replication, so we designate them dnaJ and dnaK; the gene order is thr--dnaK--dnaJ--leu. Demonstration of two cistrons was possible with the isolation of lambda transducing phages carrying one or the other or both of the dna genes. These phages were employed in phage vs bacterial complementation studies which unambiguously show that dnaK and dnaJ are different cistrons.

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