A Ratiometric Fluorescent Nanoprobe for Ultrafast Imaging of Peroxynitrite in Living Cells

Overview

Journal J Biol Inorg Chem

Publisher Springer

Specialty Biochemistry

Date 2022 Aug 17

PMID 35976437

Authors

Yijing Cui

Suping Han

Jingjing Zhang

Xiaoqing Wang

Affiliations

Soon will be listed here.

Abstract

For ratiometrically imaging peroxynitrite (ONOO) in living cells, we devised and fabricated a novel fluorescent nanoprobe, NC-NP, in this study. To achieve ratiometric fluorescence response towards ONOO, NC-NP used 3-(2-benzothiazolyl) coumarin (Cou-Bz) as the internal reference and 1,8-naphthimide derivative (Naph-PN) as a fluorescent ONOO probe. These compounds were incorporated into an amphiphilic block polymer called Pluronic F-127. In addition to an ultrafast response to ONOO, NC-NP also showed great selectivity and sensitive detection (detection limit was 4.51 μM). It was important to note that NC-NP demonstrated solid performance for ONOO fluorescence ratio imaging in living cells, highlighting its potential for ONOO-related chemical biology research.

Citing Articles

Role of Peroxynitrite in the Pathogenesis of Parkinson's Disease and Its Fluorescence Imaging-Based Detection.

Lv J, Chen F, Zhang C, Kang Y, Yang Y, Zhang C Biosensors (Basel). 2024; 14(10).

PMID: 39451719 PMC: 11506598. DOI: 10.3390/bios14100506.

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