Macrophage Stimulation by Bacterial Lipopolysaccharides. II. Evidence for Differentiation Signals Delivered by Lipid A and by a Protein Rich Fraction of Lipopolysaccharides

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1978 Aug 1

PMID 359747

Citations 20

Authors

W F Doe

S T Yang

D C Morrison

S J Betz

P M Henson

Affiliations

Soon will be listed here.

Abstract

Stimulation of macrophages to lyse tumor cells is a property common to lipopolysaccharide (LPS) extracted from a variety of smooth and rough bacterial strains by several different preparative procedures. The relationship between macrophage stimulation and the structural characteristics of LPS is defined. In protein-free LPS, lipid A bears the stimulatory signal which results in the differentiation of elicited macrophages into killer cells. The polysaccharide moiety is neither stimulatory itself nor does it block the activity of complete LPS on macrophages. Extraction of LPS by the butanol or Boivin procedures produces preparations in which LPS is complexed through its lipid A moiety to a protein rich component, LAP. Isolated LAP delivers a macrophage differentiation signal which is independent of lipid A. The presence of these two structurally distinct constituents in the cell walls of gram-negative bacteria broadens the biological environments in which they can stimulate macrophages in vivo.

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References

Anacker R, Finkelstein R, HASKINS W, Landy M, MILNER K, Ribi E . ORIGIN AND PROPERTIES OF NATURALLY OCCURRING HAPTEN FROM ESCHERICHIA COLI. J Bacteriol. 1964; 88:1705-20. PMC: 277477. DOI: 10.1128/jb.88.6.1705-1720.1964. View

Koenig S, Hoffmann M, Thomas L . Induction of phenotypic lymphocyte differentiation in LPS unresponsive mice by an LPS-induced serum factor and by lipid A-associated protein. J Immunol. 1977; 118(5):1910-1. View

Morrison D, Jacobs D . Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry. 1976; 13(10):813-8. DOI: 10.1016/0019-2791(76)90181-6. View

Betz S, Morrison D . Chemical and biologic properties of a protein-rich fraction of bacterial lipopolysaccharides. I. The in vitro murine lymphocyte response. J Immunol. 1977; 119(4):1475-81. View

Morrison D, Kline L . Activation of the classical and properdin pathways of complement by bacterial lipopolysaccharides (LPS). J Immunol. 1977; 118(1):362-8. View

Watson J . Differentiation of B lymphocytes in C3H/HeJ mice: the induction of Ia antigens by lipopolysaccharide. J Immunol. 1977; 118(3):1103-8. View

Doe W, Henson P . Macrophage stimulation by bacterial lipopolysaccharides. I. Cytolytic effect on tumor target cells. J Exp Med. 1978; 148(2):544-56. PMC: 2184936. DOI: 10.1084/jem.148.2.544. View

Morrison D, Betz S, Jacobs D . Isolation of a lipid A bound polypeptide responsible for "LPS-initiated" mitogenesis of C3H/HeJ spleen cells. J Exp Med. 1976; 144(3):840-6. PMC: 2190404. DOI: 10.1084/jem.144.3.840. View

Morrison D, Cochrane C . Direct evidence for Hageman factor (factor XII) activation by bacterial lipopolysaccharides (endotoxins). J Exp Med. 1974; 140(3):797-811. PMC: 2139619. DOI: 10.1084/jem.140.3.797. View

10.

Niwa M, MILNER K, Ribi E, Rudbach J . Alteration of physical, chemical, and biological properties of endotoxin by treatment with mild alkali. J Bacteriol. 1969; 97(3):1069-77. PMC: 249816. DOI: 10.1128/jb.97.3.1069-1077.1969. View

11.

Alexander P, Evans R . Endotoxin and double stranded RNA render macrophages cytotoxic. Nat New Biol. 1971; 232(29):76-8. DOI: 10.1038/newbio232076a0. View

12.

Gewurz H, Shin H, Mergenhagen S . Interactions of the complement system with endotoxic lipopolysaccharide: consumption of each of the six terminal complement components. J Exp Med. 1968; 128(5):1049-57. PMC: 2138564. DOI: 10.1084/jem.128.5.1049. View

13.

Morrison D, Leive L . Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures. J Biol Chem. 1975; 250(8):2911-19. View

14.

Von Eschen K, Rudbach J . Immunological responses of mice to native protoplasmic polysaccharide and lipopolysaccharide: functional separation of the two signals required to stimulate a secondary antibody response. J Exp Med. 1974; 140(6):1604-14. PMC: 2139752. DOI: 10.1084/jem.140.6.1604. View

15.

Braun V . Molecular organization of the rigid layer and the cell wall of Escherichia coli. J Infect Dis. 1973; 128:Suppl:9-16. DOI: 10.1093/infdis/128.supplement_1.s9. View

16.

Loos M, Bitter-Suermann D, Dierich M . Interaction of the first (C1), the second (C2) and the fourth (C4) component of complement with different preparations of bacterial lipopolysaccharides and with lipid A. J Immunol. 1974; 112(3):935-40. View

17.

Andersson J, Moller G, Sjoberg O . Selective induction of DNA synthesis in T and B lymphocytes. Cell Immunol. 1972; 4(4):381-93. DOI: 10.1016/0008-8749(72)90040-8. View

18.

JACOBS M, Morrison D . Dissociation between mitogenicity and immunogenicity of TNP-lipopolysaccharide, a T-independent antigen. J Exp Med. 1975; 141(6):1453-8. PMC: 2189863. DOI: 10.1084/jem.141.6.1453. View

19.

Morrison D, Jacobs D . Inhibition of lipopolysaccharide-initiated activation of serum complement by polymyxin B. Infect Immun. 1976; 13(1):298-301. PMC: 420610. DOI: 10.1128/iai.13.1.298-301.1976. View

20.

Evans R . Macrophages and the tumour bearing host. Br J Cancer Suppl. 1973; 1:19-25. PMC: 2149057. View