Accuracy of Reverse-transcription Polymerase Chain Reaction and Loop-mediated Isothermal Amplification in Diagnosing Severe Fever with Thrombocytopenia Syndrome: A Systematic Review and Meta-analysis

Overview

Journal J Med Virol

Specialty Microbiology

Date 2022 Aug 15

PMID 35968756

Authors

Wen Tian

Xingxiang Ren

Xu Gao

Yuanyuan Zhang

Zhihai Chen

Wei Zhang

Affiliations

Soon will be listed here.

Abstract

Nucleic acid molecular diagnostic technology plays an important role in the detection of severe fever with thrombocytopenia syndrome (SFTS). However, no relevant reports have been published on the accuracy of reverse-transcription polymerase chain reaction (RT-PCR) and reverse-transcription loop-mediated isothermal amplification (RT-LAMP) in the diagnosis of SFTS. Thus, we conducted a meta-analysis and systematic review to evaluate the accuracy of the two methods. On June 19, 2022, we comprehensively searched the PubMed, Embase, Cochrane Library, Web of Science, Scoups, Ovid, Proquest, China National Knowledge Infrastructure Database, Wan Fang Data, Traditional Chinese Medicine Database (Sinomed), VIP Database, and Reading Showing Database for articles on nucleic acid diagnostic techniques, such as RT-PCR and RT-LAMP, used to diagnose SFTS. Statistical analysis was performed using STATA 14.0 and Meta-Disc 1.4. Sixteen articles involving 2942 clinical blood samples were included in the analysis. RT-PCR and RT-LAMP were used as index tests, whereas RT-PCR or other detection methods were used as reference standards. The pooled values for the sensitivity, specificity, positive and negative likelihood ratios of the RT-PCR test were 0.97 (95% confidence interval [CI]: 0.92-0.99), 1.00 (95% CI: 0.98-1.00), 483.87 (95% CI: 58.04-4033.76), and 0.03 (95% CI:0.01-0.08), respectively. Those for the RT-LAMP test were 0.95 (95% CI: 0.91-0.97), 0.99 (95% CI: 0.93-1.00), 111.18 (95% CI: 13.96-885.27), and 0.05 (95% CI: 0.03-0.09), respectively. Both RT-PCR and RT-LAMP have high diagnostic value in SFTS and can be applied in different scenarios for laboratory confirmation or on-site screening.

Citing Articles

Advancements in the Worldwide Detection of Severe Fever with Thrombocytopenia Syndrome Virus Infection from 2009 to 2023.

Ai L, Wang W, Teng Z China CDC Wkly. 2023; 5(31):687-693.

PMID: 37593140 PMC: 10427339. DOI: 10.46234/ccdcw2023.132.

Accuracy of reverse-transcription polymerase chain reaction and loop-mediated isothermal amplification in diagnosing severe fever with thrombocytopenia syndrome: A systematic review and meta-analysis.

Tian W, Ren X, Gao X, Zhang Y, Chen Z, Zhang W J Med Virol. 2022; 94(12):5922-5932.

PMID: 35968756 PMC: 9804528. DOI: 10.1002/jmv.28068.

References

Takayama-Ito M, Saijo M . Antiviral Drugs Against Severe Fever With Thrombocytopenia Syndrome Virus Infection. Front Microbiol. 2020; 11:150. PMC: 7026129. DOI: 10.3389/fmicb.2020.00150. View

Huang X, Hu X, Ma H, Du Y, Ma H, Kang K . Detection of new bunyavirus RNA by reverse transcription-loop-mediated isothermal amplification. J Clin Microbiol. 2014; 52(2):531-5. PMC: 3911317. DOI: 10.1128/JCM.01813-13. View

Gong L, Song D, Wu J, Cao M, Su B, Sun Y . Human-to-human transmissions of severe fever with thrombocytopenia syndrome virus in Anhui province, 2010-2017. Clin Microbiol Infect. 2018; 24(8):920-922. DOI: 10.1016/j.cmi.2018.03.014. View

Whiting P, Rutjes A, Westwood M, Mallett S, Deeks J, Reitsma J . QUADAS-2: a revised tool for the quality assessment of diagnostic accuracy studies. Ann Intern Med. 2011; 155(8):529-36. DOI: 10.7326/0003-4819-155-8-201110180-00009. View

Tian W, Ren X, Gao X, Zhang Y, Chen Z, Zhang W . Accuracy of reverse-transcription polymerase chain reaction and loop-mediated isothermal amplification in diagnosing severe fever with thrombocytopenia syndrome: A systematic review and meta-analysis. J Med Virol. 2022; 94(12):5922-5932. PMC: 9804528. DOI: 10.1002/jmv.28068. View

Huang X, Liu L, Du Y, Ma H, Mu Y, Wang P . Detection of a novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome in Henan Province, China, using real-time reverse transcription PCR. J Med Microbiol. 2013; 62(Pt 7):1060-1064. DOI: 10.1099/jmm.0.049577-0. View

Li J, Li S, Yang L, Cao P, Lu J . Severe fever with thrombocytopenia syndrome virus: a highly lethal bunyavirus. Crit Rev Microbiol. 2020; 47(1):112-125. DOI: 10.1080/1040841X.2020.1847037. View

Cui L, Ge Y, Qi X, Xu G, Li H, Zhao K . Detection of severe fever with thrombocytopenia syndrome virus by reverse transcription-cross-priming amplification coupled with vertical flow visualization. J Clin Microbiol. 2012; 50(12):3881-5. PMC: 3502986. DOI: 10.1128/JCM.01931-12. View

Chen Y, Zhan Z, Zhou L, Chen M, Cao X, Li Y . Diagnostic value of loop-mediated isothermal amplification assay for hand, foot, and mouth disease. J Clin Lab Anal. 2021; 35(6):e23776. PMC: 8183915. DOI: 10.1002/jcla.23776. View

10.

Takahashi T, Maeda K, Suzuki T, Ishido A, Shigeoka T, Tominaga T . The first identification and retrospective study of Severe Fever with Thrombocytopenia Syndrome in Japan. J Infect Dis. 2013; 209(6):816-27. PMC: 7107388. DOI: 10.1093/infdis/jit603. View

11.

Yoshikawa T . Vaccine Development for Severe Fever with Thrombocytopenia Syndrome. Viruses. 2021; 13(4). PMC: 8067456. DOI: 10.3390/v13040627. View

12.

Cai L, Zhang H, Gao L, Hu S, Xie L, Zhan Z . Identification of the first case of SFTSV infection in the Hunan Province of China and epidemiological surveillance in the locality. Ticks Tick Borne Dis. 2019; 10(2):454-461. DOI: 10.1016/j.ttbdis.2018.11.011. View

13.

Kim K, Yi J, Kim G, Choi S, Jun K, Kim N . Severe fever with thrombocytopenia syndrome, South Korea, 2012. Emerg Infect Dis. 2013; 19(11):1892-4. PMC: 3837670. DOI: 10.3201/eid1911.130792. View

14.

Sun Y, Liang M, Qu J, Jin C, Zhang Q, Li J . Early diagnosis of novel SFTS bunyavirus infection by quantitative real-time RT-PCR assay. J Clin Virol. 2011; 53(1):48-53. DOI: 10.1016/j.jcv.2011.09.031. View

15.

Yu X, Liang M, Zhang S, Liu Y, Li J, Sun Y . Fever with thrombocytopenia associated with a novel bunyavirus in China. N Engl J Med. 2011; 364(16):1523-32. PMC: 3113718. DOI: 10.1056/NEJMoa1010095. View

16.

Odiwuor N, Xiong J, Ogolla F, Hong W, Li X, Khan F . A point-of-care SARS-CoV-2 test based on reverse transcription loop-mediated isothermal amplification without RNA extraction with diagnostic performance same as RT-PCR. Anal Chim Acta. 2022; 1200:339590. PMC: 8844505. DOI: 10.1016/j.aca.2022.339590. View

17.

Liu Q, He B, Huang S, Wei F, Zhu X . Severe fever with thrombocytopenia syndrome, an emerging tick-borne zoonosis. Lancet Infect Dis. 2014; 14(8):763-772. DOI: 10.1016/S1473-3099(14)70718-2. View

18.

Gai Z, Zhang Y, Liang M, Jin C, Zhang S, Zhu C . Clinical progress and risk factors for death in severe fever with thrombocytopenia syndrome patients. J Infect Dis. 2012; 206(7):1095-102. DOI: 10.1093/infdis/jis472. View

19.

Huang M, Liu S, Xu Y, Li A, Wu W, Liang M . CRISPR/Cas12a Technology Combined With RPA for Rapid and Portable SFTSV Detection. Front Microbiol. 2022; 13:754995. PMC: 8822122. DOI: 10.3389/fmicb.2022.754995. View

20.

Sano S, Fukushi S, Yamada S, Harada S, Kinoshita H, Sugimoto S . Development of an RT-LAMP Assay for the Rapid Detection of SFTS Virus. Viruses. 2021; 13(4). PMC: 8073756. DOI: 10.3390/v13040693. View