Peptidome Analysis of Human Intrauterine Adhesion Tissues and the Identification of Antifibrotic Peptide

Overview

Journal J Biomed Res

Specialty General Medicine

Date 2022 Aug 15

PMID 35965437

Authors

Xiangdong Hua

Yan Zhang

Juan Xu

Lu Xu

Yaqian Shi

Dazhen Yang

Xiaoyan Gu

Sumin Wang

Xuemei Jia

Feng Xu

Jie Chen

Xiaoyan Ying

Affiliations

Soon will be listed here.

Abstract

Intrauterine adhesion (IUA) is a common clinical endometrial disease, which can severely damage the fertility and quality of life in women. This study aims to find the differentially expressed endogenous peptides and their possible roles in IUA. Liquid chromatography-mass spectrometry was used to identify the peptidomic profiling of IUA tissues, and the differentially expressed peptides were screened out. Using real-time quantitative PCR, Western blotting, and immunocytochemistry staining, the function of six endogenous peptides was verified . It was found that peptide 6 (T6) (peptide sequence: TFGGAPGFPLGSPLSSVFPR) could inhibit the expression of TGF-β1-induced cell fibrosis in human endometrial stromal cell line and primary human endometrial stromal cell at a concentration of 50 μmol/L. This study provides new targets for further clarifying the formation and prevention of IUA.

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