Impact of Gamma Irradiation on the Properties of Magnesium-Doped Hydroxyapatite in Chitosan Matrix

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2022 Aug 12

PMID 35955308

Authors

Daniela Predoi

Carmen Steluta Ciobanu

Simona Liliana Iconaru

Silviu Adrian Predoi

Mariana Carmen Chifiriuc

Steinar Raaen

Monica Luminita Badea

Krzysztof Rokosz

Affiliations

Soon will be listed here.

Abstract

This is the first report regarding the effect of gamma irradiation on chitosan-coated magnesium-doped hydroxyapatite (x = 0.1; 10 MgHApCh) layers prepared by the spin-coating process. The stability of the resulting 10 MgHApCh gel suspension used to obtain the layers has been shown by ultrasound measurements. The presence of magnesium and the effect of the irradiation process on the studied samples were shown by X-ray photoelectron spectroscopy (XPS). The XPS results obtained for irradiated 10 MgHApCh layers suggested that the magnesium and calcium contained in the surface layer are from tricalcium phosphate (TCP; Ca(PO)) and hydroxyapatite (HAp). The XPS analysis has also highlighted that the amount of TCP in the surface layer increased with the irradiation dose. The energy-dispersive X-ray spectroscopy (EDX) evaluation showed that the calcium decreases with the increase in the irradiation dose. In addition, a decrease in crystallinity and crystallite size was highlighted after irradiation. By atomic force microscopy (AFM) we have obtained images suggesting a good homogeneity of the surface of the non-irradiated and irradiated layers. The AFM results were also sustained by the scanning electron microscopy (SEM) images obtained for the studied samples. The effect of gamma-ray doses on the Fourier transform infrared spectroscopy (ATR-FTIR) spectra of 10 MgHApCh composite layers was also evaluated. The in vitro antifungal assays proved that 10 MgHApCh composite layers presented a strong antifungal effect, correlated with the irradiation dose and incubation time. The study of the stability of the 10 MgHApCh gel allowed us to achieve uniform and homogeneous layers that could be used in different biomedical applications.

Citing Articles

Influence of Electron Beam Irradiation and RPMI Immersion on the Development of Magnesium-Doped Hydroxyapatite/Chitosan Composite Bioactive Layers for Biomedical Applications.

Groza A, Hurjui M, Yehia-Alexe S, Staicu C, Bleotu C, Iconaru S Polymers (Basel). 2025; 17(4).

PMID: 40006195 PMC: 11858993. DOI: 10.3390/polym17040533.

The effect of capping agent on morphology, surface functionalization, and bio-compatibility properties of KTiOPO nanoparticles.

Gharibshahian E, Gharibshahian M, Tafreshi M, Bahraminasab M, Arab S Heliyon. 2024; 10(23):e40513.

PMID: 39654727 PMC: 11626051. DOI: 10.1016/j.heliyon.2024.e40513.

Development of Novel Biocomposites with Antimicrobial-Activity-Based Magnesium-Doped Hydroxyapatite with Amoxicillin.

Cimpeanu C, Predoi D, Ciobanu C, Iconaru S, Rokosz K, Predoi M Antibiotics (Basel). 2024; 13(10).

PMID: 39452229 PMC: 11504330. DOI: 10.3390/antibiotics13100963.

Visible Light-Sensitive Sustainable Quantum Dot Crystals of Co/Mg Doped Natural Hydroxyapatite Possessing Antimicrobial Activity and Biocompatibility.

Maleki-Ghaleh H, Kaminski B, Moradpur-Tari E, Raza S, Khanmohammadi M, Zbonikowski R Small. 2024; 20(52):e2405708.

PMID: 39449217 PMC: 11673459. DOI: 10.1002/smll.202405708.

Chitosan-Clay Mineral Nanocomposites with Antibacterial Activity for Biomedical Application: Advantages and Future Perspectives.

Krajisnik D, Uskokovic-Markovic S, Dakovic A Int J Mol Sci. 2024; 25(19).

PMID: 39408707 PMC: 11476839. DOI: 10.3390/ijms251910377.

References

Rabea E, Badawy M, Stevens C, Smagghe G, Steurbaut W . Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules. 2003; 4(6):1457-65. DOI: 10.1021/bm034130m. View

Sartori M, Giavaresi G, Tschon M, Martini L, Dolcini L, Fiorini M . Long-term in vivo experimental investigations on magnesium doped hydroxyapatite bone substitutes. J Mater Sci Mater Med. 2014; 25(6):1495-504. DOI: 10.1007/s10856-014-5177-5. View

Farkas J . Irradiation as a method for decontaminating food. A review. Int J Food Microbiol. 1998; 44(3):189-204. DOI: 10.1016/s0168-1605(98)00132-9. View

Ciobanu C, Groza A, Iconaru S, Popa C, Chapon P, Chifiriuc M . Antimicrobial Activity Evaluation on Silver Doped Hydroxyapatite/Polydimethylsiloxane Composite Layer. Biomed Res Int. 2015; 2015:926513. PMC: 4609430. DOI: 10.1155/2015/926513. View

Mrazova H, Koller J, Kubisova K, Fujerikova G, Klincova E, Babal P . Comparison of structural changes in skin and amnion tissue grafts for transplantation induced by gamma and electron beam irradiation for sterilization. Cell Tissue Bank. 2015; 17(2):255-60. DOI: 10.1007/s10561-015-9536-3. View

Takeuchi N, Machigashira M, Yamashita D, Shirakata Y, Kasuga T, Noguchi K . Cellular compatibility of a gamma-irradiated modified siloxane-poly(lactic acid)-calcium carbonate hybrid membrane for guided bone regeneration. Dent Mater J. 2011; 30(5):730-8. DOI: 10.4012/dmj.2011-075. View

Zaoutis T, Argon J, Chu J, Berlin J, Walsh T, Feudtner C . The epidemiology and attributable outcomes of candidemia in adults and children hospitalized in the United States: a propensity analysis. Clin Infect Dis. 2005; 41(9):1232-9. DOI: 10.1086/496922. View

Landi E, Logroscino G, Proietti L, Tampieri A, Sandri M, Sprio S . Biomimetic Mg-substituted hydroxyapatite: from synthesis to in vivo behaviour. J Mater Sci Mater Med. 2007; 19(1):239-47. DOI: 10.1007/s10856-006-0032-y. View

Sahariah P, Masson M . Antimicrobial Chitosan and Chitosan Derivatives: A Review of the Structure-Activity Relationship. Biomacromolecules. 2017; 18(11):3846-3868. DOI: 10.1021/acs.biomac.7b01058. View

10.

Taff H, Mitchell K, Edward J, Andes D . Mechanisms of Candida biofilm drug resistance. Future Microbiol. 2013; 8(10):1325-37. PMC: 3859465. DOI: 10.2217/fmb.13.101. View

11.

Ni M, Ratner B . Differentiation of Calcium Carbonate Polymorphs by Surface Analysis Techniques - An XPS and TOF-SIMS study. Surf Interface Anal. 2014; 40(10):1356-1361. PMC: 4096336. DOI: 10.1002/sia.2904. View

12.

Niu X, Feng Q, Wang M, Guo X, Zheng Q . Preparation and characterization of chitosan microspheres for controlled release of synthetic oligopeptide derived from BMP-2. J Microencapsul. 2008; 26(4):297-305. DOI: 10.1080/02652040802319742. View

13.

Halls N . The microbiology of irradiation sterilization. Med Device Technol. 1992; 3(6):37-45. View

14.

Wang M . Developing bioactive composite materials for tissue replacement. Biomaterials. 2003; 24(13):2133-51. DOI: 10.1016/s0142-9612(03)00037-1. View

15.

Rahman N, Khan R, Badshah S . Effect of x-rays and gamma radiations on the bone mechanical properties: literature review. Cell Tissue Bank. 2018; 19(4):457-472. DOI: 10.1007/s10561-018-9736-8. View

16.

Hosny A, Kashef M, Taher H, El-Bazza Z . The use of unirradiated and γ-irradiated zinc oxide nanoparticles as a preservative in cosmetic preparations. Int J Nanomedicine. 2017; 12:6799-6811. PMC: 5602460. DOI: 10.2147/IJN.S143754. View

17.

Guo X, Gough J, Xiao P, Liu J, Shen Z . Fabrication of nanostructured hydroxyapatite and analysis of human osteoblastic cellular response. J Biomed Mater Res A. 2007; 82(4):1022-32. DOI: 10.1002/jbm.a.31200. View

18.

Espigares I, Elvira C, Mano J, Vazquez B, San R, Reis R . New partially degradable and bioactive acrylic bone cements based on starch blends and ceramic fillers. Biomaterials. 2002; 23(8):1883-95. DOI: 10.1016/s0142-9612(01)00315-5. View

19.

Prieto Valdes J, Ortiz Lopez J, Rueda Morales G, Pacheco Malagon G, Prieto Gortcheva V . Fibrous growth of tricalcium phosphate ceramics. J Mater Sci Mater Med. 1997; 8(5):297-301. DOI: 10.1023/a:1018512428683. View

20.

Dreghici D, Butoi B, Predoi D, Iconaru S, Stoican O, Groza A . Chitosan-Hydroxyapatite Composite Layers Generated in Radio Frequency Magnetron Sputtering Discharge: From Plasma to Structural and Morphological Analysis of Layers. Polymers (Basel). 2020; 12(12). PMC: 7767375. DOI: 10.3390/polym12123065. View