A Sulfated Polysaccharide from Red Algae () to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells

Overview

Journal Foods

Specialty Biotechnology

Date 2022 Aug 12

PMID 35954126

Authors

Haiyan Zheng

Yu Pei

Yuan-Lin He

Yi Liu

Minqi Chen

Pengzhi Hong

Chunxia Zhou

Zhong-Ji Qian

Affiliations

Soon will be listed here.

Abstract

Sulfated polysaccharides from red algae have a variety of biological activities, especially antitumor activities. Matrix metalloproteinase-9 (MMP-9) is a proteolytic metalloenzyme that degrades the central part of the extracellular matrix (ECM) and promotes tumor metastasis. In this research, we have investigated the influence and mechanism of GNP (sulfated polysaccharide from ) on tumor metastasis and MMP-9 expression of human fibrosarcoma (HT1080) cells. The results inflected that the concentration of GNP below 100 μg/mL has no toxicity to HT1080 cells, but showed excellent activity in inhibiting cells migration and invasion. In addition, GNP effectively inhibits the mRNA of MMP-9 and reduces its expression and activity by regulating nuclear factor-kappa B (NF-κB), mitogen-activated protein kinases (MAPK) and mTOR/PI3K/Akt signaling pathways. GNP has great potential as MMP-9 inhibitor and could be developed as a functional food or drug to prevent tumor metastasis.

References

Bellan D, Biscaia S, Rossi G, Cristal A, Goncalves J, Oliveira C . Green does not always mean go: A sulfated galactan from Codium isthmocladum green seaweed reduces melanoma metastasis through direct regulation of malignancy features. Carbohydr Polym. 2020; 250:116869. DOI: 10.1016/j.carbpol.2020.116869. View

Lu J, Wang Z, Li S, Xin Q, Yuan M, Li H . Quercetin Inhibits the Migration and Invasion of HCCLM3 Cells by Suppressing the Expression of p-Akt1, Matrix Metalloproteinase (MMP) MMP-2, and MMP-9. Med Sci Monit. 2018; 24:2583-2589. PMC: 5941983. DOI: 10.12659/MSM.906172. View

Batirel S, Mine Yilmaz A, Sahin A, Perakakis N, Ozer N, Mantzoros C . Antitumor and antimetastatic effects of walnut oil in esophageal adenocarcinoma cells. Clin Nutr. 2017; 37(6 Pt A):2166-2171. DOI: 10.1016/j.clnu.2017.10.016. View

Da C, Wu K, Yue C, Bai P, Wang R, Wang G . N-cadherin promotes thyroid tumorigenesis through modulating major signaling pathways. Oncotarget. 2017; 8(5):8131-8142. PMC: 5352388. DOI: 10.18632/oncotarget.14101. View

Boutry J, Tissot S, Ujvari B, Capp J, Giraudeau M, Nedelcu A . The evolution and ecology of benign tumors. Biochim Biophys Acta Rev Cancer. 2021; 1877(1):188643. DOI: 10.1016/j.bbcan.2021.188643. View

Huang H . Matrix Metalloproteinase-9 (MMP-9) as a Cancer Biomarker and MMP-9 Biosensors: Recent Advances. Sensors (Basel). 2018; 18(10). PMC: 6211011. DOI: 10.3390/s18103249. View

Long X, Hu X, Liu S, Pan C, Chen S, Li L . Insights on preparation, structure and activities of polysaccharide. Food Chem X. 2021; 12:100153. PMC: 8591341. DOI: 10.1016/j.fochx.2021.100153. View

Pei Y, Yang S, Xiao Z, Zhou C, Hong P, Qian Z . Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae () and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells. Front Bioeng Biotechnol. 2021; 9:794818. PMC: 8637441. DOI: 10.3389/fbioe.2021.794818. View

Delma C, Thirugnanasambandan S, Srinivasan G, Raviprakash N, Manna S, Natarajan M . Fucoidan from marine brown algae attenuates pancreatic cancer progression by regulating p53 - NFκB crosstalk. Phytochemistry. 2019; 167:112078. DOI: 10.1016/j.phytochem.2019.112078. View

10.

Guo W, Gao X, Zhan R, Zhao Z, Xu K, Tang B . Tricolor imaging of MMPs to investigate the promoting roles of inflammation on invasion and migration of tumor cells. Talanta. 2020; 222:121525. DOI: 10.1016/j.talanta.2020.121525. View

11.

Jung J, Jung K, Kim D, Kim H . Selective inhibition of MMP-9 gene expression by mangiferin in PMA-stimulated human astroglioma cells: involvement of PI3K/Akt and MAPK signaling pathways. Pharmacol Res. 2012; 66(1):95-103. DOI: 10.1016/j.phrs.2012.02.013. View

12.

Guo Y, Pan W, Liu S, Shen Z, Xu Y, Hu L . ERK/MAPK signalling pathway and tumorigenesis. Exp Ther Med. 2020; 19(3):1997-2007. PMC: 7027163. DOI: 10.3892/etm.2020.8454. View

13.

Bauvois B . New facets of matrix metalloproteinases MMP-2 and MMP-9 as cell surface transducers: outside-in signaling and relationship to tumor progression. Biochim Biophys Acta. 2011; 1825(1):29-36. DOI: 10.1016/j.bbcan.2011.10.001. View

14.

Dobbin Z, Landen C . The importance of the PI3K/AKT/MTOR pathway in the progression of ovarian cancer. Int J Mol Sci. 2013; 14(4):8213-27. PMC: 3645739. DOI: 10.3390/ijms14048213. View

15.

Aroui S, Aouey B, Chtourou Y, Meunier A, Fetoui H, Kenani A . Naringin suppresses cell metastasis and the expression of matrix metalloproteinases (MMP-2 and MMP-9) via the inhibition of ERK-P38-JNK signaling pathway in human glioblastoma. Chem Biol Interact. 2016; 244:195-203. DOI: 10.1016/j.cbi.2015.12.011. View

16.

Hsu C, Huang S, Wang J, Chu W, Nien J, Chen W . Interplay of N-Cadherin and matrix metalloproteinase 9 enhances human nasopharyngeal carcinoma cell invasion. BMC Cancer. 2016; 16(1):800. PMC: 5064931. DOI: 10.1186/s12885-016-2846-4. View

17.

Li Y, Zhang M, Dorfman R, Pan Y, Tang D, Xu L . SIRT2 Promotes the Migration and Invasion of Gastric Cancer through RAS/ERK/JNK/MMP-9 Pathway by Increasing PEPCK1-Related Metabolism. Neoplasia. 2018; 20(7):745-756. PMC: 6005814. DOI: 10.1016/j.neo.2018.03.008. View

18.

Iroegbu J, Ijomone O, Femi-Akinlosotu O, Ijomone O . ERK/MAPK signalling in the developing brain: Perturbations and consequences. Neurosci Biobehav Rev. 2021; 131:792-805. DOI: 10.1016/j.neubiorev.2021.10.009. View

19.

Thinh P, Ly B, Usoltseva R, Shevchenko N, Rasin A, Anastyuk S . A novel sulfated fucan from Vietnamese sea cucumber Stichopus variegatus: Isolation, structure and anticancer activity in vitro. Int J Biol Macromol. 2018; 117:1101-1109. DOI: 10.1016/j.ijbiomac.2018.06.017. View

20.

Kramer N, Walzl A, Unger C, Rosner M, Krupitza G, Hengstschlager M . In vitro cell migration and invasion assays. Mutat Res. 2012; 752(1):10-24. DOI: 10.1016/j.mrrev.2012.08.001. View