Characterization of Bacterial Amyloids by Nano-infrared Spectroscopy

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 Aug 11

PMID 35951297

Authors

Vincent Raussens

Jehan Waeytens

Affiliations

Soon will be listed here.

Abstract

Atomic force microscopy has been used for decades to study the topography of proteins during aggregation but with a lack of information on the secondary structure. On the contrary, infrared spectroscopy was able to study structural changes during the aggregation, but this analysis is complicated due to the presence of different species in mixtures and the poor spatial (~μm) resolution of the FTIR microscopy. Recently, Professor Alexandre Dazzi combined those techniques in the so-called AFM-IR. This method allows acquiring IR spectra at the nanometric scale and becomes a new standard method for the characterization of amyloid fibrils and, more generally, for the aggregation of proteins.

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