Phylogenetic and Multiple-Locus Variable Number Tandem Repeat Analysis of Mycobacterium Avium Subsp. Paratuberculosis Isolates from Argentina

Overview

Journal Vet Res Commun

Publisher Springer

Specialty Veterinary Medicine

Date 2022 Aug 10

PMID 35948855

Authors

Brenda Vasini

Pablo Farace

Amadio Ariel

Karina Cirone

Laura Mendez

Claudia Morsella

Pablo Fresia

Gregorio Iraola

Andrea Gioffre

Fernando Paolicchi

Affiliations

Soon will be listed here.

Abstract

Paratuberculosis is a worldwide chronic enteric disease of ruminants, caused by Mycobacterium avium subsp. paratuberculosis (MAP). While MAP has been widely investigated all around the world, little is known about the different strains that circulate in each country. This study describes the genetic diversity of MAP isolates from different bovine and deer herds from Argentina, analyzed by Multiple-Locus Variable number tandem repeat Analysis (MLVA), as well as the phylogenetic relatedness between geographically distant isolates through Whole Genome Sequencing (WGS) and core-genome analysis. A total of 90 MAP isolates were analyzed. The results showed seven different MLVA genotypes, with almost 75% of them belonging to pattern INMV 1, described in all the herds studied. WGS results suggested the presence of a common INMV 1 strain circulating throughout the country. Our results allow confirming the coexistence of different strains in time and space and the mixed infections identified in some animals. These observations suggest the absence of animal monitoring prior to introduction to the herds and the need for a control program in the country. This study represents the first to report WGS of MAP strains in Argentina.

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