Diagnosis and Molecular Characterization of Mycobacterium Avium Subsp. Paratuberculosis from Dairy Cows in Colombia

Overview

Journal Vet Med Int

Publisher Wiley

Specialty Veterinary Medicine

Date 2011 Jul 26

PMID 21785685

Citations 8

Authors

J A Fernandez-Silva

A Abdulmawjood

M Bulte

Affiliations

Soon will be listed here.

Abstract

The objective of this study was the serological, bacteriological and molecular diagnosis, as well as the molecular characterization of Mycobacterium avium subsp. paratuberculosis (Map) in adult cows of five Colombian dairy herds. Serum samples were tested by an indirect absorbed enzyme-linked immunosorbent assay (ELISA-C). All fecal samples were tested by pooled culture. After that, fecal samples of Map positive pools were tested individually by culture and polymerase chain reaction (PCR). In one herd, slurry and tissue samples from one animal were also taken and tested by PCR and culture. Map isolates were analyzed by the Multilocus Short Sequence Repeat (MLSSR) and the Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTR) methods. ELISA produced positive results in 1.8% (6/329) of the animals and 40% (2/5) of the herds. Four fecal, two tissue, and two slurry samples from a herd were Map positive by culture and PCR. MLSSR and MIRU-VNTR revealed two different strain profiles among eight Map isolates recovered. This study reports the first molecular characterization of Map in one dairy herd in Colombia, the limitations for individual diagnosis of subclinical Map infections in cattle, and the usefulness of pooled fecal samples and environmental sampling for Map diagnosis.

Citing Articles

Presence of antibodies against Mycobacterium avium subspecies paratuberculosis in Brazilian high-producing dairy herds.

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Phylogenetic and Multiple-Locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina.

Vasini B, Farace P, Ariel A, Cirone K, Mendez L, Morsella C Vet Res Commun. 2022; 46(4):1121-1129.

PMID: 35948855 DOI: 10.1007/s11259-022-09983-8.

Paratuberculosis in Latin America: a systematic review.

Espeschit I, Schwarz D, Faria A, Souza M, Paolicchi F, Juste R Trop Anim Health Prod. 2017; 49(8):1557-1576.

PMID: 28884331 DOI: 10.1007/s11250-017-1385-6.

Milk yield and lactation stage are associated with positive results to ELISA for Mycobacterium avium subsp. paratuberculosis in dairy cows from Northern Antioquia, Colombia: a preliminary study.

Correa-Valencia N, Ramirez N, Olivera M, Fernandez-Silva J Trop Anim Health Prod. 2016; 48(6):1191-200.

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Molecular typing of Argentinian Mycobacterium avium subsp. paratuberculosis isolates by multiple-locus variable number-tandem repeat analysis.

Gioffre A, Correa Munoz M, Alvarado Pinedo M, Vaca R, Morsella C, Fiorentino M Braz J Microbiol. 2015; 46(2):557-64.

PMID: 26273274 PMC: 4507551. DOI: 10.1590/S1517-838246220140283.

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