Dental Age Estimation Using Multiphoton Microscopy: A Potential Tool for Forensic Science

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2022 Aug 8

PMID 35937389

Authors

Juan M Bueno

Rosa M Martinez-Ojeda

Ana C Fernandez-Escudero

Francisco J Avila

Manuel Lopez-Nicolas

Maria D Perez-Carceles

Affiliations

Soon will be listed here.

Abstract

Normal aging affects the different structures of teeth, in particular, the dentine. These changes are useful in forensic disciplines as a tool for age estimation. Although multiphoton (MP) microscopy has been used to explore dental pieces, a relationship between age and MP response of the human dentine has not been proposed yet. The relationship between MP signals and natural dentine aging is investigated herein. An index of age (INAG) combining two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) images has been used to quantify these changes. The results show that the INAG significantly decreases with age. Moreover, peritubular dentine size and collagen internal properties are also modified with age. This information confirms the usefulness of this technique in forensic age estimation after disasters (natural or manmade) with a lack of comprehensive fingerprint database. Courts and other government authorities might also benefit from this tool when the official age of individuals under special circumstances is required for legal or medical reasons.

Citing Articles

Radiological Evaluation of the Accuracy of Demirjian, Nolla, and Willems Methods for Dental Age Estimation in 3-17-Year-Old Iranian Children.

Pliska B, Nahvi A, Pakdaman N, Dadgar S, Aryana M, Sobouti F Biomed Res Int. 2024; 2024:8783660.

PMID: 38988904 PMC: 11236468. DOI: 10.1155/2024/8783660.

Analysis of age-related changes in the left ventricular myocardium with multiphoton microscopy.

Bueno J, Martinez-Ojeda R, Perez-Zabalza M, Garcia-Mendivil L, Asensio M, Ordovas L Biomed Opt Express. 2024; 15(5):3251-3264.

PMID: 38855691 PMC: 11161339. DOI: 10.1364/BOE.509227.

References

Stanciu S, Avila F, Hristu R, Bueno J . A Study on Image Quality in Polarization-Resolved Second Harmonic Generation Microscopy. Sci Rep. 2017; 7(1):15476. PMC: 5684207. DOI: 10.1038/s41598-017-15257-0. View

Kinney J, Pople J, Marshall G, Marshall S . Collagen orientation and crystallite size in human dentin: a small angle X-ray scattering study. Calcif Tissue Int. 2001; 69(1):31-7. DOI: 10.1007/s00223-001-0006-5. View

Avila F, Bueno J . Analysis and quantification of collagen organization with the structure tensor in second harmonic microscopy images of ocular tissues. Appl Opt. 2016; 54(33):9848-54. DOI: 10.1364/AO.54.009848. View

Koutsi V, NOONAN R, HORNER J, Simpson M, Matthews W, Pashley D . The effect of dentin depth on the permeability and ultrastructure of primary molars. Pediatr Dent. 1994; 16(1):29-35. View

Maber M, Liversidge H, Hector M . Accuracy of age estimation of radiographic methods using developing teeth. Forensic Sci Int. 2006; 159 Suppl 1:S68-73. DOI: 10.1016/j.forsciint.2006.02.019. View

Chen S, Hsu C, Sun C . Epi-third and second harmonic generation microscopic imaging of abnormal enamel. Opt Express. 2008; 16(15):11670-9. View

Eldarrat A, Wood D, Kale G, High A . Age-related changes in ac-impedance spectroscopy studies of normal human dentine. J Mater Sci Mater Med. 2007; 18(6):1203-10. DOI: 10.1007/s10856-007-0136-z. View

Hall A, Girkin J . A review of potential new diagnostic modalities for caries lesions. J Dent Res. 2004; 83 Spec No C:C89-94. DOI: 10.1177/154405910408301s18. View

Tan H, Sun Y, Lo W, Teng S, Wu R, Jee S . Multiphoton fluorescence and second harmonic generation microscopy for imaging infectious keratitis. J Biomed Opt. 2007; 12(2):024013. DOI: 10.1117/1.2717133. View

10.

Ait-Belkacem D, Guilbert M, Roche M, Duboisset J, Ferrand P, Sockalingum G . Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation. J Biomed Opt. 2012; 17(8):080506-1. DOI: 10.1117/1.JBO.17.8.080506. View

11.

Ager 3rd J, Nalla R, Balooch G, Kim G, Pugach M, Habelitz S . On the increasing fragility of human teeth with age: a deep-UV resonance Raman study. J Bone Miner Res. 2006; 21(12):1879-87. DOI: 10.1359/jbmr.060816. View

12.

Stoller P, Reiser K, Celliers P, Rubenchik A . Polarization-modulated second harmonic generation in collagen. Biophys J. 2002; 82(6):3330-42. PMC: 1302120. DOI: 10.1016/S0006-3495(02)75673-7. View

13.

Williams R, Zipfel W, Webb W . Interpreting second-harmonic generation images of collagen I fibrils. Biophys J. 2004; 88(2):1377-86. PMC: 1305140. DOI: 10.1529/biophysj.104.047308. View

14.

Lin P, Lyu H, Stephen Hsu C, Chang C, Kao F . Imaging carious dental tissues with multiphoton fluorescence lifetime imaging microscopy. Biomed Opt Express. 2011; 2(1):149-58. PMC: 3028490. DOI: 10.1364/BOE.2.000149. View

15.

Ruschel H, Chevitarese O . Density and diameter of dentinal tubules of first and second primary human molars--comparative scanning electron microscopy study. J Clin Pediatr Dent. 2002; 26(3):297-304. DOI: 10.17796/jcpd.26.3.t5344598326r5687. View

16.

Van Gulick L, Saby C, Morjani H, Beljebbar A . Age-related changes in molecular organization of type I collagen in tendon as probed by polarized SHG and Raman microspectroscopy. Sci Rep. 2019; 9(1):7280. PMC: 6513820. DOI: 10.1038/s41598-019-43636-2. View

17.

Baumgartner A, Dichtl S, Hitzenberger C, Sattmann H, Robl B, Moritz A . Polarization-sensitive optical coherence tomography of dental structures. Caries Res. 1999; 34(1):59-69. DOI: 10.1159/000016571. View

18.

Fernandez-Escudero A, Legaz I, Prieto-Bonete G, Lopez-Nicolas M, Maurandi-Lopez A, Perez-Carceles M . Aging and trace elements in human coronal tooth dentine. Sci Rep. 2020; 10(1):9964. PMC: 7305194. DOI: 10.1038/s41598-020-66472-1. View

19.

Pretty I, Sweet D . A look at forensic dentistry--Part 1: The role of teeth in the determination of human identity. Br Dent J. 2001; 190(7):359-66. DOI: 10.1038/sj.bdj.4800972. View

20.

Alqahtani S, Hector M, Liversidge H . Brief communication: The London atlas of human tooth development and eruption. Am J Phys Anthropol. 2010; 142(3):481-90. DOI: 10.1002/ajpa.21258. View