Imaging Carious Dental Tissues with Multiphoton Fluorescence Lifetime Imaging Microscopy

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2011 Feb 18

PMID 21326645

Citations 5

Authors

Po-Yen Lin

Hong-Chou Lyu

Chin-Ying Stephen Hsu

Chia-Seng Chang

Fu-Jen Kao

Affiliations

Soon will be listed here.

Abstract

In this study, multiphoton excitation was utilized to image normal and carious dental tissues noninvasively. Unique structures in dental tissues were identified using the available multimodality (second harmonic, autofluorescence, and fluorescence lifetime analysis) without labeling. The collagen in dentin exhibits a strong second harmonic response. Both dentin and enamel emit strong autofluorescence that reveals in detail morphological features (such as dentinal tubules and enamel rods) and, despite their very similar spectral profiles, can be differentiated by lifetime analysis. Specifically, the carious dental tissue exhibits a greatly reduced autofluorescence lifetime, which result is consistent with the degree of demineralization, determined by micro-computed tomography. Our findings suggest that two-photon excited fluorescence lifetime imaging may be a promising tool for diagnosing and monitoring dental caries.

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