Modelling Reoxygenation Effects in Non-small Cell Lung Cancer Cell Lines and Showing Epithelial-mesenchymal Transition

Overview

Journal J Cancer Res Clin Oncol

Specialty Oncology

Date 2022 Aug 6

PMID 35932303

Authors

Joanna Kapeleris

Juliana Muller Bark

Shanon Ranjit

Derek Richard

Ian Vela

Kenneth OByrne

Chamindie Punyadeera

Affiliations

Soon will be listed here.

Abstract

Purpose: Circulating tumour cells (CTCs) are a rare cell subpopulation regulated by the tumour microenvironment. In hypoxic conditions, CTCs are able to invade the lymphatic and circulatory systems leading to metastasis at distant sites.

Methods: To mimic in vivo oxygen variations and effects on CTCs, we have cultured five non-small cell lung cancer (NSCLC) cell lines under normoxic and hypoxic conditions, followed by a pulse of reoxygenation for 4 h.

Results: Proliferation, spheroid-formation and colony formation ability under varying O levels were investigated. Proliferation rate was not altered when cells were cultured in 2D models under hypoxic conditions. However, we observed that hypoxia enhanced in vitro formation of tumour-spheres and accelerated clonogenicity of NSCLC cell lines. In addition, cells exposed to hypoxia and reoxygenation conditions showed altered expression of epithelial-mesenchymal transition (EMT) related genes in NSCLC cell lines both at mRNA (AKT1, CAMK2NH1, DESI1, VIM, MAP1B, EGFR, ZEB1, HIF1α) and protein levels (Vimentin, Pan-cytokeratin).

Conclusion: Our data suggest that when investigating CTCs as a prognostic biomarker in NSCLC, it is also essential to take into consideration EMT status to obtain a comprehensive overview of CTCs in circulation.

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