Pyrroloquinoline Quinone (PQQ) Protects Mitochondrial Function of HEI-OC1 Cells Under Premature Senescence

Overview

Journal NPJ Aging

Publisher Springer Nature

Specialty Geriatrics

Date 2022 Aug 4

PMID 35927260

Authors

Ying Gao

Teru Kamogashira

Chisato Fujimoto

Shinichi Iwasaki

Tatsuya Yamasoba

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate the effects of pyrroloquinoline quinone (PQQ), an oxidoreductase cofactor, on the HO-induced premature senescence model in HEI-OC1 auditory cells and to elucidate its mechanism of action in vitro. Cells were treated with PQQ for 1 day before HO (100 μM) exposure. Mitochondrial respiratory capacity was damaged in this premature senescence model but was restored in cells pretreated with PQQ (0.1 nM or 1.0 nM). A decrease in mitochondrial potential, the promotion of mitochondrial fusion and the accelerated movement of mitochondria were all observed in PQQ-pretreated cells. The protein expression of sirtuin 1 (SIRT1) and peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) were significantly decreased under HO exposure while they were increased with PQQ pretreatment, and PGC-1α acetylation was significantly decreased. In conclusion, PQQ has a protective effect on the premature senescence model of HEI-OC1 auditory cells and is associated with the SIRT1/PGC-1α signaling pathway, mitochondrial structure, and mitochondrial respiratory capacity.

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