Pyrroloquinoline Quinone Preserves Mitochondrial Function and Prevents Oxidative Injury in Adult Rat Cardiac Myocytes

Overview

Journal Biochem Biophys Res Commun

Publisher Elsevier

Specialty Biochemistry

Date 2007 Sep 21

PMID 17880922

Citations 37

Authors

Rong Tao

Joel S Karliner

Ursula Simonis

Jie Zheng

Jianqing Zhang

Norman Honbo

Conrad C Alano

Affiliations

Soon will be listed here.

Abstract

We investigated the ability of pyrroloquinoline quinone (PQQ) to confer resistance to acute oxidative stress in freshly isolated adult male rat cardiomyocytes. Fluorescence microscopy was used to detect generation of reactive oxygen species (ROS) and mitochondrial membrane potential (Deltapsi(m)) depolarization induced by hydrogen peroxide. H(2)O(2) caused substantial cell death, which was significantly reduced by preincubation with PQQ. H(2)O(2) also caused an increase in cellular ROS levels as detected by the fluorescent indicators CM-H2XRos and dihydroethidium. ROS levels were significantly reduced by a superoxide dismutase mimetic Mn (III) tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP) or by PQQ treatment. Cyclosporine-A, which inhibits mitochondrial permeability transition, prevented H(2)O(2)-induced Deltapsi(m) depolarization, as did PQQ and MnTBAP. Our results provide direct evidence that PQQ reduces oxidative stress, mitochondrial dysfunction, and cell death in isolated adult rat cardiomyocytes. These findings provide new insight into the mechanisms of PQQ action in the heart.

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References

Zorov D, Filburn C, Klotz L, Zweier J, Sollott S . Reactive oxygen species (ROS)-induced ROS release: a new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes. J Exp Med. 2000; 192(7):1001-14. PMC: 2193314. DOI: 10.1084/jem.192.7.1001. View

Zhu B, Simonis U, Cecchini G, Zhou H, Li L, Teerlink J . Comparison of pyrroloquinoline quinone and/or metoprolol on myocardial infarct size and mitochondrial damage in a rat model of ischemia/reperfusion injury. J Cardiovasc Pharmacol Ther. 2006; 11(2):119-28. DOI: 10.1177/1074248406288757. View

Droge W . Free radicals in the physiological control of cell function. Physiol Rev. 2002; 82(1):47-95. DOI: 10.1152/physrev.00018.2001. View

Tao R, Zhang J, Vessey D, Honbo N, Karliner J . Deletion of the sphingosine kinase-1 gene influences cell fate during hypoxia and glucose deprivation in adult mouse cardiomyocytes. Cardiovasc Res. 2007; 74(1):56-63. DOI: 10.1016/j.cardiores.2007.01.015. View

Zhang Y, Rosenberg P . The essential nutrient pyrroloquinoline quinone may act as a neuroprotectant by suppressing peroxynitrite formation. Eur J Neurosci. 2002; 16(6):1015-24. DOI: 10.1046/j.1460-9568.2002.02169.x. View

Lemasters J, Qian T, He L, Kim J, Elmore S, Cascio W . Role of mitochondrial inner membrane permeabilization in necrotic cell death, apoptosis, and autophagy. Antioxid Redox Signal. 2002; 4(5):769-81. DOI: 10.1089/152308602760598918. View

He K, Nukada H, Urakami T, Murphy M . Antioxidant and pro-oxidant properties of pyrroloquinoline quinone (PQQ): implications for its function in biological systems. Biochem Pharmacol. 2002; 65(1):67-74. DOI: 10.1016/s0006-2952(02)01453-3. View

Levraut J, Iwase H, Shao Z, Vanden Hoek T, Schumacker P . Cell death during ischemia: relationship to mitochondrial depolarization and ROS generation. Am J Physiol Heart Circ Physiol. 2002; 284(2):H549-58. DOI: 10.1152/ajpheart.00708.2002. View

Finkel T . Oxidant signals and oxidative stress. Curr Opin Cell Biol. 2003; 15(2):247-54. DOI: 10.1016/s0955-0674(03)00002-4. View

10.

Kwon S, Pimentel D, Remondino A, Sawyer D, Colucci W . H(2)O(2) regulates cardiac myocyte phenotype via concentration-dependent activation of distinct kinase pathways. J Mol Cell Cardiol. 2003; 35(6):615-21. DOI: 10.1016/s0022-2828(03)00084-1. View

11.

Di Lisa F, Canton M, Menabo R, Dodoni G, Bernardi P . Mitochondria and reperfusion injury. The role of permeability transition. Basic Res Cardiol. 2003; 98(4):235-41. DOI: 10.1007/s00395-003-0415-x. View

12.

Marczin N, El-Habashi N, Hoare G, Bundy R, Yacoub M . Antioxidants in myocardial ischemia-reperfusion injury: therapeutic potential and basic mechanisms. Arch Biochem Biophys. 2003; 420(2):222-36. DOI: 10.1016/j.abb.2003.08.037. View

13.

Hausenloy D, Duchen M, Yellon D . Inhibiting mitochondrial permeability transition pore opening at reperfusion protects against ischaemia-reperfusion injury. Cardiovasc Res. 2003; 60(3):617-25. DOI: 10.1016/j.cardiores.2003.09.025. View

14.

Gum E, Swanson R, Alano C, Liu J, Hong S, Weinstein P . Human serum albumin and its N-terminal tetrapeptide (DAHK) block oxidant-induced neuronal death. Stroke. 2004; 35(2):590-5. DOI: 10.1161/01.STR.0000110790.05859.DA. View

15.

Becker L . New concepts in reactive oxygen species and cardiovascular reperfusion physiology. Cardiovasc Res. 2004; 61(3):461-70. DOI: 10.1016/j.cardiores.2003.10.025. View

16.

Alano C, Ying W, Swanson R . Poly(ADP-ribose) polymerase-1-mediated cell death in astrocytes requires NAD+ depletion and mitochondrial permeability transition. J Biol Chem. 2004; 279(18):18895-902. DOI: 10.1074/jbc.M313329200. View

17.

Shanker G, Aschner J, Syversen T, Aschner M . Free radical formation in cerebral cortical astrocytes in culture induced by methylmercury. Brain Res Mol Brain Res. 2004; 128(1):48-57. DOI: 10.1016/j.molbrainres.2004.05.022. View

18.

Jolly S, Kane W, Bailie M, Abrams G, Lucchesi B . Canine myocardial reperfusion injury. Its reduction by the combined administration of superoxide dismutase and catalase. Circ Res. 1984; 54(3):277-85. DOI: 10.1161/01.res.54.3.277. View

19.

Smidt C, Steinberg F, Rucker R . Physiologic importance of pyrroloquinoline quinone. Proc Soc Exp Biol Med. 1991; 197(1):19-26. DOI: 10.3181/00379727-197-43218. View

20.

Jensen F, Gardner G, Williams A, Gallop P, Aizenman E, Rosenberg P . The putative essential nutrient pyrroloquinoline quinone is neuroprotective in a rodent model of hypoxic/ischemic brain injury. Neuroscience. 1994; 62(2):399-406. DOI: 10.1016/0306-4522(94)90375-1. View