Yeast ORC Sumoylation Status Fine-tunes Origin Licensing

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2022 Aug 4

PMID 35926881

Authors

Gemma Regan-Mochrie

Timothy Hoggard

Nikhil Bhagwat

Gerard Lynch

Neil Hunter

Dirk Remus

Catherine A Fox

Xiaolan Zhao

Affiliations

Soon will be listed here.

Abstract

Sumoylation is emerging as a posttranslation modification important for regulating chromosome duplication and stability. The origin recognition complex (ORC) that directs DNA replication initiation by loading the MCM replicative helicase onto origins is sumoylated in both yeast and human cells. However, the biological consequences of ORC sumoylation are unclear. Here we report the effects of hypersumoylation and hyposumoylation of yeast ORC on ORC activity and origin function using multiple approaches. ORC hypersumoylation preferentially reduced the function of a subset of early origins, while Orc2 hyposumoylation had an opposing effect. Mechanistically, ORC hypersumoylation reduced MCM loading in vitro and diminished MCM chromatin association in vivo. Either hypersumoylation or hyposumoylation of ORC resulted in genome instability and the dependence of yeast on other genome maintenance factors, providing evidence that appropriate ORC sumoylation levels are important for cell fitness. Thus, yeast ORC sumoylation status must be properly controlled to achieve optimal origin function across the genome and genome stability.

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