Dielectrophoretic Traps for Efficient Bead and Cell Trapping and Formation of Aggregates of Controlled Size and Composition

Overview

Journal Front Bioeng Biotechnol

Date 2022 Aug 1

PMID 35910025

Authors

Clementine Lipp

Laure Koebel

Arnaud Bertsch

Michael Gauthier

Aude Bolopion

Philippe Renaud

Affiliations

Soon will be listed here.

Abstract

We present a microfluidic dielectrophoretic-actuated system designed to trap chosen single-cell and form controlled cell aggregates. A novel method is proposed to characterize the efficiency of the dielectrophoretic trapping, considering the flow speed but also the heat generated by the traps as limiting criteria in cell-safe manipulation. Two original designs with different manufacturing processes are experimentally compared. The most efficient design is selected and the cell membrane integrity is monitored by fluorescence imaging to guarantee a safe-cell trapping. Design rules are suggested to adapt the traps to multiple-cells trapping and are experimentally validated as we formed aggregates of controlled size and composition with two different types of cells. We provide hereby a simple manufactured tool allowing the controlled manipulation of particles for the composition of multicellular assemblies.

Citing Articles

On-chip dielectrophoretic single-cell manipulation.

Tian Z, Wang X, Chen J Microsyst Nanoeng. 2024; 10(1):117.

PMID: 39187499 PMC: 11347631. DOI: 10.1038/s41378-024-00750-0.

Microfluidic device combining hydrodynamic and dielectrophoretic trapping for the controlled contact between single micro-sized objects and application to adhesion assays.

Lipp C, Koebel L, Loyon R, Bolopion A, Spehner L, Gauthier M Lab Chip. 2023; 23(16):3593-3602.

PMID: 37458004 PMC: 10408363. DOI: 10.1039/d3lc00400g.

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