Comparative Analysis of Chloroplast Genome in Spp. and Related Members of ' Complex'

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Jul 27

PMID 35887005

Authors

Sicheng Li

Weixing Duan

Jihan Zhao

Yanfen Jing

Mengfan Feng

Bowen Kuang

Ni Wei

Baoshan Chen

Xiping Yang

Affiliations

Soon will be listed here.

Abstract

High ploids of the sugarcane nuclear genome limit its genomic studies, whereas its chloroplast genome is small and conserved, which is suitable for phylogenetic studies and molecular marker development. Here, we applied whole genome sequencing technology to sequence and assemble chloroplast genomes of eight species of the ' Complex', and elucidated their sequence variations. In total, 19 accessions were sequenced, and 23 chloroplast genomes were assembled, including 6 species of (among them, , , and firstly reported in this study) and 2 sugarcane relative species, and . The plastid phylogenetic signal demonstrated that and shared a common ancestor, and that the cytoplasmic origins of and were much more ancient than the / linage. Overall, 14 markers were developed, including 9 InDel markers for distinguishing from its relative species, 4 dCAPS markers for distinguishing from and 1 dCAPS marker for distinguishing and from other species. The results obtained from our studies will contribute to the understanding of the classification and plastome evolution of Saccharinae, and the molecular markers developed have demonstrated their highly discriminatory power in and relative species.

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