Aptamer-Based Biosensors for the Colorimetric Detection of Blood Biomarkers: Paving the Way to Clinical Laboratory Testing

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2022 Jul 27

PMID 35884911

Authors

Anna Davydova

Mariya Vorobyeva

Affiliations

Soon will be listed here.

Abstract

Clinical diagnostics for human diseases rely largely on enzyme immunoassays for the detection of blood biomarkers. Nevertheless, antibody-based test systems have a number of shortcomings that have stimulated a search for alternative diagnostic assays. Oligonucleotide aptamers are now considered as promising molecular recognizing elements for biosensors (aptasensors) due to their high affinity and specificity of target binding. At the moment, a huge variety of aptasensors have been engineered for the detection of various analytes, especially disease biomarkers. However, despite their great potential and excellent characteristics in model systems, only a few of these aptamer-based assays have been translated into practice as diagnostic kits. Here, we will review the current progress in the engineering of aptamer-based colorimetric assays as the most suitable format for clinical lab diagnostics. In particular, we will focus on aptasensors for the detection of blood biomarkers of cardiovascular, malignant, and neurodegenerative diseases along with common inflammation biomarkers. We will also analyze the main obstacles that have to be overcome before aptamer test systems can become tantamount to ELISA for clinical diagnosis purposes.

Citing Articles

Lattice matching enables construction of CaS@NaYF heterostructure with synergistically enhanced water resistance and luminescence for antibiotic detection.

Wang Y, Chen H, Zhao T, Wang J, Wu Y, Liu J Mikrochim Acta. 2024; 191(8):485.

PMID: 39060720 DOI: 10.1007/s00604-024-06568-x.

Computational Frontiers in Aptamer-Based Nanomedicine for Precision Therapeutics: A Comprehensive Review.

Kumar S, Mohan A, Sharma N, Kumar A, Girdhar M, Malik T ACS Omega. 2024; 9(25):26838-26862.

PMID: 38947800 PMC: 11209897. DOI: 10.1021/acsomega.4c02466.

Biosensing Platforms for Cardiac Biomarker Detection.

Gerdan Z, Saylan Y, Denizli A ACS Omega. 2024; 9(9):9946-9960.

PMID: 38463295 PMC: 10918812. DOI: 10.1021/acsomega.3c06571.

A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF) based on anti-VEGF-iron oxide nanoparticle conjugation.

Ceylan H, Kirbay F, Yazgan I, Elibol M Mikrochim Acta. 2024; 191(3):133.

PMID: 38353782 PMC: 10867064. DOI: 10.1007/s00604-024-06228-0.

Aptasensors Based on Non-Enzymatic Peroxidase Mimics: Current Progress and Challenges.

Davydova A, Vorobyeva M Biosensors (Basel). 2024; 14(1).

PMID: 38275302 PMC: 10813519. DOI: 10.3390/bios14010001.

References

Lin M, Li W, Wang Y, Yang X, Wang K, Wang Q . Discrimination of hemoglobins with subtle differences using an aptamer based sensing array. Chem Commun (Camb). 2015; 51(39):8304-6. DOI: 10.1039/c5cc00929d. View

Wang Y, Liu J, Adkins G, Shen W, Trinh M, Duan L . Enhancement of the Intrinsic Peroxidase-Like Activity of Graphitic Carbon Nitride Nanosheets by ssDNAs and Its Application for Detection of Exosomes. Anal Chem. 2017; 89(22):12327-12333. PMC: 5705088. DOI: 10.1021/acs.analchem.7b03335. View

Duanghathaipornsuk S, Reaver N, Cameron B, Kim D . Adsorption Kinetics of Glycated Hemoglobin on Aptamer Microarrays with Antifouling Surface Modification. Langmuir. 2021; 37(15):4647-4657. DOI: 10.1021/acs.langmuir.1c00446. View

Antonio M, Ferreira R, Vitorino R, Daniel-da-Silva A . A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles. Talanta. 2020; 214:120868. DOI: 10.1016/j.talanta.2020.120868. View

Celikbas E, Balaban S, Evran S, Coskunol H, Timur S . A Bottom-Up Approach for Developing Aptasensors for Abused Drugs: Biosensors in Forensics. Biosensors (Basel). 2019; 9(4). PMC: 6955935. DOI: 10.3390/bios9040118. View

Sharma T, Bruno J, Dhiman A . ABCs of DNA aptamer and related assay development. Biotechnol Adv. 2017; 35(2):275-301. DOI: 10.1016/j.biotechadv.2017.01.003. View

Morgan 3rd C, Rasmusson A, Wang S, Hoyt G, Hauger R, Hazlett G . Neuropeptide-Y, cortisol, and subjective distress in humans exposed to acute stress: replication and extension of previous report. Biol Psychiatry. 2002; 52(2):136-42. DOI: 10.1016/s0006-3223(02)01319-7. View

Tan F, Wang Z, Yang Y, Xie X, Hua X, Yang X . Facile preparation of peroxidase-like core-shell nanorods and application as platform for colorimetric determination of glucose, insulin and glucose/insulin ratio. Talanta. 2019; 204:285-293. DOI: 10.1016/j.talanta.2019.06.005. View

Tarkowski E, Issa R, Sjogren M, Wallin A, Blennow K, Tarkowski A . Increased intrathecal levels of the angiogenic factors VEGF and TGF-beta in Alzheimer's disease and vascular dementia. Neurobiol Aging. 2002; 23(2):237-43. DOI: 10.1016/s0197-4580(01)00285-8. View

10.

Storkebaum E, Lambrechts D, Carmeliet P . VEGF: once regarded as a specific angiogenic factor, now implicated in neuroprotection. Bioessays. 2004; 26(9):943-54. DOI: 10.1002/bies.20092. View

11.

Nonaka Y, Sode K, Ikebukuro K . Screening and improvement of an anti-VEGF DNA aptamer. Molecules. 2010; 15(1):215-25. PMC: 6256979. DOI: 10.3390/molecules15010215. View

12.

Lis-Swiety A, Widuchowska M, Brzezinska-Wcislo L, Kucharz E . High acute phase protein levels correlate with pulmonary and skin involvement in patients with diffuse systemic sclerosis. J Int Med Res. 2018; 46(4):1634-1639. PMC: 6091829. DOI: 10.1177/0300060518760955. View

13.

Dalirirad S, Steckl A . Lateral flow assay using aptamer-based sensing for on-site detection of dopamine in urine. Anal Biochem. 2020; 596:113637. DOI: 10.1016/j.ab.2020.113637. View

14.

Almusharraf A, Eissa S, Zourob M . Truncated aptamers for total and glycated hemoglobin, and their integration into a graphene oxide-based fluorometric method for high-throughput screening for diabetes. Mikrochim Acta. 2018; 185(5):256. DOI: 10.1007/s00604-018-2789-3. View

15.

Bao X, Huo G, Li L, Cao X, Liu Y, Lakshmipriya T . Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis. J Anal Methods Chem. 2019; 2019:5676159. PMC: 6881590. DOI: 10.1155/2019/5676159. View

16.

Walsh R, DeRosa M . Retention of function in the DNA homolog of the RNA dopamine aptamer. Biochem Biophys Res Commun. 2009; 388(4):732-5. DOI: 10.1016/j.bbrc.2009.08.084. View

17.

Sezaki S, Hirohata S, Iwabu A, Nakamura K, Toeda K, Miyoshi T . Thrombospondin-1 is induced in rat myocardial infarction and its induction is accelerated by ischemia/reperfusion. Exp Biol Med (Maywood). 2005; 230(9):621-30. DOI: 10.1177/153537020523000904. View

18.

Scheller J, Chalaris A, Schmidt-Arras D, Rose-John S . The pro- and anti-inflammatory properties of the cytokine interleukin-6. Biochim Biophys Acta. 2011; 1813(5):878-88. DOI: 10.1016/j.bbamcr.2011.01.034. View

19.

Liu L, Wang F, Ge Y, Lo P . Recent Developments in Aptasensors for Diagnostic Applications. ACS Appl Mater Interfaces. 2020; 13(8):9329-9358. DOI: 10.1021/acsami.0c14788. View

20.

Martin J, Chavez J, Chushak Y, Chapleau R, Hagen J, Kelley-Loughnane N . Tunable stringency aptamer selection and gold nanoparticle assay for detection of cortisol. Anal Bioanal Chem. 2014; 406(19):4637-47. DOI: 10.1007/s00216-014-7883-8. View