Identification of Body Size Determination Related Candidate Genes in Domestic Pig Using Genome-Wide Selection Signal Analysis

Overview

Journal Animals (Basel)

Date 2022 Jul 27

PMID 35883386

Authors

Bing Pan

Haoyuan Long

Ying Yuan

Haoyuan Zhang

Yangyang Peng

Dongke Zhou

Chengli Liu

Baiju Xiang

Yongfu Huang

Yongju Zhao

Zhongquan Zhao

Guangxin E

Affiliations

Soon will be listed here.

Abstract

This study aimed to identify the genes related to the body size of pigs by conducting genome-wide selection analysis (GWSA). We performed a GWSA scan on 50 pigs belonging to four small-bodied pig populations (Diannan small-eared pig, Bama Xiang pig, Wuzhishan pig, and Jeju black pig from South Korea) and 124 large-bodied pigs. We used the genetic parameters of the pairwise fixation index (F) and π ratio (case/control) to screen candidate genome regions and genes related to body size. The results revealed 47,339,509 high-quality SNPs obtained from 174 individuals, while 280 interacting candidate regions were obtained from the top 1% signal windows of both parameters, along with 187 genes (e.g., , , , , and ). The results of the candidate gene (CG) annotation showed that a series of CGs (e.g., , , , , , and ) was enriched into the gene ontology terms. Moreover, molecular pathways, such as the PI3K-Akt, HIF-1, and AMPK signaling pathways, were verified to be related to body development. Overall, we identified a series of key genes that may be closely related to the body size of pigs, further elucidating the heredity basis of body shape determination in pigs and providing a theoretical reference for molecular breeding.

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