Construction of Antibacterial Nano-silver Embedded Bioactive Hydrogel to Repair Infectious Skin Defects

Overview

Journal Biomater Res

Specialty Biomedical Engineering

Date 2022 Jul 25

PMID 35879746

Authors

Qi Dong

Dan Zu

Lingqiang Kong

Sunfang Chen

Jun Yao

Jiawei Lin

Lei Lu

Bing Wu

Bin Fang

Affiliations

Soon will be listed here.

Abstract

Background: Hydrogels loaded with antimicrobial agents have been widely used for treating infected wound defects. However, hydrogels derived from a porcine dermal extracellular matrix (PADM), containing silver nanoparticles (AgNPs), have not yet been studied. Therefore, we investigated the therapeutic effect of an AgNP-impregnated PADM (AgNP-PADM) hydrogel on the treatment of infected wounds.

Methods: An AgNP-PADM hydrogel was synthesized by embedding AgNPs into a PADM hydrogel. We examined the porosity, moisture retention, degradation, antibacterial properties, cytotoxicity, antioxidant properties, and ability of the PADM and AgNP-PADM hydrogels to treat infected wounds in animals.

Results: The PADM and AgNP-PADM hydrogels were pH sensitive, which made them flow dynamically and solidify under acidic and neutral conditions, respectively. The hydrogels also exhibited porous network structures, satisfactory moisture retention, and slow degradation. Additionally, the AgNP-PADM hydrogel showed a slow and sustained release of AgNPs for at least 7 days without the particle size changing. Thus, the AgNPs exhibited adequate antibacterial ability, negligible toxicity, and antioxidant properties in vitro. Moreover, the AgNP-PADM hydrogel promoted angiogenesis and healed infected skin defects in vivo.

Conclusions: The AgNP-PADM hydrogel is a promising bioderived antibacterial material for clinical application to infected wound dressings.

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References

Gong D, Yu F, Zhou M, Dong W, Yan D, Zhang S . and Properties of an Injectable Hydrogel Derived From Acellular Ear Cartilage Extracellular Matrix. Front Bioeng Biotechnol. 2021; 9:740635. PMC: 8474061. DOI: 10.3389/fbioe.2021.740635. View

Haidari H, Kopecki Z, Bright R, Cowin A, Garg S, Goswami N . Ultrasmall AgNP-Impregnated Biocompatible Hydrogel with Highly Effective Biofilm Elimination Properties. ACS Appl Mater Interfaces. 2020; 12(37):41011-41025. DOI: 10.1021/acsami.0c09414. View

Wakabayashi N, Sakai A, Takada H, Hoshi T, Sano H, Ichinose S . Noncontact Phased-Array Ultrasound Facilitates Acute Wound Healing in Mice. Plast Reconstr Surg. 2020; 145(2):348e-359e. PMC: 7004446. DOI: 10.1097/PRS.0000000000006481. View

Slepicka P, Slepickova Kasalkova N, Siegel J, Kolska Z, Svorcik V . Methods of Gold and Silver Nanoparticles Preparation. Materials (Basel). 2019; 13(1). PMC: 6981963. DOI: 10.3390/ma13010001. View

Wolf M, Daly K, Brennan-Pierce E, Johnson S, Carruthers C, DAmore A . A hydrogel derived from decellularized dermal extracellular matrix. Biomaterials. 2012; 33(29):7028-38. PMC: 3408574. DOI: 10.1016/j.biomaterials.2012.06.051. View

Sellaro T, Ranade A, Faulk D, McCabe G, Dorko K, Badylak S . Maintenance of human hepatocyte function in vitro by liver-derived extracellular matrix gels. Tissue Eng Part A. 2009; 16(3):1075-82. PMC: 2863084. DOI: 10.1089/ten.TEA.2008.0587. View

Siah C, Childs C, Chia C, Cheng K . An observational study of temperature and thermal images of surgical wounds for detecting delayed wound healing within four days after surgery. J Clin Nurs. 2019; 28(11-12):2285-2295. DOI: 10.1111/jocn.14832. View

Kobayashi H, Kosaka T, Mikami S, Kimura T, Hongo H, Kosugi M . Vasohibin-1 expression as a biomarker of aggressive nature in ductal adenocarcinoma of the prostate: a retrospective cohort study at two centres in Japan. BMJ Open. 2021; 11(11):e056439. PMC: 8614138. DOI: 10.1136/bmjopen-2021-056439. View

Perez-Diaz M, Alvarado-Gomez E, Magana-Aquino M, Sanchez-Sanchez R, Velasquillo C, Gonzalez C . Anti-biofilm activity of chitosan gels formulated with silver nanoparticles and their cytotoxic effect on human fibroblasts. Mater Sci Eng C Mater Biol Appl. 2015; 60:317-323. DOI: 10.1016/j.msec.2015.11.036. View

10.

Reategui-Pinedo N, Salirrosas D, Sanchez-Tuesta L, Quinones C, Jauregui-Rosas S, Barraza G . Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of (Tilapia) Skin in Young and Old Adults. Molecules. 2022; 27(3). PMC: 8838504. DOI: 10.3390/molecules27031123. View

11.

Chen X, Zhang H, Yang X, Zhang W, Jiang M, Wen T . Preparation and Application of Quaternized Chitosan- and AgNPs-Base Synergistic Antibacterial Hydrogel for Burn Wound Healing. Molecules. 2021; 26(13). PMC: 8271850. DOI: 10.3390/molecules26134037. View

12.

Paramelle D, Sadovoy A, Gorelik S, Free P, Hobley J, Fernig D . A rapid method to estimate the concentration of citrate capped silver nanoparticles from UV-visible light spectra. Analyst. 2014; 139(19):4855-61. DOI: 10.1039/c4an00978a. View

13.

Tonnesen M, Feng X, Clark R . Angiogenesis in wound healing. J Investig Dermatol Symp Proc. 2001; 5(1):40-6. DOI: 10.1046/j.1087-0024.2000.00014.x. View

14.

Ardan T, cejkova J . Immunohistochemical expression of matrix metalloproteinases in the rabbit corneal epithelium upon UVA and UVB irradiation. Acta Histochem. 2011; 114(6):540-6. DOI: 10.1016/j.acthis.2011.10.004. View

15.

Pan H, Fan D, Duan Z, Zhu C, Fu R, Li X . Non-stick hemostasis hydrogels as dressings with bacterial barrier activity for cutaneous wound healing. Mater Sci Eng C Mater Biol Appl. 2019; 105:110118. DOI: 10.1016/j.msec.2019.110118. View

16.

Piao M, Kang K, Lee I, Kim H, Kim S, Choi J . Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol Lett. 2010; 201(1):92-100. DOI: 10.1016/j.toxlet.2010.12.010. View

17.

Yang K, Han Q, Chen B, Zheng Y, Zhang K, Li Q . Antimicrobial hydrogels: promising materials for medical application. Int J Nanomedicine. 2018; 13:2217-2263. PMC: 5905846. DOI: 10.2147/IJN.S154748. View

18.

Cai D, Chen S, Wu B, Chen J, Tao D, Li Z . Construction of multifunctional porcine acellular dermal matrix hydrogel blended with vancomycin for hemorrhage control, antibacterial action, and tissue repair in infected trauma wounds. Mater Today Bio. 2021; 12:100127. PMC: 8452890. DOI: 10.1016/j.mtbio.2021.100127. View

19.

El-Sherbiny G, El-Nour S, Askar A, Mohammad N, Hammad A . Solar radiation-induced synthesis of bacterial cellulose/silver nanoparticles (BC/AgNPs) composite using BC as reducing and capping agent. Bioprocess Biosyst Eng. 2021; 45(2):257-268. DOI: 10.1007/s00449-021-02655-y. View

20.

Riaz M, Mutreja V, Sareen S, Ahmad B, Faheem M, Zahid N . Exceptional antibacterial and cytotoxic potency of monodisperse greener AgNPs prepared under optimized pH and temperature. Sci Rep. 2021; 11(1):2866. PMC: 7858571. DOI: 10.1038/s41598-021-82555-z. View