» Articles » PMID: 32065781

LncRNA MEG3 Targeting MiR-424-5p Via MAPK Signaling Pathway Mediates Neuronal Apoptosis in Ischemic Stroke

Overview
Specialty Geriatrics
Date 2020 Feb 18
PMID 32065781
Citations 36
Authors
Affiliations
Soon will be listed here.
Abstract

Emerging evidence suggests that long non-coding RNAs (lncRNAs) are significant regulators in the pathological process of ischemic stroke (IS). However, little is known about lncRNAs and their roles in IS. In this study, we aimed to screen out differentially expressed lncRNAs and revealed the underlying mechanisms in IS. The results of bioinformatic analysis showed that lncRNA MEG3 and were over-expressed in IS samples, while miR-424-5p was lower-expressed. Correlation between MEG3/miR-424-5p, and miR-424-5p/ were predicted with miRanda and TargetScan, and verified by dual luciferase assay. Inhibition of MEG3 remarkably increased the expression of miR-424-5p and decreased the expression of , which also led to in an increased cell viability and decreased cellular apoptosis in oxygen-glucose deprivation and reoxygenation (OGD/R) model, as well as an activated MAPK signaling pathways. Consistently, MEG3 was upregulated in MCAO mice, knockdown of MEG3 reduced the infarct volume and improved neurobehavioral outcomes in rats following MCAO. In conclusion, it was demonstrated that MEG3 accelerated the process of IS by suppressing miR-424-5p, which targeted and the activated MAPK pathway. These results might provide useful information for exploring the potential therapeutic targets in IS.

Citing Articles

Advances in the detection of biomarkers for ischemic stroke.

Liang Y, Chen J, Chen Y, Tong Y, Li L, Xu Y Front Neurol. 2025; 16:1488726.

PMID: 40066310 PMC: 11891058. DOI: 10.3389/fneur.2025.1488726.


LncRNAs Orchestrating Neuroinflammation: A Comprehensive Review.

Esmaeili A, Yazdanpanah N, Rezaei N Cell Mol Neurobiol. 2025; 45(1):21.

PMID: 40056236 PMC: 11890384. DOI: 10.1007/s10571-025-01538-0.


Piperine attenuates cancer-associated pain induced by microglial activation via increasing miR-150-50p.

Chen Y, Wu M Aging (Albany NY). 2024; 16(21):13288-13303.

PMID: 39641645 PMC: 11719110. DOI: 10.18632/aging.205908.


Ischemic Stroke and Autophagy: The Roles of Long Non-Coding RNAs.

Ouyang L, Xia W, Al-Alwany A, Gupta R, Sapaev I, Almalki S Curr Neuropharmacol. 2024; 23(1):85-97.

PMID: 39021183 PMC: 11519825. DOI: 10.2174/1570159X22666240704123701.


Long non-coding RNA MEG3 knockdown represses airway smooth muscle cells proliferation and migration via sponging miR-143-3p/FGF9 in asthma.

Gu J, Zhou D J Cardiothorac Surg. 2024; 19(1):314.

PMID: 38824534 PMC: 11143653. DOI: 10.1186/s13019-024-02798-5.


References
1.
Li Z, Yang L, Liu X, Wang X, Pan Y, Luo J . Corrigendum to "the long noncoding RNA MEG3 and its target miR-147 regulate JAK/STAT pathway in advanced chronic myeloid leukemia" [EBioMedicine 35 (2018) 61-75]. EBioMedicine. 2018; 37:569. PMC: 6286865. DOI: 10.1016/j.ebiom.2018.10.049. View

2.
Shukla G, Singh J, Barik S . MicroRNAs: Processing, Maturation, Target Recognition and Regulatory Functions. Mol Cell Pharmacol. 2012; 3(3):83-92. PMC: 3315687. View

3.
Yang M, Wang X, Fan Y, Chen Y, Sun D, Xu X . Semaphorin 3A Contributes to Secondary Blood-Brain Barrier Damage After Traumatic Brain Injury. Front Cell Neurosci. 2019; 13:117. PMC: 6444306. DOI: 10.3389/fncel.2019.00117. View

4.
De Mariano M, Stigliani S, Moretti S, Parodi F, Croce M, Bernardi C . A genome-wide microRNA profiling indicates miR-424-5p and miR-503-5p as regulators of ALK expression in neuroblastoma. Oncotarget. 2017; 8(34):56518-56532. PMC: 5593579. DOI: 10.18632/oncotarget.17033. View

5.
Liu J, Gu Z, Tang Y, Hao J, Zhang C, Yang X . Tumour-suppressive microRNA-424-5p directly targets CCNE1 as potential prognostic markers in epithelial ovarian cancer. Cell Cycle. 2017; 17(3):309-318. PMC: 5914728. DOI: 10.1080/15384101.2017.1407894. View