Hypoxia, a Dynamic Tool to Amplify the Gingival Mesenchymal Stem Cells Potential for Neurotrophic Factor Secretion

Overview

Journal Saudi J Biol Sci

Specialty Biology

Date 2022 Jul 18

PMID 35844419

Authors

Shankargouda Patil

Hytham N Fageeh

Hammam Ibrahim Fageeh

Wael Ibraheem

Abdulrahman Saleh Alshehri

Ashraf Al-Brakati

Salem Almoammar

Mohammad Almagbol

Harisha Dewan

Samar Saeed Khan

Hosam Ali Baeshen

Vikrant R Patil

A Thirumal Raj

Shilpa Bhandi

Affiliations

Soon will be listed here.

Abstract

Gingival mesenchymal stem cells (GMSCs) have significant regenerative potential. Their potential applications range from the treatment of inflammatory diseases, wound healing, and oral disorders. Preconditioning these stem cells can optimize their biological properties. Hypoxia preconditioning of MSCs improves stem cell properties like proliferation, survival, and differentiation potential. This research explored the possible impact of hypoxia on the pluripotent stem cell properties that GMSCs possess. We evaluated the morphology, stemness, neurotrophic factors, and stemness-related genes. We compared the protein levels of secreted neurotrophic factors between normoxic and hypoxic GMSC-conditioned media (GMSC-CM). Results revealed that hypoxic cultured GMSC's had augmented expression of neurotrophic factors BDNF, GDNF, VEGF, and IGF1 and stemness-related gene NANOG. Hypoxic GMSCs showed decreased expression of the OCT4 gene. In hypoxic GMSC-CM, the neurotrophic factors secretions were significantly higher than normoxic GMSC-CM. Our data demonstrate that culturing of GMSCs in hypoxia enhances the secretion of neurotrophic factors that can lead to neuronal lineage differentiation.

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