Neutrophils Inhibit CD8 T Cells Immune Response by Arginase-1 Signaling in Patients with Sepsis

Overview

Journal World J Emerg Med

Specialty Emergency Medicine

Date 2022 Jul 15

PMID 35837557

Authors

Xiao-Kang Dai

Zhen-Xing Ding

Yuan-Yuan Tan

Hua-Rui Bao

Dong-Yao Wang

Hong Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Patients with sepsis often exhibit an acute inflammatory response, followed by an immunosuppressive phase with a poor immune response. However, the underlying mechanisms have not been fully elucidated.

Methods: We sought to comprehensively characterize the transcriptional changes in neutrophils of patients with sepsis by transcriptome sequencing. Additionally, we conducted a series of experiments, including real-time quantitative polymerase chain reaction (RT-qPCR) and flow cytometry to investigate the role of arginase-1 signaling in sepsis.

Results: Through the analysis of gene expression profiles, we identified that the negative regulation of T cell activation signaling was enriched, and the expression of arginase-1 was high in neutrophils from patients with sepsis. Furthermore, we conducted flow cytometry and found that the function of CD8 T cells in septic patients was impaired. Moreover, neutrophils from septic patients inhibited the percentage of polyfunctional effector CD8 T cells through arginase-1. Additionally, the proportions of granzyme BIFNγCD8 T and TNFαIFNγCD8 T cells increased after inhibition of arginase-1 signaling.

Conclusion: The impaired effector function of CD8 T cells could be restored by blocking arginase-1 signaling in patients with sepsis.

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