A Systematic Review of Oral Biopsies, Sample Types, and Detection Techniques Applied in Relation to Oral Cancer Detection

Overview

Journal BioTech (Basel)

Specialty Biotechnology

Date 2022 Jul 13

PMID 35822813

Authors

Guanghuan Yang

Luqi Wei

Benjamin K S Thong

Yuanyuan Fu

Io Hong Cheong

Zisis Kozlakidis

Xue Li

Hui Wang

Xiaoguang Li

Affiliations

Soon will be listed here.

Abstract

Early identification of the stage of oral cancer development can lead to better treatment outcomes and avoid malignant transformation. Therefore, this review aims to provide a comprehensive overview that describes the development of standardized procedures for oral sample collection, characterization, and molecular risk assessment. This can help investigators to choose the appropriate sampling method and downstream analyses for different purposes. This systematic review was conducted according to the PRISMA guidelines. Using both PubMed and Web of Science databases, four independent authors conducted a literature search between 15 and 21 June 2021. We used key search terms to broaden the search for studies. Non-conforming articles were removed using an EndNote-based and manual approach. Reviewers used a designed form to extract data. This review included a total of 3574 records, after eliminating duplicate articles and excluding papers that did not meet the inclusion criteria. Finally, 202 articles were included in this review. We summarized the sampling methods, biopsy samples, and downstream analysis. The biopsy techniques were classified into tissue and liquid biopsy. The common sequential analysis of tissue biopsy includes histopathological examination such as H&E or IHC to identify various pathogenic features. Meanwhile, liquid samples such as saliva, blood, and urine are analyzed for the purpose of screening to detect mutations in cancer. Commonly used technologies are PCR, RT-PCR, high-throughput sequencing, and metabolomic analysis. Conclusions: Currently, tissue biopsies provide increased diagnostic value compared to liquid biopsy. However, the minimal invasiveness and convenience of liquid biopsy make it a suitable method for mass screening and eventual clinical adoption. The analysis of samples includes histological and molecular analysis. Metabolite analysis is rising but remains scarce.

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References

cankovic M, Ilic M, Vuckovic N, Bokor-Bratic M . The histological characteristics of clinically normal mucosa adjacent to oral cancer. J Cancer Res Ther. 2013; 9(2):240-4. DOI: 10.4103/0973-1482.113365. View

Mehrotra R, Singh M, Pandya S, Singh M . The use of an oral brush biopsy without computer-assisted analysis in the evaluation of oral lesions: a study of 94 patients. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008; 106(2):246-53. DOI: 10.1016/j.tripleo.2008.02.030. View

Ren Z, Hu C, He H, Li Y, Lyu J . Global and regional burdens of oral cancer from 1990 to 2017: Results from the global burden of disease study. Cancer Commun (Lond). 2020; 40(2-3):81-92. PMC: 7163731. DOI: 10.1002/cac2.12009. View

Werner J, Dunne A, Ramaswamy A, Folz B, Brandt D, Kulkens C . Number and location of radiolabeled, intraoperatively identified sentinel nodes in 48 head and neck cancer patients with clinically staged N0 and N1 neck. Eur Arch Otorhinolaryngol. 2002; 259(2):91-6. DOI: 10.1007/s00405-001-0421-2. View

Paderni C, Compilato D, Carinci F, Nardi G, Rodolico V, Lo Muzio L . Direct visualization of oral-cavity tissue fluorescence as novel aid for early oral cancer diagnosis and potentially malignant disorders monitoring. Int J Immunopathol Pharmacol. 2011; 24(2 Suppl):121-8. DOI: 10.1177/03946320110240S221. View

Naugler C . Practice tips. Brush biopsy sampling of oral lesions. Can Fam Physician. 2008; 54(2):194. PMC: 2278308. View

Remmerbach T, Weidenbach H, Muller C, Hemprich A, Pomjanski N, Buckstegge B . Diagnostic value of nucleolar organizer regions (AgNORs) in brush biopsies of suspicious lesions of the oral cavity. Anal Cell Pathol. 2003; 25(3):139-46. PMC: 4618969. DOI: 10.1155/2003/647685. View

de Koning K, Koppes S, de Bree R, Dankbaar J, Willems S, van Es R . Feasibility study of ultrasound-guided resection of tongue cancer with immediate specimen examination to improve margin control - Comparison with conventional treatment. Oral Oncol. 2021; 116:105249. DOI: 10.1016/j.oraloncology.2021.105249. View

Park W, Jin H, Heo Y, Jeong H, Son Y, Chung M . Sentinel Lymph Node Biopsy Versus Elective Neck Dissection: Long-Term Oncologic Outcomes in Clinically Node-Negative Tongue Cancer. Clin Exp Otorhinolaryngol. 2021; 15(1):107-114. PMC: 8901953. DOI: 10.21053/ceo.2020.02411. View

10.

Dunn R, DEVINE K . Tetracycline-induced fluorescence of laryngeal, pharyngeal, and oral cancer. Laryngoscope. 1972; 82(2):189-98. DOI: 10.1288/00005537-197202000-00005. View

11.

Drinnan A . Screening for oral cancer and precancer--a valuable new technique. Gen Dent. 2002; 48(6):656-60. View

12.

Momen-Heravi F, Trachtenberg A, Kuo W, Cheng Y . Genomewide Study of Salivary MicroRNAs for Detection of Oral Cancer. J Dent Res. 2014; 93(7 Suppl):86S-93S. PMC: 4107544. DOI: 10.1177/0022034514531018. View

13.

Kovacs A, Dobert N, Walendzik H, Zaplatnikov K, Landes C . The diagnostic role of radioactivity in sentinel nodes in oral and oropharyngeal cancer. Cancer Biother Radiopharm. 2006; 21(5):535-43. DOI: 10.1089/cbr.2006.21.535. View

14.

Velleuer E, Dietrich R, Pomjanski N, de Santana Almeida Araujo I, Silva de Araujo B, Sroka I . Diagnostic accuracy of brush biopsy-based cytology for the early detection of oral cancer and precursors in Fanconi anemia. Cancer Cytopathol. 2020; 128(6):403-413. DOI: 10.1002/cncy.22249. View

15.

Ishiguro K, Iwai T, Izumi T, Sugiyama S, Baba J, Oguri S . Sentinel lymph node biopsy with preoperative CT lymphography and intraoperative indocyanine green fluorescence imaging for N0 early tongue cancer: A long-term follow-up study. J Craniomaxillofac Surg. 2020; 48(3):217-222. DOI: 10.1016/j.jcms.2020.01.007. View

16.

Chone C, Aniteli M, Magalhaes R, Freitas L, Altemani A, Ramos C . Impact of immunohistochemistry in sentinel lymph node biopsy in head and neck cancer. Eur Arch Otorhinolaryngol. 2012; 270(1):313-7. DOI: 10.1007/s00405-012-2032-5. View

17.

Sokolov K, Nieman L, Myakov A, Gillenwater A . Polarized reflectance spectroscopy for pre-cancer detection. Technol Cancer Res Treat. 2004; 3(1):1-14. DOI: 10.1177/153303460400300101. View

18.

Flach G, Tenhagen M, de Bree R, Brakenhoff R, van der Waal I, Bloemena E . Outcome of patients with early stage oral cancer managed by an observation strategy towards the N0 neck using ultrasound guided fine needle aspiration cytology: No survival difference as compared to elective neck dissection. Oral Oncol. 2012; 49(2):157-64. DOI: 10.1016/j.oraloncology.2012.08.006. View

19.

Perera M, Al-Hebshi N, Perera I, Ipe D, Ulett G, Speicher D . A dysbiotic mycobiome dominated by is identified within oral squamous-cell carcinomas. J Oral Microbiol. 2017; 9(1):1385369. PMC: 5678454. DOI: 10.1080/20002297.2017.1385369. View

20.

Sagheb K, Sagheb K, Rahimi-Nedjat R, Taylor K, Al-Nawas B, Walter C . Sentinel lymph node biopsy in T1/T2 squamous cell carcinomas of the tongue: A prospective study. Oncol Lett. 2016; 11(1):600-604. PMC: 4727194. DOI: 10.3892/ol.2015.3933. View