A Dysbiotic Mycobiome Dominated by is Identified Within Oral Squamous-cell Carcinomas

Overview

Journal J Oral Microbiol

Specialty Dentistry

Date 2017 Nov 21

PMID 29152157

Citations 45

Authors

Manosha Perera

Nezar Noor Al-Hebshi

Irosha Perera

Deepak Ipe

Glen C Ulett

David J Speicher

Tsute Chen

Newell W Johnson

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to characterize the mycobiome associated with oral squamous-cell carcinoma (OSCC). DNA was extracted from 52 tissue biopsies (cases: 25 OSCC; controls: 27 intra-oral fibro-epithelial polyps [FEP]) and sequenced for the fungal internal transcribed spacer 2 region using Illumina™ 2 x300bp chemistry. Merged reads were classified to species level using a BLASTN-algorithm with UNITE's named species sequences as reference. Downstream analyses were performed using QIIME™ and linear discriminant analysis effect size. A total of 364 species representing 160 genera and two phyla (Ascomycota and Basidiomycota) were identified, with and making up 48% and 11% of the average mycobiome, respectively. However, only five species and four genera were detected in ≥50% of the samples. The species richness and diversity were significantly lower in OSCC. Genera , , and were overrepresented in OSCC; and were more abundant in FEP. Species-wise, , , and a -like species were enriched in OSCC, while -like species, , and were the most significantly abundant in FEP. In conclusion, a dysbiotic mycobiome dominated by was found in association with OSCC, a finding worth further investigation.

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