Cell-penetrating Peptide for Targeted Macromolecule Delivery into Plant Chloroplasts

Overview

Journal Appl Microbiol Biotechnol

Specialties Biomedical Engineering
Biotechnology
Microbiology

Date 2022 Jul 13

PMID 35821432

Authors

Vivek Kumar

Archana Chugh

Affiliations

Soon will be listed here.

Abstract

Reports on chloroplast-targeted protein delivery using cell-penetrating peptides are scarce. In this study, a novel peptide-based macromolecule delivery strategy targeting chloroplasts was successfully developed in wheat mesophyll protoplasts. A peptide derived from the signal sequence of the chloroplast-targeted protein of ferredoxin-thioredoxin reductase catalytic chain of Spinacia oleracea with UniProtKB Id-P41348 exhibits properties of cellular internalization. DNase I was efficiently delivered into the chloroplast using 10 μM cTP with an efficiency of more than 90%. This cell-penetrating peptide-mediated approach offers various advantages over the existing chloroplast targeting methods, such as non-invasiveness, biocompatibility, low-toxicity, and target-specific delivery. The present study shows that peptide-based strategies hold tremendous potential in the field of chloroplast biotechnology. KEY POINTS: • Screening of database of chloroplast targeting peptides in order to develop an efficient cell-penetrating peptide termed as cTP. • cTP efficiently crosses the cell barrier and demonstrated chloroplast-localization. • cTP can be incorporated as a promising strategy for delivering macromolecules for crop improvement.

Citing Articles

Membrane-acting biomimetic peptoids against visceral leishmaniasis.

Kumar V, Lin J, Molchanova N, Fortkort J, Reckmann C, Brase S FEBS Open Bio. 2023; 13(3):519-531.

PMID: 36683396 PMC: 9989931. DOI: 10.1002/2211-5463.13562.

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