Circ_0110940 Exerts an Antiapoptotic and Pro-Proliferative Effect in Gastric Cancer Cells Via the MiR-1178-3p/SLC38A6 Axis

Overview

Journal J Oncol

Specialty Oncology

Date 2022 Jul 11

PMID 35813866

Authors

Xiaonan Miao

Haiou Zou

Lijuan Pan

Jing Cheng

Youshan Wu

Rui Chen

Yong Su

Hongwei Du

Affiliations

Soon will be listed here.

Abstract

Circular RNAs (circRNAs) are essential regulators in human cancers, including gastric cancer, by the miRNA/mRNA axis. A previous study identified the upregulation of circ_0110940 in human gastric cancer tissues. The present study performed assays to reveal the functions of circ_0110940 and its downstream miRNA/mRNA axis in gastric cancer cells. Traditional proliferation and apoptosis assays including colony formation, EdU staining, and Annexin V-PI staining assays were conducted. A luciferase reporter assay was performed to assess the binding between miR-1178-3p and circ_0110940 or SLC38A. We found the significant upregulation of circ_0110940 in human gastric cancer cells AGS and MKN45. Circ_0110940 was a stable circRNA and exerted an antiproliferative and proapoptotic effect in AGS and MKN45. Circ_0110940 binded with miR-1178-3p, which further targeted SLC38A6 3'UTR. Circ_0110940 degraded miR-1178-3p, and miR-1178-3p degraded SLC38A6. Thus, circ_0110940 has a positive effect on SLC38A6 expression. Furthermore, SLC38A6 rescued the effects of circ_0110940 knockdown on gastric cancer cell proliferation and apoptosis. In conclusion, circ_0110940 exerted an antiapoptotic and pro-proliferative effect in gastric cancer cells via the miR-1178-3p/SLC38A6 axis, which may provide basis for the targeted therapy of gastric cancer.

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References

Glazar P, Papavasileiou P, Rajewsky N . circBase: a database for circular RNAs. RNA. 2014; 20(11):1666-70. PMC: 4201819. DOI: 10.1261/rna.043687.113. View

Tang Z, Li C, Kang B, Gao G, Li C, Zhang Z . GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 2017; 45(W1):W98-W102. PMC: 5570223. DOI: 10.1093/nar/gkx247. View

Sung H, Ferlay J, Siegel R, Laversanne M, Soerjomataram I, Jemal A . Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3):209-249. DOI: 10.3322/caac.21660. View

Cao J, Zhang X, Xu P, Wang H, Wang S, Zhang L . Circular RNA circLMO7 acts as a microRNA-30a-3p sponge to promote gastric cancer progression via the WNT2/β-catenin pathway. J Exp Clin Cancer Res. 2021; 40(1):6. PMC: 7784001. DOI: 10.1186/s13046-020-01791-9. View

Farkona S, Diamandis E, Blasutig I . Cancer immunotherapy: the beginning of the end of cancer?. BMC Med. 2016; 14:73. PMC: 4858828. DOI: 10.1186/s12916-016-0623-5. View

Liao Y, Feng Y, Shen J, Hornicek F, Duan Z . The roles and therapeutic potential of cyclin-dependent kinases (CDKs) in sarcoma. Cancer Metastasis Rev. 2015; 35(2):151-63. DOI: 10.1007/s10555-015-9601-1. View

Gong L, Chen J, Dong M, Xiao Z, Feng Z, Pan Y . Epstein-Barr virus-derived circular RNA LMP2A induces stemness in EBV-associated gastric cancer. EMBO Rep. 2020; 21(10):e49689. PMC: 7534631. DOI: 10.15252/embr.201949689. View

Wang X, Li J, Bian X, Wu C, Hua J, Chang S . interacts with hnRNPM to inhibit metastasis by modulating alternative splicing in gastric cancer. Proc Natl Acad Sci U S A. 2021; 118(33). PMC: 8379983. DOI: 10.1073/pnas.2012881118. View

Kristensen L, Andersen M, Stagsted L, Ebbesen K, Hansen T, Kjems J . The biogenesis, biology and characterization of circular RNAs. Nat Rev Genet. 2019; 20(11):675-691. DOI: 10.1038/s41576-019-0158-7. View

10.

Wang L, Deng A, Zhao L, Li Q, Wang M, Zhang Y . MiR-1178-3p promotes the proliferation, migration and invasion of nasopharyngeal carcinoma Sune-1 cells by targeting STK4. J Biol Regul Homeost Agents. 2019; 33(2):321-330. View

11.

Bang Y, Van Cutsem E, Feyereislova A, Chung H, Shen L, Sawaki A . Trastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2-positive advanced gastric or gastro-oesophageal junction cancer (ToGA): a phase 3, open-label, randomised controlled trial. Lancet. 2010; 376(9742):687-97. DOI: 10.1016/S0140-6736(10)61121-X. View

12.

Bagchi S, Baomar H, Al-Walai S, Al-Sadi S, Fredriksson R . Histological analysis of SLC38A6 (SNAT6) expression in mouse brain shows selective expression in excitatory neurons with high expression in the synapses. PLoS One. 2014; 9(4):e95438. PMC: 3994050. DOI: 10.1371/journal.pone.0095438. View

13.

Lobo R . Hormone-replacement therapy: current thinking. Nat Rev Endocrinol. 2016; 13(4):220-231. DOI: 10.1038/nrendo.2016.164. View

14.

Gandasi N, Arapi V, Mickael M, Belekar P, Granlund L, Kothegala L . Glutamine Uptake via SNAT6 and Caveolin Regulates Glutamine-Glutamate Cycle. Int J Mol Sci. 2021; 22(3). PMC: 7865731. DOI: 10.3390/ijms22031167. View

15.

Dudekula D, Panda A, Grammatikakis I, De S, Abdelmohsen K, Gorospe M . CircInteractome: A web tool for exploring circular RNAs and their interacting proteins and microRNAs. RNA Biol. 2015; 13(1):34-42. PMC: 4829301. DOI: 10.1080/15476286.2015.1128065. View

16.

Tawfik D, Zaccagnino A, Bernt A, Szczepanowski M, Klapper W, Schwab A . The A818-6 system as an in-vitro model for studying the role of the transportome in pancreatic cancer. BMC Cancer. 2020; 20(1):264. PMC: 7106758. DOI: 10.1186/s12885-020-06773-w. View

17.

Fuchs C, Tomasek J, Yong C, Dumitru F, Passalacqua R, Goswami C . Ramucirumab monotherapy for previously treated advanced gastric or gastro-oesophageal junction adenocarcinoma (REGARD): an international, randomised, multicentre, placebo-controlled, phase 3 trial. Lancet. 2013; 383(9911):31-39. DOI: 10.1016/S0140-6736(13)61719-5. View

18.

Guo J, Yang Y, Guo M, Zhang J, Zheng J, Liu Z . Involvement of CDK11B-mediated SPDEF ubiquitination and SPDEF-mediated microRNA-448 activation in the oncogenicity and self-renewal of hepatocellular carcinoma stem cells. Cancer Gene Ther. 2020; 28(10-11):1136-1149. DOI: 10.1038/s41417-020-00261-w. View

19.

Liu H, Bi J, Dong W, Yang M, Shi J, Jiang N . Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis. Mol Cancer. 2018; 17(1):161. PMC: 6245936. DOI: 10.1186/s12943-018-0908-8. View

20.

Alonso M, Ausso S, Lopez-Doriga A, Cordero D, Guino E, Sole X . Comprehensive analysis of copy number aberrations in microsatellite stable colon cancer in view of stromal component. Br J Cancer. 2017; 117(3):421-431. PMC: 5537504. DOI: 10.1038/bjc.2017.208. View