Alternative Molecular Tools for the Fight Against Infectious Diseases of Small Ruminants: Native Sicilian Sheep Breeds and Maedi-Visna Genetic Susceptibility

Overview

Journal Animals (Basel)

Date 2022 Jul 9

PMID 35804527

Authors

Serena Tumino

Marco Tolone

Paola Galluzzo

Sergio Migliore

Tiziana Sechi

Salvatore Bordonaro

Roberto Puleio

Antonello Carta

Guido Ruggero Loria

Affiliations

Soon will be listed here.

Abstract

Maedi-visna (MV) is a disease caused by small ruminant lentiviruses. It is included in the list of notifiable terrestrial animal diseases due to economic losses and animal welfare harm in the sheep sector. To date, control programs remain the onliest approach to avoiding infection. The allelic variant p.Glu35Lys (E35K) of the gene has been strongly associated with host vulnerability to MV illness. The present study aimed to investigate the association of E35K allele frequencies with MV susceptibility in native Sicilian sheep breeds. More than 400 animals from 14 local sheep were serologically tested and genotyped for the E35K polymorphism. The local breeds displayed different values of MV seroprevalence, with the lowest antibody prevalence in Barbaresca and Pinzirita breeds. protective allele (K35) was less frequent than the risk allele (E35) in Valle del Belìce breed, whereas the other three breeds showed a more balanced alleles distribution. A positive association between seroprevalence and genotype was found in the entire sample set. The risk of infection resulted in more than 3-fold times as high in sheep with EK and EE genotype compared to the KK genotype. Our data could be helpful in establishing selection breeding programs aimed at reducing MV infection in Sicilian sheep farming and encouraging the breeding of native breeds.

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References

Arcangeli C, Lucarelli D, Torricelli M, Sebastiani C, Ciullo M, Pellegrini C . First Survey of SNPs in , , and Genes in Sheep Reared in Italy and Their Association with Resistance to SRLVs Infection. Viruses. 2021; 13(7). PMC: 8310241. DOI: 10.3390/v13071290. View

Shah C, Boni J, Huder J, Vogt H, Muhlherr J, Zanoni R . Phylogenetic analysis and reclassification of caprine and ovine lentiviruses based on 104 new isolates: evidence for regular sheep-to-goat transmission and worldwide propagation through livestock trade. Virology. 2004; 319(1):12-26. DOI: 10.1016/j.virol.2003.09.047. View

Heaton M, Clawson M, Chitko-McKown C, Leymaster K, Smith T, Harhay G . Reduced lentivirus susceptibility in sheep with TMEM154 mutations. PLoS Genet. 2012; 8(1):e1002467. PMC: 3266874. DOI: 10.1371/journal.pgen.1002467. View

de Miguel R, Arrieta M, Rodriguez-Largo A, Echeverria I, Resendiz R, Perez E . Worldwide Prevalence of Small Ruminant Lentiviruses in Sheep: A Systematic Review and Meta-Analysis. Animals (Basel). 2021; 11(3). PMC: 8000744. DOI: 10.3390/ani11030784. View

Peterson K, Brinkhof J, Houwers D, Colenbrander B, Gadella B . Presence of pro-lentiviral DNA in male sexual organs and ejaculates of small ruminants. Theriogenology. 2007; 69(4):433-42. DOI: 10.1016/j.theriogenology.2007.10.013. View

Bishop S . Genetic resistance to infections in sheep. Vet Microbiol. 2015; 181(1-2):2-7. DOI: 10.1016/j.vetmic.2015.07.013. View

White S, Mousel M, Reynolds J, Lewis G, Herrmann-Hoesing L . Common promoter deletion is associated with 3.9-fold differential transcription of ovine CCR5 and reduced proviral level of ovine progressive pneumonia virus. Anim Genet. 2009; 40(5):583-9. DOI: 10.1111/j.1365-2052.2009.01882.x. View

Hunter N, Goldmann W, Foster J, Cairns D, Smith G . Natural scrapie and PrP genotype: case-control studies in British sheep. Vet Rec. 1997; 141(6):137-40. DOI: 10.1136/vr.141.6.137. View

Vitale M, Migliore S, La Giglia M, Alberti P, Presti V, Langeveld J . Scrapie incidence and PRNP polymorphisms: rare small ruminant breeds of Sicily with TSE protecting genetic reservoirs. BMC Vet Res. 2016; 12(1):141. PMC: 4946234. DOI: 10.1186/s12917-016-0766-9. View

10.

Arcangeli C, Torricelli M, Sebastiani C, Lucarelli D, Ciullo M, Passamonti F . Genetic Characterization of Small Ruminant Lentiviruses (SRLVs) Circulating in Naturally Infected Sheep in Central Italy. Viruses. 2022; 14(4). PMC: 9032261. DOI: 10.3390/v14040686. View

11.

Alshanbari F, Mousel M, Reynolds J, Herrmann-Hoesing L, Highland M, Lewis G . Mutations in Ovis aries TMEM154 are associated with lower small ruminant lentivirus proviral concentration in one sheep flock. Anim Genet. 2014; 45(4):565-71. PMC: 4140605. DOI: 10.1111/age.12181. View

12.

Minguijon E, Reina R, Perez M, Polledo L, Villoria M, Ramirez H . Small ruminant lentivirus infections and diseases. Vet Microbiol. 2015; 181(1-2):75-89. DOI: 10.1016/j.vetmic.2015.08.007. View

13.

Leymaster K, Chitko-McKown C, Clawson M, Harhay G, Heaton M . Effects of TMEM154 haplotypes 1 and 3 on susceptibility to ovine progressive pneumonia virus following natural exposure in sheep. J Anim Sci. 2013; 91(11):5114-21. DOI: 10.2527/jas.2013-6663. View

14.

Sider L, Heaton M, Chitko-McKown C, Harhay G, Smith T, Leymaster K . Small ruminant lentivirus genetic subgroups associate with sheep TMEM154 genotypes. Vet Res. 2013; 44:64. PMC: 3734121. DOI: 10.1186/1297-9716-44-64. View

15.

Straub O . Maedi-Visna virus infection in sheep. History and present knowledge. Comp Immunol Microbiol Infect Dis. 2003; 27(1):1-5. DOI: 10.1016/S0147-9571(02)00078-4. View

16.

Gayo E, Polledo L, Balseiro A, Perez Martinez C, Garcia Iglesias M, Preziuso S . Inflammatory Lesion Patterns in Target Organs of Visna/Maedi in Sheep and their Significance in the Pathogenesis and Diagnosis of the Infection. J Comp Pathol. 2018; 159:49-56. DOI: 10.1016/j.jcpa.2018.01.001. View

17.

Kalogianni A, Bossis I, Ekateriniadou L, Gelasakis A . Etiology, Epizootiology and Control of Maedi-Visna in Dairy Sheep: A Review. Animals (Basel). 2020; 10(4). PMC: 7222820. DOI: 10.3390/ani10040616. View

18.

White S, Knowles D . Expanding possibilities for intervention against small ruminant lentiviruses through genetic marker-assisted selective breeding. Viruses. 2013; 5(6):1466-99. PMC: 3717717. DOI: 10.3390/v5061466. View

19.

Gerstner S, Adamovicz J, Duncan J, Laegreid W, Marshall K, Logan J . Prevalence of and risk factors associated with ovine progressive pneumonia in Wyoming sheep flocks. J Am Vet Med Assoc. 2015; 247(8):932-7. DOI: 10.2460/javma.247.8.932. View

20.

Murphy T, Chitko-McKown C, Heaton M, Freking B . Effect of TMEM154 E35K variant (haplotypes 1 and 3) on the incidence of ovine lentivirus infection and ewe productivity during lifetime exposure. J Anim Sci. 2021; 99(11). PMC: 8562468. DOI: 10.1093/jas/skab304. View