Positive Effects of Phycocyanobilin on Gene Expression in Glutamate-induced Excitotoxicity in SH-SY5Y Cells and Animal Models of Multiple Sclerosis and Cerebral Ischemia

Overview

Journal Heliyon

Specialty Social Sciences

Date 2022 Jul 8

PMID 35800718

Authors

Daniel Palenzuela Gardon

Majel Cervantes-Llanos

Beatriz Piniella Matamoros

Hanlet Camacho Rodriguez

Chan-Yuan Tan

Javier Marin-Prida

Viviana Falcon-Cama

Nancy Pavon-Fuentes

Jessica Gomez Lemus

Laura de la Caridad Bakos Ruiz

Tamara Diaz Argudin

Gillian Martinez Donato

Yasser Perera

Ke Yang

Giselle Penton-Rol

Affiliations

Soon will be listed here.

Abstract

Background: Oxidative stress has a predominant role in the pathogenesis of neurodegenerative diseases and therefore the modulation of genes and the identification of biological pathways associated with antioxidant therapies, have an impact on its treatment.

Objective: The objective of this study was the comparison of 2 methods for the analysis of real-time PCR (qPCR) data, through the use of the evaluation of genes that mediate the effect of Phycocyanobilin (PCB) and its validation in animal models.

Methods: We evaluated the effect of PCB:" in vitro" on gene modulation through qPCR analyzed by parametric ANOVA and multivariate principal component analysis (PCA) in a model of glutamate-induced excitotoxicity in the SH-SY5Y cell line and" in vivo"; in animal models of multiple sclerosis (MS) and cerebral ischemia (CI).

Results: The results showed that PCA is a robust and powerful method that allows the assessment of gene expression profiles. We detected the significant down-regulation of the (NOX2), and HMOX1 by the action of PCB in SH-5YSH cell line insulted with Glutamate. The decrease in pro-inflammatory cytokines and markers related to apoptosis and innate immune response, mediated the effect of PCB in the animal models of MS and CI, respectively.

Conclusion: We concluded that the mechanisms by which PCB protected cells included the reduction of oxidative stress damage, which could contribute to its clinical efficacy for the treatment of neurodegenerative diseases.

Citing Articles

Antioxidant Therapies in the Treatment of Multiple Sclerosis.

Jimenez-Jimenez F, Alonso-Navarro H, Salgado-Camara P, Garcia-Martin E, Agundez J Biomolecules. 2024; 14(10).

PMID: 39456199 PMC: 11506420. DOI: 10.3390/biom14101266.

Isobolographic Analysis of the Cytoprotective Effect of Dapsone and Cannabidiol Alone or Combination upon Oxygen-Glucose Deprivation/Reoxygenation Model in SH-SY5Y Cells.

Islas-Cortez M, Rios C, Manzanares J, Diaz-Ruiz A, Perez-Pasten-Borja R Antioxidants (Basel). 2024; 13(6).

PMID: 38929144 PMC: 11200396. DOI: 10.3390/antiox13060705.

Role of Natural Compounds Modulating Heme Catabolic Pathway in Gut, Liver, Cardiovascular, and Brain Diseases.

Jayanti S, Vitek L, Verde C, Llido J, Sukowati C, Tiribelli C Biomolecules. 2024; 14(1).

PMID: 38254662 PMC: 10813662. DOI: 10.3390/biom14010063.

The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome.

Marin-Prida J, Rodriguez-Ulloa A, Besada V, Llopiz-Arzuaga A, Batista N, Hernandez-Gonzalez I Front Immunol. 2023; 14:1227268.

PMID: 37936684 PMC: 10627171. DOI: 10.3389/fimmu.2023.1227268.

Stalling the Course of Neurodegenerative Diseases: Could Cyanobacteria Constitute a New Approach toward Therapy?.

Ramos V, Reis M, Ferreira L, Silva A, Ferraz R, Vieira M Biomolecules. 2023; 13(10).

PMID: 37892126 PMC: 10604708. DOI: 10.3390/biom13101444.

References

Lech M, Anders H . Expression profiling by real-time quantitative polymerase chain reaction (RT-qPCR). Methods Mol Biol. 2014; 1169:133-42. DOI: 10.1007/978-1-4939-0882-0_13. View

Gupta A, Lacoste B, Pistell P, Pistel P, Ingram D, Hamel E . Neurotherapeutic effects of novel HO-1 inhibitors in vitro and in a transgenic mouse model of Alzheimer's disease. J Neurochem. 2014; 131(6):778-90. DOI: 10.1111/jnc.12927. View

Nitti M, Piras S, Brondolo L, Marinari U, Pronzato M, Furfaro A . Heme Oxygenase 1 in the Nervous System: Does It Favor Neuronal Cell Survival or Induce Neurodegeneration?. Int J Mol Sci. 2018; 19(8). PMC: 6121636. DOI: 10.3390/ijms19082260. View

Penton-Rol G, Cervantes-Llanos M . Report on the Symposium "Molecular Mechanisms Involved in Neurodegeneration". Behav Sci (Basel). 2018; 8(1). PMC: 5791034. DOI: 10.3390/bs8010016. View

Pavon-Fuentes N, Marin-Prida J, Llopiz-Arzuaga A, Falcon-Cama V, Campos-Mojena R, Cervantes-Llanos M . Phycocyanobilin reduces brain injury after endothelin-1- induced focal cerebral ischaemia. Clin Exp Pharmacol Physiol. 2019; 47(3):383-392. DOI: 10.1111/1440-1681.13214. View

Lingappan K . NF-κB in Oxidative Stress. Curr Opin Toxicol. 2018; 7:81-86. PMC: 5978768. DOI: 10.1016/j.cotox.2017.11.002. View

Jolliffe I, Cadima J . Principal component analysis: a review and recent developments. Philos Trans A Math Phys Eng Sci. 2016; 374(2065):20150202. PMC: 4792409. DOI: 10.1098/rsta.2015.0202. View

Penton-Rol G, Marin-Prida J, McCarty M . C-Phycocyanin-derived Phycocyanobilin as a Potential Nutraceutical Approach for Major Neurodegenerative Disorders and COVID-19- induced Damage to the Nervous System. Curr Neuropharmacol. 2021; 19(12):2250-2275. PMC: 9185767. DOI: 10.2174/1570159X19666210408123807. View

Penton-Rol G, Marin-Prida J, Falcon-Cama V . C-Phycocyanin and Phycocyanobilin as Remyelination Therapies for Enhancing Recovery in Multiple Sclerosis and Ischemic Stroke: A Preclinical Perspective. Behav Sci (Basel). 2018; 8(1). PMC: 5791033. DOI: 10.3390/bs8010015. View

10.

Liao G, Gao B, Gao Y, Yang X, Cheng X, Ou Y . Phycocyanin Inhibits Tumorigenic Potential of Pancreatic Cancer Cells: Role of Apoptosis and Autophagy. Sci Rep. 2016; 6:34564. PMC: 5046139. DOI: 10.1038/srep34564. View

11.

Radi E, Formichi P, Battisti C, Federico A . Apoptosis and oxidative stress in neurodegenerative diseases. J Alzheimers Dis. 2014; 42 Suppl 3:S125-52. DOI: 10.3233/JAD-132738. View

12.

Rodriguez-Esteban R, Jiang X . Differential gene expression in disease: a comparison between high-throughput studies and the literature. BMC Med Genomics. 2017; 10(1):59. PMC: 5637346. DOI: 10.1186/s12920-017-0293-y. View

13.

Bae Y, Oh H, Rhee S, Yoo Y . Regulation of reactive oxygen species generation in cell signaling. Mol Cells. 2011; 32(6):491-509. PMC: 3887685. DOI: 10.1007/s10059-011-0276-3. View

14.

Sun Z, Zhang L, Zhu S, Chen W, Mei B . Excitotoxicity effects of glutamate on human neuroblastoma SH-SY5Y cells via oxidative damage. Neurosci Bull. 2010; 26(1):8-16. PMC: 5560379. DOI: 10.1007/s12264-010-0813-7. View

15.

Riss J, Decorde K, Sutra T, Delage M, Baccou J, Jouy N . Phycobiliprotein C-phycocyanin from Spirulina platensis is powerfully responsible for reducing oxidative stress and NADPH oxidase expression induced by an atherogenic diet in hamsters. J Agric Food Chem. 2007; 55(19):7962-7. DOI: 10.1021/jf070529g. View

16.

Zheng J, Inoguchi T, Sasaki S, Maeda Y, McCarty M, Fujii M . Phycocyanin and phycocyanobilin from Spirulina platensis protect against diabetic nephropathy by inhibiting oxidative stress. Am J Physiol Regul Integr Comp Physiol. 2012; 304(2):R110-20. DOI: 10.1152/ajpregu.00648.2011. View

17.

Martindale J, Holbrook N . Cellular response to oxidative stress: signaling for suicide and survival. J Cell Physiol. 2002; 192(1):1-15. DOI: 10.1002/jcp.10119. View

18.

Chengalvala M, Chennathukuzhi V, Johnston D, Stevis P, Kopf G . Gene expression profiling and its practice in drug development. Curr Genomics. 2008; 8(4):262-70. PMC: 2430683. DOI: 10.2174/138920207781386942. View

19.

Laurell H, Iacovoni J, Abot A, Svec D, Maoret J, Arnal J . Correction of RT-qPCR data for genomic DNA-derived signals with ValidPrime. Nucleic Acids Res. 2012; 40(7):e51. PMC: 3326333. DOI: 10.1093/nar/gkr1259. View

20.

Radi R . Oxygen radicals, nitric oxide, and peroxynitrite: Redox pathways in molecular medicine. Proc Natl Acad Sci U S A. 2018; 115(23):5839-5848. PMC: 6003358. DOI: 10.1073/pnas.1804932115. View