Epigenetic-structural Changes in X Chromosomes Promote Xic Pairing During Early Differentiation of Mouse Embryonic Stem Cells

Overview

Journal Biophys Physicobiol

Specialty Biophysics

Date 2022 Jul 7

PMID 35797402

Authors

Tetsushi Komoto

Masashi Fujii

Akinori Awazu

Affiliations

Soon will be listed here.

Abstract

X chromosome inactivation center (Xic) pairing occurs during the differentiation of embryonic stem (ES) cells from female mouse embryos, and is related to X chromosome inactivation, the circadian clock, intra-nucleus architecture, and metabolism. However, the mechanisms underlying the identification and approach of X chromosome pairs in the crowded nucleus are unclear. To elucidate the driving force of Xic pairing, we developed a coarse-grained molecular dynamics model of intranuclear chromosomes in ES cells and in cells 2 days after the onset of differentiation (2-day cells) by considering intrachromosomal epigenetic-structural feature-dependent mechanics. The analysis of the experimental data showed that X-chromosomes exhibit the rearrangement of their distributions of open/closed chromatin regions on their surfaces during cell differentiation. By simulating models where the excluded volume effects of closed chromatin regions are stronger than those of open chromatin regions, such rearrangement of open/closed chromatin regions on X-chromosome surfaces promoted the mutual approach of the Xic pair. These findings suggested that local intrachromosomal epigenetic features may contribute to the regulation of cell species-dependent differences in intranuclear architecture.

Citing Articles

Construction of Coarse-Grained Molecular Dynamics Model of Nuclear Global Chromosomes Dynamics in Mammalian Cells.

Awazu A, Komoto T Methods Mol Biol. 2024; 2856:281-292.

PMID: 39283459 DOI: 10.1007/978-1-0716-4136-1_17.

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