ST8SIA6-AS1 Promotes the Epithelial-to-Mesenchymal Transition and Angiogenesis of Pituitary Adenoma

Overview

Journal J Oncol

Specialty Oncology

Date 2022 Jul 5

PMID 35783150

Authors

Zuowei Li

Chengcheng Zhang

Xin Zong

Zhao Wang

Rong Ren

Lei Wang

Ping Sun

Chunmei Zhu

Mingxia Guo

Guizhen Guo

Guo Hu

Yanan Wu

Affiliations

Soon will be listed here.

Abstract

To investigate the effect of long noncoding RNA ST8SIA6-AS1 on the epithelial-to-mesenchymal transition (EMT) and angiogenesis of pituitary adenoma and its possible mechanism. The expression levels of ST8SIA6-AS1 and HOXA9 in noninvasive pituitary adenoma and invasive pituitary adenoma were detected using qRT-PCR. sh-ST8SIA6-AS1 transfection silenced the expression of ST8SIA6-AS1 in GH3 and GTI-1 cells. The effects of ST8SIA6-AS1 on the proliferation, invasion, angiogenesis, and EMT of GH3 and GTI-1 pituitary adenoma cells were detected. The migration ability of cells was detected through scratch assay. Dual luciferase analysis verified the targeting relationship between ST8SIA6-AS1 and miR-5195-3p. ST8SIA6-AS1 and HOXA9 were highly expressed in invasive pituitary adenoma. In pituitary adenomas, miR-5195-3p directly targeted HOXA9. miR-5195-3p is the target gene of ST8SIA6-AS1. ST8SIA6-AS1 knockdown inhibited the proliferation, invasion, angiogenesis, and EMT of pituitary adenoma. HOXA9 expression mediates the biological effect of ST8SIA6-AS1. ST8SIA6-AS1 targets miR-5195-3p to regulate the expression of HOXA9 and promote the EMT of pituitary adenomas.

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References

Fang Y, Pei Z, Chen H, Wang R, Feng M, Wei L . Diagnostic value of Knosp grade and modified Knosp grade for cavernous sinus invasion in pituitary adenomas: a systematic review and meta-analysis. Pituitary. 2021; 24(3):457-464. DOI: 10.1007/s11102-020-01122-3. View

Whelan J, Ludwig D, Bertrand F . HoxA9 induces insulin-like growth factor-1 receptor expression in B-lineage acute lymphoblastic leukemia. Leukemia. 2008; 22(6):1161-9. DOI: 10.1038/leu.2008.57. View

Guttman M, Rinn J . Modular regulatory principles of large non-coding RNAs. Nature. 2012; 482(7385):339-46. PMC: 4197003. DOI: 10.1038/nature10887. View

Lamouille S, Xu J, Derynck R . Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol. 2014; 15(3):178-96. PMC: 4240281. DOI: 10.1038/nrm3758. View

Micko A, Oberndorfer J, Weninger W, Vila G, Hoftberger R, Wolfsberger S . Challenging Knosp high-grade pituitary adenomas. J Neurosurg. 2019; 132(6):1739-1746. DOI: 10.3171/2019.3.JNS19367. View

Fu Z, Chen C, Zhou Q, Wang Y, Zhao Y, Zhao X . LncRNA HOTTIP modulates cancer stem cell properties in human pancreatic cancer by regulating HOXA9. Cancer Lett. 2017; 410:68-81. DOI: 10.1016/j.canlet.2017.09.019. View

Khorkova O, Hsiao J, Wahlestedt C . Basic biology and therapeutic implications of lncRNA. Adv Drug Deliv Rev. 2015; 87:15-24. PMC: 4544752. DOI: 10.1016/j.addr.2015.05.012. View

Johnson M, Woodburn C, Vance M . Quality of life in patients with a pituitary adenoma. Pituitary. 2004; 6(2):81-7. DOI: 10.1023/b:pitu.0000004798.27230.ed. View

Radisky D . Epithelial-mesenchymal transition. J Cell Sci. 2005; 118(Pt 19):4325-6. DOI: 10.1242/jcs.02552. View

10.

Shi X, Tao B, He H, Sun Q, Fan C, Bian L . MicroRNAs-based network: a novel therapeutic agent in pituitary adenoma. Med Hypotheses. 2012; 78(3):380-4. DOI: 10.1016/j.mehy.2011.12.001. View

11.

Xi X, Chu Y, Liu N, Wang Q, Yin Z, Lu Y . Joint bioinformatics analysis of underlying potential functions of hsa-let-7b-5p and core genes in human glioma. J Transl Med. 2019; 17(1):129. PMC: 6471881. DOI: 10.1186/s12967-019-1882-7. View

12.

McDowell B, Wallace R, Carnahan R, Chrischilles E, Lynch C, Schlechte J . Demographic differences in incidence for pituitary adenoma. Pituitary. 2010; 14(1):23-30. PMC: 3652258. DOI: 10.1007/s11102-010-0253-4. View

13.

Kalluri R, Weinberg R . The basics of epithelial-mesenchymal transition. J Clin Invest. 2009; 119(6):1420-8. PMC: 2689101. DOI: 10.1172/JCI39104. View

14.

Dave B, Mittal V, Tan N, Chang J . Epithelial-mesenchymal transition, cancer stem cells and treatment resistance. Breast Cancer Res. 2012; 14(1):202. PMC: 3496111. DOI: 10.1186/bcr2938. View

15.

Goncalves C, Xavier-Magalhaes A, Pojo M, Oliveira A, Correia S, Reis R . Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models. Genom Data. 2015; 5:54-8. PMC: 4583997. DOI: 10.1016/j.gdata.2015.05.010. View

16.

Xi X, Liu N, Wang Q, Chu Y, Yin Z, Ding Y . ACT001, a novel PAI-1 inhibitor, exerts synergistic effects in combination with cisplatin by inhibiting PI3K/AKT pathway in glioma. Cell Death Dis. 2019; 10(10):757. PMC: 6779874. DOI: 10.1038/s41419-019-1986-2. View

17.

Ezzat S, Asa S, Couldwell W, Barr C, Dodge W, Vance M . The prevalence of pituitary adenomas: a systematic review. Cancer. 2004; 101(3):613-9. DOI: 10.1002/cncr.20412. View

18.

Fang K, Hu C, Zhang X, Hou Y, Gao D, Guo Z . LncRNA ST8SIA6-AS1 promotes proliferation, migration and invasion in breast cancer through the p38 MAPK signalling pathway. Carcinogenesis. 2019; 41(9):1273-1281. DOI: 10.1093/carcin/bgz197. View

19.

Vierimaa O, Georgitsi M, Lehtonen R, Vahteristo P, Kokko A, Raitila A . Pituitary adenoma predisposition caused by germline mutations in the AIP gene. Science. 2006; 312(5777):1228-30. DOI: 10.1126/science.1126100. View

20.

Zhong W, Sun B, Gao W, Qin Y, Zhang H, Huai L . Salvianolic acid A targeting the transgelin-actin complex to enhance vasoconstriction. EBioMedicine. 2018; 37:246-258. PMC: 6286650. DOI: 10.1016/j.ebiom.2018.10.041. View