Unassigned Complex Unique Recombinant Forms Related to CRF36_cpx in Children Identified in an HIV-1 Outbreak in Pakistan

Overview

Journal AIDS Res Hum Retroviruses

Publisher Mary Ann Liebert

Specialty Infectious Diseases

Date 2022 Jul 2

PMID 35778855

Authors

Syed Hani Abidi

Dilsha Siddiqui

Syed Faisal Mahmood

Amna Rehana Siddiqui

Apsara Ali Nathwani

Aneeta Hotwani

Sharaf Ali Shah

Palwasha Khan

Rashida Abbas Ferrand

Fatima Mir

Affiliations

Soon will be listed here.

Abstract

In 2019, an outbreak of HIV infection predominantly affecting children occurred in Larkana district, Pakistan. This is the largest outbreak ever reported in this age group in Pakistan. In this study, we report two HIV-1 unique recombinant forms identified during the outbreak. Blood samples were collected from HIV-positive children as part of a case-control study to investigate the outbreak. The pol gene was sequenced and used to detect HIV subtype/recombinant forms using subtype, recombination, and phylogenetic analyses. Drug resistance mutation (DRM) analysis was performed to characterize the DRMs in each sequence. We observed the emergence of two unassigned unique recombinant forms related to CRF36_cpx in 15 individuals of the 344 samples collected. Genotype analysis revealed the presence of multiple DRMs associated with resistance to reverse transcriptase inhibitors. The discovery of these unassigned unique recombinant forms in our population highlights the need for comprehensive molecular epidemiological studies to fully understand the distribution and drug resistance patterns to aid control efforts.

Citing Articles

Characterization of HIV-1 CRF02_AG/A3/G unique recombinant forms identified among children in Larkana, Pakistan.

Rashid A, Kang L, Yi F, Mir F, Getaneh Y, Shao Y Front Cell Infect Microbiol. 2023; 13:1284815.

PMID: 37965253 PMC: 10642767. DOI: 10.3389/fcimb.2023.1284815.

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