Methylome-wide Analysis of IVF Neonates That Underwent Embryo Culture in Different Media Revealed No Significant Differences

Overview

Journal NPJ Genom Med

Specialty Genetics

Date 2022 Jun 29

PMID 35768464

Authors

Rebekka M Koeck

Florence Busato

Jorg Tost

Dimitri Consten

Jannie van Echten-Arends

Sebastiaan Mastenbroek

Yvonne Wurth

Sylvie Remy

Sabine Langie

Tim S Nawrot

Michelle Plusquin

Rossella Alfano

Esmee M Bijnens

Marij Gielen

Ron van Golde

John C M Dumoulin

Han Brunner

Aafke P A van Montfoort

Masoud Zamani Esteki

Affiliations

Soon will be listed here.

Abstract

A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration.

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At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale.

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