Using Genomic Deletion Mutants to Investigate Effector-Triggered Immunity During Legionella Pneumophila Infection

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 Jun 27

PMID 35759189

Authors

Rachelia R Wibawa

Pengfei Li

Kathleen McCaffrey

Elizabeth L Hartland

Affiliations

Soon will be listed here.

Abstract

Legionella pneumophila is an intracellular bacterial pathogen that uses a type IV secretion system (T4SS), termed Dot/Icm, to secrete more than 330 virulence effector proteins into the infected host cell. Many Dot/Icm effectors are involved in biogenesis of the Legionella-containing vacuole (LCV), which allows intracellular bacterial replication in environmental amoebae and alveolar macrophages. Through their activity, some effectors trigger the mammalian host immune response in a phenomenon termed effector-triggered immunity (ETI). Here, we describe a protocol to create and use L. pneumophila genome deletion mutants to identify effector(s) that alter pro-inflammatory cytokine production and bacterial clearance in the lungs of mice.

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