Endothelin B Receptor Immunodynamics in Pulmonary Arterial Hypertension

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2022 Jun 27

PMID 35757687

Authors

Christoph Tabeling

Carla R Gonzalez Calera

Jasmin Lienau

Jakob Hoppner

Thomas Tschernig

Olivia Kershaw

Birgitt Gutbier

Jan Naujoks

Julia Herbert

Bastian Opitz

Achim D Gruber

Berthold Hocher

Norbert Suttorp

Harald Heidecke

Gerd-R Burmester

Gabriela Riemekasten

Elise Siegert

Wolfgang M Kuebler

Martin Witzenrath

Affiliations

Soon will be listed here.

Abstract

Introduction: Inflammation is a major pathological feature of pulmonary arterial hypertension (PAH), particularly in the context of inflammatory conditions such as systemic sclerosis (SSc). The endothelin system and anti-endothelin A receptor (ET) autoantibodies have been implicated in the pathogenesis of PAH, and endothelin receptor antagonists are routinely used treatments for PAH. However, immunological functions of the endothelin B receptor (ET) remain obscure.

Methods: Serum levels of anti-ET receptor autoantibodies were quantified in healthy donors and SSc patients with or without PAH. Age-dependent effects of overexpression of prepro-endothelin-1 or ET deficiency on pulmonary inflammation and the cardiovascular system were studied in mice. Rescued ET-deficient mice (ET) were used to prevent congenital Hirschsprung disease. The effects of pulmonary T-helper type 2 (Th2) inflammation on PAH-associated pathologies were analyzed in ET mice. Pulmonary vascular hemodynamics were investigated in isolated perfused mouse lungs. Hearts were assessed for right ventricular hypertrophy. Pulmonary inflammation and collagen deposition were assessed lung microscopy and bronchoalveolar lavage fluid analyses.

Results: Anti-ET autoantibody levels were elevated in patients with PAH secondary to SSc. Both overexpression of prepro-endothelin-1 and rescued ET deficiency led to pulmonary hypertension, pulmonary vascular hyperresponsiveness, and right ventricular hypertrophy with accompanying lymphocytic alveolitis. Marked perivascular lymphocytic infiltrates were exclusively found in ET mice. Following induction of pulmonary Th2 inflammation, PAH-associated pathologies and perivascular collagen deposition were aggravated in ET mice.

Conclusion: This study provides evidence for an anti-inflammatory role of ET. ET seems to have protective effects on Th2-evoked pathologies of the cardiovascular system. Anti-ET autoantibodies may modulate ET-mediated immune homeostasis.

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